Figure 3 | Cell Death & Disease

Figure 3

From: Cancer-derived immunoglobulin G promotes tumor cell growth and proliferation through inducing production of reactive oxygen species

Figure 3

Cancer-derived IgG interacted with RACK1, RAN and PRDX1 in the cytoplasm of HeLa cells. (a) Cell lysates were immunoprecipitated with mouse anti-human IgGγ antibody or normal mouse IgG. The immunoprecipitates and whole-cell lysate were fractionated by SDS-PAGE and blotted with anti-RACK1, anti-RAN, anti-PRDX1 and anti-IgGγ antibodies. (b) HeLa cell lysates were immunoprecipitated with mouse anti-RACK1 antibody and normal mouse IgG, the immunoprecipitates and whole-cell lysate were fractionated by SDS-PAGE and blotted with anti-RACK1, anti-RAN or anti-PRDX1 antibody. (c) HeLa cell lysates were immunoprecipitated with goat anti-PRDX1 antibody and normal goat IgG, the immunoprecipitates and whole-cell lysate were fractionated by SDS-PAGE and blotted with anti-RAN. (d and e) HeLa cells were fixed and double-stained for IgGγ, RACK1 or PRDX1 (green) and RACK1, RAN or PRDX1 (red). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). The merged images (overlay, yellow) were viewed with a confocal microscope and shown in the right panel (Scale bar, 20 μm)

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