Figure 1 | Cell Death & Disease

Figure 1

From: Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Figure 1

LncRNA-POIR expression, which is significantly inhibited in inflammatory microenvironments, correlates with the osteogenic differentiation of pPDLSCs. (a) Heat map of differentially expressed lncRNAs (the top 10 in upregulated lncRNAs and the top 10 in downregulated lncRNAs) between hPDLSCs and pPDLSCs. (b) The results were confirmed using qPCR. (c) The expression levels of lncRNAs with high fold changes (fold change >5.0, P-value <0.05) were determined by qPCR at 7 days after osteogenic induction. (d) The expression levels of lncRNA-POIR were determined by qPCR at 0, 1, 7 and 14 days. (e) Correlation analysis between lncRNA-POIR levels and Runx2 and Col1 mRNA levels in pPDLSCs 0, 1, 7 and 14 days after osteogenic induction. All experiments were repeated three times. A total of six samples (three pPDLSCs and three hPDLSCs from six individuals) are tested in lncRNA profiling. Relative expressions of lncRNAs expression were normalized by β-actin in qPCR. All data are the mean±S.D. *P<0.05, **P<0.01 and NS, not significant. C, hPDLSCs; Con, control; lncRNA-POIR, pPDLSCs osteogenesis impaired-related lncRNA, ENST00000446358; Osteo, osteogenic induction; T, pPDLSCs; Undiff, without osteogenic induction

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