Figure 2 | Cell Death & Disease

Figure 2

From: TIAF1 self-aggregation in peritumor capsule formation, spontaneous activation of SMAD-responsive promoter in p53-deficient environment, and cell death

Figure 2

TIAF1 aggregation in the interface between metastatic cancer cells and the brain tissues. (a) Presence of aggregated TIAF1 in the dead neurons located in the interface between the brain tissue and the metastatic small-cell lung cancer. Degenerating neurons were stained with Fluoro-Jade C (red). Nuclei were stained with DAPI. Scale bar, 20 μm; × 400 magnification. A merged image is of TIAF1 (green), Fluoro-Jade C (red) and DAPI (blue). The blocking R48-2 peptide was used in negative controls. Also, see Supplementary Figures S7 and S8. (b) TIAF1/Aβ aggregates are shown in a metastatic lung adenocarcinoma in the brain. Scale bar, 20 mm; × 400 magnification. A merged image is of TIAF1 (green), Aβ (red) and DAPI (blue). The blocking R48-2 peptide was used in negative controls. (c and d) In IHC staining, expression of WOX1 and TIAF1 is shown in neurofibromatosis NF1. TIAF1 is overexpressed in the peritumor area, but is less expressed in the tumor itself. Scale bar, 100 μm. (e) Fibrous protein aggregates are shown in the peritumor coats of neurofibromas. TIAF1 is present in the fibrous aggregates ( × 400 magnification). (f) R48-2 peptide was used to block the immunoreactivity in the negative control staining. (g) Metastatic 13-06-MG glioma cells were subcutaneously inoculated and shown to metastasize to the lung of a nude mouse. The growing tumor in the lung showed a high level expression of TIAF1 as compared with the normal lung of a nude mouse. Scale bar, 200 μm; × 400 magnification (IHC). A representative data set is shown from three repeats

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