SMAR1 coordinates HDAC6-induced deacetylation of Ku70 and dictates cell fate upon irradiation

Acetylation status of DNA end joining protein Ku70 dictates its function in DNA repair and Bax-mediated apoptosis. Despite the knowledge of HDACs and HATs that are reported to modulate the acetylation dynamics of Ku70, very little is known about proteins that critically coordinate these key modifications. Here, we demonstrate that nuclear matrix-associated protein scaffold/matrix-associated region-binding protein 1 (SMAR1) is a novel interacting partner of Ku70 and coordinates with HDAC6 to maintain Ku70 in a deacetylated state. Our studies revealed that knockdown of SMAR1 results in enhanced acetylation of Ku70, which leads to impaired recruitment of Ku70 in the chromatin fractions. Interestingly, ionizing radiation (IR) induces the expression of SMAR1 and its redistribution as distinct nuclear foci upon ATM-mediated phosphorylation at serine 370. Furthermore, SMAR1 regulates IR-induced G2/M cell cycle arrest by facilitating Chk2 phosphorylation. Alternatively, SMAR1 provides radioresistance by modulating the association of deacetylated Ku70 with Bax, abrogating the mitochondrial translocation of Bax. Thus, we provide mechanistic insights of SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax.

Nuclear matrix (NM) is a fibrogranular network and an active site for various nuclear events, such as recombination, repair, splicing, transcription and so on. 1 NM functions as a scaffold for DNA double-strand break (DSB) repair as various repair factors are associated with its filamentous structure upon DNA damage. 2,3 Matrix attachment region-binding proteins (MARBPs) are unique class of proteins that bind to specific non-coding sequences in the genome termed as scaffold/ matrix attachment regions, and globally modify the topology of chromatin. 4 Scaffold/matrix-associated region-binding protein 1 (SMAR1) is one such MARBP, which was first identified in mouse double positive thymocytes. 5 SMAR1 exhibits transcriptional repression of multiple genes 6,7 and responds to various kinds of stress. 8,9 Ku70, a key player of non-homologous end joining (NHEJ) repair pathway, 10 associates with NM and acts as a docking factor to promote the tethering of free DSB ends to NM for repair. 3,[11][12][13] Posttranslational modification of many repair proteins has a prominent role in controlling the spatiotemporal dynamics of such factors at the site of damaged DNA. For example, modulation of Ku70 acetylation is a key switch between the two contrasting cellular fates upon stress: repair and death. [14][15][16] Ku70 acetylation inversely correlates with its DNA-binding property and repair efficiency. 17 Deacetylated Ku70 interacts and sequesters cytoplasmic pro-apoptotic protein Bax, 16,18 but the acetylation of Ku70 at its C-terminus leads to disruption of Ku70-Bax complex and mitochondrial translocation of Bax to induce apoptosis. 14,19 Positive regulation of cell survival upon stress is mediated through Ku70 deacetylation by various histone deacetylases, such as HDAC6, 17,18,20 SIRT1, 15 and SIRT3. 21 However, underlying mediator/regulatory proteins that modulate the deacetylation of Ku70 in response to stress remain enigmatic.
In the present study, we delineated a complex molecular mechanism of DNA damage repair and cell survival upon ionizing radiation (IR)-induced cellular stress. We found that SMAR1 is a novel interacting partner of Ku70 and mediates HDAC6-induced deacetylation of Ku70. Although it is established by various groups that HDAC6 deacetylates Ku70, we provide substantial evidence to prove the indispensability of SMAR1 for HDAC6-mediated Ku70 deacetylation. Multiple experiments establish that SMAR1, HDAC6 and Ku70 exist in the form of triple complex, with SMAR1 functioning as an intermediate bridge between HDAC6 and Ku70. We also show that upon IR, SMAR1 is phosphorylated at serine 370 by ATM and relocates to DSB sites. Furthermore, overexpression of SMAR1 favors IR-induced G2/M arrest, whereas its knockdown results in inefficient DNA repair and diminished cell survival. SMAR1 displays functional inhibition of Bax by regulating Ku70-Bax association. Together, our study demonstrates the novel role of SMAR1 in coordinating an intricate molecular mechanism upon DNA damage through modulation of Ku70 deacetylation. a stress-responsive protein, but least is known about its regulatory role during IR-induced DNA damage. Our initial observations in HCT116 cells revealed an induction in the expression of SMAR1 in a dose (Supplementary Figure S1a) and time-dependent manner upon IR (Figure 1a and Supplementary Figure S1b). Considering that the recruitment of certain factors to chromatin-associated DSB sites is a prerequisite for efficient repair, 22 we investigated the expression levels of SMAR1 in the chromatin and non-chromatin fractions upon irradiation. Results showed a considerable increase in the chromatin-associated SMAR1 upon IR (Figure 1b, lane 2 and Supplementary Figure S1c). Considering that Ku70, a key modulatory protein of NHEJ repair pathway, is recruited to chromatin upon IR, 23 we investigated its association with SMAR1. Immunoprecipitation (IP) assays in control and irradiated HCT116 cells (10 Gy,8 h) showed that SMAR1 interacts with Ku70 even in the absence of DNA damage (Figure 1c, lanes 5 and 6, respectively and Supplementary Figure S1d). Despite the discrepancies about Ku70 induction upon IR, some reports suggest increased expression of Ku70. 24 Similarly, we observed increased Ku70 in irradiated cells (Figure 1c, lane 2). The interaction of SMAR1 with Ku70 was further validated by reverse IP in HCT116 cells, in vivo interaction studies in different cell lines, such as HEK 293 and MCF-7 cells, and in vitro GST pulldown assays (Supplementary Figures S2a-d).
Further, we looked into the binding kinetics of SMAR1 with Ku70 upon DNA damage. Results showed that SMAR1 and Ku70 interaction gradually increases upon IR, reaching to a maximum extent by 12 h, and again restores to the basal level by 24 h (Figure 1d and Supplementary Figure S2e). Next, we explored the possibility of a DNA-mediated interaction between SMAR1 and Ku70 because both SMAR1 and Ku70 are enriched in chromatin-bound fractions upon IR. Surprisingly, interruption of DNA-protein interactions by using DNase I or ethidium bromide (EtBr) did not perturb the association of SMAR1 with Ku70 (Figure 1e), excluding the possibility of independent binding of SMAR1 and Ku70 to the damaged DNA and suggested the existence of SMAR1-Ku70 complex even in the absence of DNA damage. It is noted that interaction of Ku70 with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is DNA-dependent, 25 hence Figure 1 SMAR1 interacts with Ku70. (a) HCT116 cells were exposed to ionizing irradiation (10 Gy) followed by immunoblot analysis of SMAR1 at the indicated time points. Actin was used as a loading control. (b) Western blot analysis of SMAR1 and mentioned control proteins in chromatin and non-chromatin fractions of HCT116 cells that were either left untreated or irradiated (10 Gy, 2 h). (c) IP assay to check the interaction of SMAR1 with Ku70 upon IR (10 Gy, 8 h), wherein cell lysates were first immunoprecipitated with SMAR1 or control immunoglobulin G (IgG) followed by immunoblotting of the eluates with indicated antibodies, as shown. In all, 20% fraction of the whole-cell extract served as input control. (d) IP assay to study SMAR1-Ku70 association upon IR (10 Gy) in HCT116 cells at indicated time points. Graph depicts the densitometry quantification of timedependent interaction between SMAR1 and Ku70 using QuantityOne software (VersaDoc imaging system, BioRad). Control cell sample (as the value of 1) was used to normalize values of treated samples and data represents mean ± S.D. from three independent experiments. (e) Control and irradiated (10 Gy) HCT116 lysates were treated with either EtBr (50 μg/ml, 30 min) at 4°C or DNase I (100 U/ml, 20 min) at 37°C. Whole-cell lysates were immunoprecipitated with SMAR1 and then immunoblotted for Ku70 and SMAR1. (f) To study domain-specific interaction between SMAR1 and Ku70, HCT116 cells were transfected with Flag-tagged SMAR1 constructs (upper panel) and lysates were immunoprecipitated with anti-Flag antibody followed by immunoblotting with indicated antibodies (lower panel)  Figure S2f). We further mapped the domain of SMAR1 that associates with Ku70 by using a series of Flag-tagged full-length (F-SM) and deletion constructs of SMAR1. Results showed that SMAR1 associates with Ku70 through its protein-interacting domain (160-350 residues) (Figure 1f, lane 4). Neither the N-terminal region (1-160 residues) nor the DNA-binding domain (350-548 residues) of SMAR1 showed any interaction with Ku70 ( Figure 1f, lanes 3 and 5). Altogether, we conclude a specific crosstalk between SMAR1 and Ku70.
Acetylation of Ku70 has a modulatory role during DNA damage response. 17 Maintenance of p53 protein in a deacetylated state by SMAR1 through its interaction with HDAC1 7 intrigued us to analyze SMAR1-mediated modulation of Ku70 acetylation dynamics. Towards this, we performed acetylation-specific IP assays in HCT116 cells that were transduced with either SMAR1-adenovirus (Ad-SM) or SMAR1-ShRNA (sh3) lentivirus for the overexpression and knockdown of SMAR1, respectively. Results showed that SMAR1 maintains Ku70 in a deacetylated state, whereas knockdown of SMAR1-induced Ku70 acetylation by approximately fourfold (Figure 2a, right panel). Considering that SMAR1 does not harbor intrinsic deacetylase activity, we investigated the underlying mechanism of SMAR1-mediated Ku70 deacetylation. It is noted that HDAC6 deacetylates Ku70, 18 hence we explored for a possible co-existence of SMAR1, Ku70 and HDAC6 in one complex. Toward this, sequential IP assays in control HCT116 cells was performed, wherein whole-cell extracts were first immunoprecipitated with SMAR1 and the eluates were further pulled with HDAC6 and consequently probed for the presence of Ku70. Results showed an endogenous association of SMAR1, HDAC6 and Ku70 in a complex (Figure 2b). We further determined the association pattern of HDAC6-SMAR1-Ku70 complex at the molecular level by performing protein-protein docking analysis. The energy calculations of SMAR1, SMAR1-Ku70, SMAR1-HDAC6 and SMAR1-Ku70-HDAC6 complexes were carried out by using Macro-Model module of Maestro (Schrodinger, New York, NY, USA). From the energy content analysis, we observed that the electrostatic interactions due to formation of salt bridges between positively and negatively charged residues in the heterodimers and trimers predominates over all other van der Waals and solvent-solute interactions (Supplementary Table S1). The order of stability (highest to lowest) in different complexes have been found as (SMAR1+Ku70+HDAC6) Trimer 4 (SMAR1+Ku70) Dimer 4(SMAR1+ HDAC6) Dimer 4SMAR1 Monomer . The trimeric model of SMAR1 bound to HDAC6 and Ku70 revealed that 240-350 residues of SMAR1 interact with the N-terminal region of Ku70 through various inter residual salt In silico analysis of HDAC6-SMAR1-Ku70 docked model revealed that Ku70 is bound to SMAR1 adjacent to HDAC6-binding site (Figure 2c). It was observed that C-terminal domain (248-371 residues) of SMAR1 is sandwiched between Ku70 and HDAC6. To further substantiate SMAR1-mediated interaction of Ku70 with HDAC6, we checked the Ku70-HDAC6 association upon SMAR1 knockdown. Results showed that the specific interaction of HDAC6 with Ku70 was retained in cells transduced with non-silencing (NS) lentivirus; however, the knockdown of SMAR1 with sh3 lentivirus perturbed the interaction (Figure 2d).
Altogether, these data suggest that SMAR1 has a critical role in HDAC6-mediated deacetylation of Ku70. In order to further validate SMAR1-mediated Ku70 deacetylation via HDAC6, we investigated the effect of HDAC6-specific inhibitor tubacin on the Ku70 deacetylation. IP assays revealed that tubacin partially hampered SMAR1-mediated Ku70 deacetylation, suggesting that SMAR1 coordinates Ku70 deacetylation via HDAC6 (Figure 2e, lane 3). As deacetylated form of Ku70 is reported to be efficiently recruited to the sites of damaged DNA, we then checked Ku70 enrichment in the chromatin fractions of NS and sh3 lentivirus-transduced HCT116 cells upon IR. The recruitment of Ku70 to chromatin was convincingly impeded in the absence of SMAR1 (Figure 2f, right panel). Furthermore, recruitment of Ku70 to laser microirradiation-induced DNA damage sites was severely impaired upon siRNA-mediated ablation of SMAR1 (Supplementary Figures S3a and b), which further affirms that SMAR1 affects the ability of Ku70 to bind DSB sites. Together, these results suggest that SMAR1 functions as a mediator for HDAC6-induced deacetylation of Ku70 and thus facilitates its chromatin recruitment.
ATM is indispensable for SMAR1 function upon DNA damage. The proteins such as 53BP1, Mre11, BRCA1 and so on are recruited to the sites of DNA damage and form IRinduced foci. [26][27][28] Considering the enrichment of SMAR1 in the chromatin fraction upon IR, we checked for IR-induced relocalization of SMAR1 in the nucleus. Immunofluorescence analysis of SMAR1 in HCT116 cells revealed that it forms distinct nuclear foci as early as 5 min post IR ( Figure 3a). Interestingly, these foci were found to colocalize with IRinduced γH2AX foci. To further corroborate the recruitment of SMAR1 to DSB sites, we employed laser microirradiation approach to introduce DNA damage in a distinct tract (laser line) inside the cell nucleus. Understandably, we observed enrichment of SMAR1 as well as its colocalization with γH2AX along the laser-induced damaged DNA tracts (Supplementary Figure S4a). Furthermore, IP assays in irradiated HCT116 cells indicated the association of SMAR1 with γH2AX ( Figure 3b and Supplementary Figure S4b), suggesting that SMAR1 might function as an early-response protein upon DNA damage. Furthermore, we investigated whether ATM kinase affects the redistribution of SMAR1 to such foci. To test this, HCT116 cells were treated with ATM kinase inhibitor KU-55933 (10 μM, 2 h) before irradiation. Results showed abrogation of IR-induced SMAR1 foci formation upon ATM inhibition (Figure 3c). Taken together, these data indicate that SMAR1 relocalizes inside the nucleus to form foci in response to DNA damage in an ATM kinase-dependent manner.
Next, we examined whether SMAR1 is a substrate of ATM kinase. Initially, scanning of SMAR1 amino-acid sequence revealed the presence of a consensus ATM phosphorylation site SQ (a serine followed by glutamine; Figure 3d). 29 To establish ATM-mediated phosphorylation of SMAR1, in vitro kinase assays were performed with recombinant fulllength GST-SMAR1 and various truncations, such as GST-SMAR1 (160-350 residues), GST-SMAR1 (350-548 residues) and GST-SMAR1 (400-548 residues). Results showed the phosphorylation of full-length SMAR1 as well as GST-SMAR1 (350-548 residues), but not GST-SMAR1 (160-350 residues) and GST-SMAR1 (400-548 residues) (Figure 3e, lanes 1 and 3), which can further be explained owing to the presence of a single SQ motif in the 350-400 region of SMAR1 (see also Figure 3d). To further confirm IR-induced ATM-mediated phosphorylation of SMAR1, we generated antiphosho-SMAR1 antibody that specifically recognizes serine 370 phosphorylated form of SMAR1 (p-SMAR1). Time-course study for SMAR1 phosphorylation in irradiated HCT116 cells using p-SMAR1 antibody indicated that phosphorylation of SMAR1 begins as early as 5 min upon IR (Figure 3f, lane 2). IR dose, as low as 0.5 Gy, induced significant phosphorylation of SMAR1, although we observed a proportionate increase in p-SMAR1 with the higher doses of IR (Supplementary Figure  S4c, lanes 2 and 7). The specificity of phospho-antibody was further illustrated by probing the Flag-immunoprecipitated eluates from control and irradiated HCT116 cells that were exogenously expressed with either F-SM or phosphorylationdeficient S370A SMAR1 mutant (F-Mut, substituting serine with alanine). The presence of a specific band in irradiated F-SM-transfected cells, but failure of phospho-SMAR1 antibody to recognize mutant SMAR1 (Supplementary Figure  S4d, lanes 2 and 4), highlighted the specificity of the antibody and established that serine 370 is a potential site for IR-induced phosphorylation of SMAR1 by ATM kinase. The association of ATM with wild-type SMAR1, but not with phosphorylation-deficient (S370A) SMAR1 in irradiated HCT116 cells (Figure 3g), suggested that ATM directly associates with SMAR1 by recognizing serine 370 as its consensus phosphorylation site and mutation of this site abrogates the ATM association with SMAR1, hence mutant SMAR1 remains unphosphorylated.
The irradiated ATM +/+ mouse embryonic fibroblasts (MEFs) and human A-T lymphoblastoid control C3ABR cells showed phosphorylation of SMAR1, whereas ATM − / − MEFs and ATM-deficient lymphoblastoid L3 cells exhibited complete abrogation of SMAR1 phosphorylation (Figures 3h and i). As ATM-mediated DNA damage signaling is considered to be indispensable for efficient recruitment of repair proteins to DSB sites, 30 we wondered whether the redistribution of SMAR1 to damage-associated chromatin fraction is ATMdependent. Results showed that treatment with ATM inhibitor KU-55933 ( Figure 3j) and PI3 kinase inhibitor caffeine (Supplementary Figure S4e), before irradiation, substantially reduced the chromatin enrichment of SMAR1. Altogether, results from these experiments strongly indicated that ATMmediated DNA damage signaling is essential for the recruitment of SMAR1 to the site of damaged DNA. and Supplementary Figure S5). On the contrary, knockdown of SMAR1, in combination with IR, resulted in increased chromosomal aberrations, highlighting the indispensability of SMAR1 for repair upon IR-induced DNA damage. To comprehensively elucidate the role of SMAR1 in DNA damage repair, we performed an in vivo end joining assay using pGL2-Luc vector, a microhomologous DNA damage repair reporter system. In this assay, HindIII-or EcoRIlinearized pGL2-Luc vector was transfected in HCT116 cells that were previously transduced with either Ad-SM or sh3 lentivirus. After 48 h of transfection, end joining efficiency was estimated by measuring luciferase expression that will only be achieved once plasmid is precisely rejoined in its circular form. Of note, EcoRI cleaves within the coding region of luciferase gene, therefore precise end joining of the short overhangs involves microhomology-directed DNA repair to restore Luc activity. On the contrary, HindIII cuts inside the linker region located between the SV40 promoter and Luc coding sequence, hence precise end joining is dispensable for restoration of Luc activity. Quantification of Luc activity in SMAR1 overexpressed cells that were transfected with EcoRI-linearized pGL2 plasmid DNA showed significant recovery (74%), whereas SMAR1 knockdown counterpart showed only 38% recovery in Luc activity ( Figure 4b).
However, simple end ligation, as occurs in the case of HindIII-linearized plasmids, is not affected by knockdown of SMAR1. In summary, these results strongly emphasized the crucial role of SMAR1 in the repair of damaged DNA.
Considering that accurate repair of damaged DNA requires the arrest of cell cycle progression, 31 we investigated the role of SMAR1 in altering cell cycle checkpoints in response to IRinduced DNA damage. Towards this, HCT116 cells that were previously transduced with Ad-SM or sh3 lentivirus were either left untreated or irradiated (10 Gy) and subsequently stained with propidium iodide (PI). Analysis of different phases of cell cycle indicated that SMAR1 positively regulates the onset of IR-induced G2/M checkpoint as evidenced by increased 4N-DNA content in cell population (Figure 4c and Supplementary Figure S6). Strikingly, SMAR1-deficient cells failed to exhibit G2/M checkpoint, and instead showed elevated apoptotic population. We further set out to decipher the molecular mechanism underlying SMAR1-mediated G2/M arrest. Earlier reports attributed Chk2 as a key regulatory checkpoint kinase that is phosphorylated at threonine 68 by ATM in response to IR and regulates G2/M transition. 32 To check whether SMAR1 regulates IR-induced G2/M arrest by modulating Chk2 phosphorylation, we performed immunoblot analysis in control and SMAR1 overexpressed HCT116 cells. Our results demonstrated that SMAR1 overexpression is associated with enhanced Chk2 phosphorylation upon IR (Figure 4d). Moreover, we observed a concomitant increase in the phosphorylation of downstream G2/M checkpoint regulators, such as Cdc25C and Cdc2. However, we did not observe any change in the phosphorylation of Chk2 upon SMAR1 overexpression in control cells (Figure 4d, lane 3), strongly suggesting that SMAR1 induces G2/M arrest only upon IR-induced stress. Decreased phosphorylation of histone H3 at serine 10, further supported that indeed SMAR1 delays progression of cells from G2 to M phase upon IR. To further ascribe ATMdependent functionality of SMAR1-induced cell cycle arrest, SMAR1 overexpressed cells were incubated with KU-55933 before IR. Results from these experiments indicated that SMAR1-mediated IR-induced G2/M arrest was reversed upon ATM inhibition (Figure 4d, lane 5), affirming the indispensability of ATM for the efficient functioning of SMAR1 in To further correlate the levels of Chk2 phosphorylation with its kinase activity, we performed Chk2 kinase activity assays. Results showed significantly enhanced kinase activity of immunoprecipitated phospho-Chk2 in irradiated HCT116 cells that were overexpressed for SMAR1 (Figure 4e). Together, these data strongly implicate SMAR1 as a key cell cycle regulator, especially during G2/M checkpoint upon DNA damage to ensure efficient repair.

SMAR1 regulates IR-induced apoptosis and orchestrates
Ku70-Bax interaction. Critical analysis of our cell cycle data indicated enhanced apoptosis in irradiated cells that were knockdown for SMAR1. To further substantiate IRinduced apoptosis upon SMAR1 knockdown, we performed colony formation assays in control or irradiated SMAR1 overexpressed and knockdown HCT116 cells. Examination of colony formation by crystal violet staining revealed that SMAR1 overexpressed cells exhibited increased radioresistance, whereas SMAR1 knockdown cells showed enhanced apoptosis ( Figure 5a). Next, we assayed cellular apoptosis using MitoCapture apoptotic detection kit (Calbiochem, San Diego, CA, USA) and quantification of apoptotic populations revealed that SMAR1-deficient cells are highly sensitive to IR, as evidenced by 46.05% apoptotic population (Figure 5b and Supplementary Figure S7). On the other hand, SMAR1 overexpressed cells showed only 10.04% apoptotic population upon IR. Altogether, these results conclusively underscore the significance of SMAR1 in imparting resistance toward IR-induced stress. In accordance with these observations, we performed whole-body irradiation (7 Gy) experiments in SMAR1-overexpressing transgenic, SMAR1 heterozygous knockout and wild-type littermate mice to highlight the crucial role of SMAR1 in survival upon DNA damage. Interestingly, 40% of SMAR1 transgenic mice survived even after 20 days of IR, whereas SMAR1 heterozygous knockout mice died within 9 days of IR ( Figure 5c). We further evaluated the expression pattern of apoptotic markers upon SMAR1 knockdown. Results indicated an increase in the expression of cytochrome c, active caspase-3 and cleaved PARP upon SMAR1 knockdown and this increase was further manifested upon IR (Figure 5d). Altogether, above results indicated that SMAR1 generates a strong anti-apoptotic response upon genotoxic stress.
Earlier studies have reported that acetylation of Ku70 alters its association with Bax, which translocates to mitochondria and induces cellular apoptosis. 14 As SMAR1 modulates the acetylation status of Ku70, we investigated for any possible correlation between SMAR1 expression and Ku70-Bax association. IP assays in the HCT116 cells that were either  (Figure 5e, lane 3). This perturbation in Ku70-Bax association can be plausibly explained by the enhanced acetylation of Ku70 upon SMAR1 knockdown. It is noted, once Bax is free from its association with Ku70, it translocates to mitochondria and initiates apoptosis. 14 Considering that SMAR1 positively regulates the association of Ku70 with Bax, we further reasoned that SMAR1 modulates the cellular localization of Bax. Towards this, western blot analysis of subcellular fractions revealed impaired mitochondrial translocation and cytoplasmic retention of active Bax (6A7) in SMAR1 overexpressed HCT116 cells (Figure 5f, lanes 5 and 11). In contrast, we observed enrichment of Bax in the mitochondrial fractions of SMAR1 knockdown cells, which further increased upon IR (Figure 5f, lanes 9 and 12). It is worth mentioning here that reduced expression of Bax in the cytoplasmic fraction upon SMAR1 overexpression is attributed to SMAR1mediated transcriptional repression of Bax. 7 Overall, our results convincingly demonstrate that SMAR1 regulates cell survival upon DNA damage by modulating the association of Ku70 and Bax.

Discussion
Our findings highlight the significance of Ku70-SMAR1-HDAC6 association, which underlines an intricate crosstalk between the various regulators and eventually determines the cell fate. Till date, many proteins have been reported to interact with Ku70 and thereby regulate either DNA damage repair or apoptosis. 20,33,34 Although various HATs and HDACs have been proposed to have a significant role in the modulation of Ku70 acetylation or deacetylation, 14,18,19 the involvement of mediator proteins in Ku70 acetylation still remain obscure. Notably, our study for the first time provides evidences of an unidentified mechanism for cell survival that involves SMAR1mediated fine tune regulation of Ku70 deacetylation through HDAC6, which subsequently results in efficient DNA repair and reduced apoptosis (Figure 6a). A step ahead, SMAR1 was found to be essential for efficient recruitment of Ku70 to damage-associated chromatin fraction to carry out repair, as evidenced by increased chromosomal aberrations and defective DNA repair upon SMAR1 knockdown (Figure 6b). Here, marked reduction in the repair efficiency upon SMAR1 knockdown could be attributed to perturbation of SMAR1-Ku70-HDAC6 triple complex and subsequent increase in Ku70 acetylation, leading to defective binding of acetylated Ku70 to damaged DNA.
One of the earliest events in the aftermath of DNA damage is the ATM-mediated phosphorylation of various factors, which in most cases is followed by distinct foci formation of such phosphorylated proteins. 28 These foci contain numerous molecules of repair proteins and close proximity of all key players ensures efficient repair. IR-induced activation, ATMmediated phosphorylation of SMAR1 followed by its relocalization to DSB sites, and colocalization with γH2AX strongly affirms the crucial role of SMAR1 in early response upon DNA damage.
Activation of checkpoint regulators induces arrest in ongoing cell cycle that provides necessary time for repair of damaged DNA before the transmission of wrong genetic information to following generations. In this study, we observed that upon IR-induced DSB generation, SMAR1 favors G2/M arrest via increased phosphorylation of Chk2 at threonine 68. We hypothesize that, being a MARBP, recruitment of SMAR1 near DSB sites might serve as a scaffold for interaction between the components of DDR signaling that favors the induction of checkpoints. Notably, DNA-PK augments the phosphorylation of Chk2 by ATM, 35 hence increased phosphorylation of Chk2 upon SMAR1 overexpression can be reasoned to enhanced recruitment of Ku70 to DSB sites, which results in the formation of holoenzyme DNA-PK. Furthermore, recruitment of SMAR1, Ku70 and other repair factors near damaged DNA might induce crosstalk between the closely juxtaposed multi-subunit signal transduction complexes, resulting in the amplification of DNA damage signal, and activation of checkpoint regulators and downstream effector proteins such as Cdc25c and Cdc12, which allow sufficient time for a cell to accurately repair damaged DNA.
This study further highlighted that SMAR1 is indispensable for survival upon DNA damage, whereas its deficiency leads to increased apoptosis. SMAR1 has already been shown to repress various pro-apoptotic genes such as Bax and Puma. 7 However, the alternate mechanism by which SMAR1 manifests its anti-apoptotic function is entirely novel in this study. On one hand, SMAR1 regulates the expression of Bax at transcriptional level by simultaneously inducting p53 deacetylation through HDAC1 to generate anti-apoptotic response. Whereas, SMAR1 also displays posttranslational regulation and functional inhibition of Bax by deacetylation of Ku70 via HDAC6 that subsequently strengthens the Ku70-Bax association, preventing apoptotic translocation of Bax to mitochondria. Here, an interesting question arises that why SMAR1 exhibits an alternate mode of regulation on the function of Bax. One potential answer is that cell needs additional players, which salvage the emergency situation during genotoxic stress by providing efficient cell recovery alternatives. Therefore, it is highly possible that retention factors, such as SMAR1, function in synergistic manner to prevent the relocation of Bax upon DNA damage, even before it is transcriptionally shut down.
In summary, we propose that SMAR1 is a master regulator of cell fate in response to IR-induced DNA damage and our findings highlight the significance of Ku70-SMAR1-HDAC6 association that underlies a complex crosstalk between the various regulators that eventually determine the cell fate. Understanding this rheostat system, which critically balances the cell fate, will be helpful in rationalizing the modes of cell survival, attempting to develop novel and advanced therapeutic tools.

Materials and Methods
Cell culture, plasmids and mice. HCT116, HEK 293T, MCF-7 and ATM-MEFs were cultured in DMEM medium (Invitrogen, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 U/ml of antibiotic (penicillin-streptomycin; Invitrogen). A-T lymphoblastoid cell lines L3 (ATM deficient) and C3ABR (ATM proficient) were cultured in RPMI medium (Invitrogen). SMAR1 transgenic and wild-type mice were maintained as previously described 36 and all experiments were performed as per guidelines of animal ethical committee, NCCS. SMAR1 heterozygous knockout mice were generated at Ozgene (Perth, WA, Australia). Exon 2 of SMAR1 was flanked by loxP sites and a phosphoglycerate kinase-neo cassette flanked by FLP recombinase target sites was used for selection. Following homologous recombination of the vector in C57BL/6 embryonic stem cells and establishment of germline transmission, the PGK-neo cassette was excised using the FLP recombinase, leaving exon 2 flanked by loxP sites. As homozygous SMAR1 knockout mice were embryonic lethal, heterozygous knockout mice were used for experimental purposes. Transfection of full-length p3X-Flag-SMAR1, truncations of SMAR1 with p3X-Flag back bone (1-160, 160-350 and 350-548 residues) and serine 370 alanine mutant of SMAR1 (generated by using site-directed mutagenesis kit, Stratagene, La Jolla, CA, USA) were carried out in Opti-MEM medium (Invitrogen) supplemented with 0.5% FBS using Lipofectamine 2000 (Invitrogen). Control and custom synthesized siRNA for SMAR1 (Ambion, San Diego, CA, USA) were used at 300 nM concentration for 24 h. To induce DNA damage, cells were exposed to γ-irradiation using gamma chamber with 137 Cs source as per indicated doses. Whenever needed, ATM inhibitor KU-55933 (10 μM; Tocris Bioscience, Bristol, UK) was added to the media 2 h before IR. HDAC6specific inhibitor tubacin (5 μM; Sigma, St. Louis, MO, USA) was added to media and incubated for 1 h.
Immunofluorescence analysis and laser microirradiation. Cells were processed for immunofluorescence analysis as previously described. 7 Slides were observed under the fluorescence microscope (Carl Zeiss Axio Imager.Z2, Jena, Germany). For microirradiation studies, cells were subjected to localized damage in the form of specific laser tracts through a UV-A laser beam. Cells were seeded in multi-chamber glass slides (Nunc, Rochester, NY, USA) and presensitized with 10 μM 5-bromo-2′-deoxyuridine (Sigma) in phenol red-free medium (Invitrogen) for 24 h. Laser microirradiation was performed by Axio observer D1 microscope (Carl Zeiss), which is supplemented with PALM microbeam. Cells were exposed to 405 nm laser diode (6 mW) using PALM robo software 4.3 SP1 (Jena, Germany), focused through a × 40 objective to yield a spot size of 0.5-1 mm. The time of exposure was set in fast scanning mode with laser settings in such a range to generate a detectable laser path without causing physical damage to the cells. After microirradiation, imaging of cells was done as described above. Immunostaining for Ku70 was done as described previously. 37 In vitro kinase assay. In vitro kinase assay was performed as described earlier. 38 Briefly, endogenous ATM was immunoprecipitated for an hour at 4°C with protein A/G beads from the cleared supernatants of the irradiated (10 Gy) cells that were lysed in modified TGN buffer (50 mM Tris pH 7.5, 50 mM β-glycerophosphate, 150 mM NaCl, 1mM dithiothreitol (DTT), 1% Tween-20, 0.3% Nonidet P-40, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1mM sodium fluoride) supplemented with 1 × protease inhibitor cocktail (Roche). After one wash with TGN buffer, the beads were subsequently washed twice in kinase buffer (10 mM HEPES pH 7.5, 50 mM β-glycerophosphate, 50 mM NaCl, 10 mM MgCl 2 , 1 mM DTT and 10 mM MnCl 2 ). Finally the immunoprecipitates were resuspended in kinase buffer containing 5 μM ATP, 10 μCi of (γ-32 P) ATP and 1 μg purified GST-fusion substrates, and incubated for 30 min at 30 ºC. The kinase reaction was stopped by adding 2 × SDS loading buffer followed by 12% SDS-PAGE and autoradiography.
Cell cycle analysis and apoptosis assays. Cells were trypsinized and fixed with 80% chilled ethanol for 2 h at 4 ºC. After treatment with RNase A (10 μg/ml; Sigma), cells were stained with PI (Sigma) followed by acquisition and analysis by FACS calibur cytometer (BD Biosciences, San Jose, CA, USA) using Cell Quest software (BD Biosciences). Apoptosis assays were performed using MitoCapture apoptosis detection kit (Calbiochem) as per the manufacturer's instructions. Briefly, cells were harvested and stained with MitoCapture dye followed by flow cytometry. Healthy cells, wherein the aggregates of dye in the mitochondria emit red fluorescence, are detected in the PI (FL2) channel, whereas apoptotic cells are detected in the FITC (FL1) channel due to green fluorescence of dye monomers that are dispersed throughout the cell. All assays were carried out in triplicates. Chk2 kinase activity assay. Analysis of checkpoint kinase activity was performed using K-LISA checkpoint activity kit (Calbiochem) according to the manufacturer's protocol. Briefly, Chk2 was immunoprecipitated from the lysates of HCT116 cells and immunoprecipitates were incubated with biotinylated peptide substrate in the presence of ATP. Phosphorylated substrate is detected with phosphoserine-specific antibody and kinase activity is determined using colorimetric detection.
In vivo NHEJ assay. In vivo NHEJ assay using plasmid substrates was done as described. 41 Briefly, pGL2 vector was linearized with either HindIII or EcoRI, and the linearized fragment was purified by gel extraction method, estimated and transfected. After 48 h of transfection, cells were harvested and assayed for luciferase activity.
Metaphase spread analysis. Metaphase spreads were prepared as previously described. 42 Briefly, cells were arrested at metaphase by adding 100 ng/ml of colcemid (Sigma) to the culture media, followed by incubation with prewarmed hypotonic solution and fixation (methanol:acetic acid in 3 : 1). Finally, 20-50 μl of cell suspension was dropped on a humidified glass slide. Slides were subsequently stained with Giemsa stain (Sigma) and metaphase spreads were observed under bright field microscope (Carl Zeiss Axiovert 200 M) using × 100 objective.
Protein-protein docking analysis. To perform protein-protein docking of HDAC6, SMAR1 and Ku70, the structural coordinates of HDAC6 (3C5K) 43 and Ku70 (1JEQ) 10 were obtained from PDB database. Crystal structure of free domain of Ku70, which is not bound to Ku80, was extracted from the Ku70:Ku80 complex. Next, the structure of SMAR1 was built with I-TASSER online server (Michigan, MI, USA). 44 The structural coordinates of all the three systems were optimized in the protein preparation wizard of Maestro. 45 All the missing hydrogen atoms were added and further subjected to energy minimization with OPLS-2005. The interaction models for SMAR1, HDAC6 and Ku70 were obtained with ZDOCK (v.3.0.2; University of Massachusetts, Worchester, MA, USA), which predicts the interacting models by the fast Fourier transform and allows 3D searches of spatial degrees of freedom between the macromolecules. 46 The predicted docked models of HDAC6-SMAR1-Ku70 were further analyzed with the PyMol (Schrodinger). 47