Figure 4 | Cell Death & Disease

Figure 4

From: Cancer-derived immunoglobulin G promotes tumor cell growth and proliferation through inducing production of reactive oxygen species

Figure 4

Downregulation of cancer-derived IgG enhanced cellular total antioxidant capacity. (a) HeLa cells were treated with IGHG1 siRNA or scrambled siRNA for 72 h. T-AOC was measured in 593 nm with FRAP method with enzyme-labeling analyzer. Similar experiments were performed for HEp-2 and PC3 cells (b). After knockdown of IGHG1 in HeLa cells, the expression of PRDX1 at both protein levels (c) and mRNA levels (d) were measured. In addition, the activities of total SOD (e), Mn-SOD (f) and CuZn-SOD (g) were analysed in 450 nm with WST-1 method by enzyme-labeling analyzer. (h) CAT activity was measured in 520 nm by enzyme-labeling analyzer. GR activity (i) and GPx activity (j) were measured in 340 nm by enzyme-labeling analyzer. (k) GSH content was detected in 412 nm. Similar experiments were performed using HeLa cells stably expressing IGHG1 shRNA or control shRNA (a, ck). The data of T-AOC, enzyme activities and RT-qPCR are presented as the mean±S.D. of three independent experiments (*P<0.05; **P<0.01)

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