Figure 3 | Cell Death & Disease

Figure 3

From: Notch signaling regulates myogenic regenerative capacity of murine and human mesoangioblasts

Figure 3

Dll1-triggered Notch signaling in MABs interplays with Maml1 and Mef2C and results in increased Mef2C activity. (a–d) Expression and protein levels of Mef2C/MEF2C and Maml1/MAML1 in murine and human MABs over time during spontaneous differentiation in vitro. Expression levels are reported in arbitrary units (AU) as fold change versus day 0. (e) Results of NICD-based immunoprecipitation (IP-NICD) at day 3 of differentiation of murine MABs in control conditions (ctrl), and in conditions of Dll1 knock-down (anti-Dll1), Dll1 overexpression (+Dll1), and combined overexpression of Dll1 and Mef2C (+Dll1/+Mef2C). Input, protein extract; IgG, mouse IgG-based IP, negative control; unbound, unbound fraction after IP. (f) Luciferase activity assayed at day 5 of differentiation after transfection with a plasmid carrying firefly luciferase expression under the control of a Mef2C-responsive element into murine MABs after Dll1 silencing (anti-Dll1), Dll1 overexpression (+Dll1), Mef2C overexpression (+Mef2C), and combined overexpression of Dll1/DLL1 and Mef2C/MEF2C (+Dll1/+Mef2C). *P<0.05 versus ctrl; §P<0.05 versus +Dll1/+DLL1 and +Mef2C/MEF2C (n=4). Depicted in (g, h) are the analogous results obtained with human MABs. Luciferase activity levels are reported in AU as fold change of renilla-normalized values versus ctrl conditions. Control cells were transduced with scramble and overexpression vectors and kept doxycycline-free. Data in charts are depicted as mean±standard deviation of ≥3 independent experiments. Scale bars indicate 100 μm

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