c-Met and CREB1 are direct targets of miR-433. (a) qRT-PCR analysis. Decreased expression of c-Met, CREB1, GCLC, TGFßR1, and ARMC1 were observed in miR-433-transfected UM-UC-3 cells. (b) Dual-luciferase reporter assay. miR-433 significantly suppressed the luciferase activity of vectors that carried 3′-UTRs of c-Met and CREB1 but not GCLC, TGFβR1, and ARMC1. (c) Western blot analysis confirmed that miR-433 inhibited the endogenous expression of c-Met and CREB1. (d) The miR-433-targeting sites in 3′-UTRs of c-Met and CREB1 were mutated. (e) Dual-luciferase reporter assay. The luciferase activity of the mutated vectors were unaffected by the transfection of miR-433. Error bars represent the S.E. obtained from three independent experiments; *P<0.05.