Figure 2 | Cell Death & Disease

Figure 2

From: Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis

Figure 2

Preferential role of the Bim/Bcl-2 axis in controlling iTreg survival. (a and b) Tconv were isolated from WT or Bim−/− Foxp3GFP mice and activated with anti-CD3/CD28-coated beads in the presence of TGF-β (5 ng/ml) and IL-2 (100 U/ml) for 3 days to induce iTreg differentiation. The iTregs (GFP+) and activated Tconv (GFP) were then sorted by FACS and cultured for additional 3 days in the absence of IL-2. Percentage of live iTregs and Tconv was determined by flow cytometry with propidium iodide (PI) and anti-Annexin V. Data are the mean±S.E. of four independent experiments with statistical analysis performed using standard paired t-test (*P<0.05). (c) iTregs (GFP+) and activated Tconv (GFP) were cultured in the presence of various IL-2 concentrations for 3 days and percentage of live cells determined by PI and anti-Annexin V. Data is representative of two independent experiments

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