Figure 1 | Cell Death & Disease

Figure 1

From: Fluvoxamine alleviates ER stress via induction of Sigma-1 receptor

Figure 1

Fluvoxamine (Flv) induces the expression of sigma-1 receptor (Sig-1R). (a) Flv (10 μg/ml) was added to Neuro2a cells, and Sig-1R protein and GAPDH protein levels were monitored over the time course (h) shown in the figure. (b) The amount of Sig-1R and GAPDH protein in (a) was quantified with densitometry. Relative intensity of Sig-1R/GAPDH increased significantly in response to Flv treatment. The 0 h time point was normalized to 1. Values shown are the mean±S.D. (*P<0.05, Student’s t-test; n=3). (c) Sig-1R and β-actin mRNA expression in Neuro2a cells treated with 10 μg/ml Flv was measured by semiquantitative RT-PCR over the time course shown in the figure. The Sig-1R mRNA level increased by 3.8-fold after Flv treatment for 24 h. (d) Sig-1R and β-actin mRNA expression in Neuro2a cells treated with 10 μg/ml Flv was determined by quantitative RT-PCR over the time course shown in the figure. The Sig-1R mRNA level increased by 32% and 28% after Flv treatment for 12 h and 24 h, respectively. The 0 h time point was normalized to 1. Values are the mean±S.D. (*P<0.05, Student’s t-test; n=3). (e) The reporter assay system shown in the figure was constructed. The Sig-1R promoter region (-582 to -156) was fused to a firefly luciferase plasmid (pGL4.12[luc2CP]) and relative luciferase activities were measured. Luciferase activity increased significantly over the time course shown in response to treatment with 10 μg/ml Flv. Values are the mean±S.D. (*P<0.05, Student’s t-test; n=3)

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