Figure 6

From: CD95 maintains stem cell-like and non-classical EMT programs in primary human glioblastoma cells

Figure 6

Tyrosine phosphorylation of CD95 recruits P85 and SFK and induces ncEMT. (a) PLA for tyrosine phosphorylation of the CD95 receptor in patient-derived CD95neg GBM cells transduced with a CD95 wild-type (CD95-WT) or CD95 mutant (tyrosine-to-alanine, CD95-mut) lentivirus stimulated with PBS (Co) or CD95L-T4. (b and c) Quantification of PLA results (t-test). (d and e) IP for CD95 and P-Tyrosine GSCs in naive, CD95-WT and CD95-mut GBM cells stimulated with CD95L-T4. Blots were probed with anti-P85 (regulatory PI3K subunit), anti-Sfk, anti-CD95 or anti-P-Tyrosine antibodies, respectively. (f) Expression of 62 genes expected to be upregulated during ncEMT measured by qPCR array in four FACS sorted as well as in a lentivirally transduced patient samples. Heat map displays log10 (CD95high or CD95wt)−log10 (CD95neg or CD95mut) values