Figure 6 | Cell Death & Disease

Figure 6

From: TGF-β1 exposure induces epithelial to mesenchymal transition both in CSCs and non-CSCs of the A549 cell line, leading to an increase of migration ability in the CD133+ A549 cell fraction

Figure 6

Migration potential analyses. (a) Migration assay showed that TGF-β1 significantly enhanced the migration of all samples compared with their controls except for CD133 fraction. (b) RT-PCR and densitometry analyses showed that MMP-9 gene expression levels were increased in all sorted fractions except for the CD133 fraction. The CD133+ fraction showed higher levels of MMP-9 gene expression than other fractions. (c) Western blot analyses for MMP-9 confirmed RT-PCR results. β-Actin is used as loading control. **P<0.0005, ***P<0.0001 compared to the parental cell line (day 0 of treatment)

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