Figure 5 | Cell Death & Disease

Figure 5

From: TRAIL (TNF-related apoptosis-inducing ligand) regulates adipocyte metabolism by caspase-mediated cleavage of PPARgamma

Figure 5

TRAIL-induced effects on adipocyte metabolism are caspase dependent. (a) SGBS adipocytes were incubated with 100 ng/ml TRAIL in the presence or absence of 10 μM zVAD.fmk. Glucose uptake was stimulated with 10−8 M insulin for 15 min. The cellular uptake of 14C-deoxy-glucose uptake was measured on a β-counter. Data are presented as mean+S.E.M. of four independent, triplicate experiments. *P<0.05 (zVAD+TRAIL versus TRAIL). (b) SGBS adipocytes were treated with 100 ng/ml TRAIL in the presence or absence of 10 μM zVAD. After 24 h, 10−8 M insulin and 14C-glucose was added for another 24 h. The incorporation of 14C-glucose into cellular lipids was measured. Data are presented as mean+S.E.M. of four independent, triplicate experiments. *P<0.05 (zVAD+TRAIL versus TRAIL). (c) SGBS adipocytes were incubated with 100 ng/ml TRAIL in the presence or absence of zVAD (10 μM) for 48 h. qPCR analysis was performed using primer pairs specific for Glut-4, ACC, and FASN. Data are represented as relative amount of mRNA expression normalized to GAPDH are presented as mean+S.E.M. of three independent experiments.*P<0.05 (TRAIL versus TRAIL+zVAD). (d) SGBS adipocytes were treated with 100 ng/ml TRAIL. Protein lysate were prepared after indicated time points and subjected to western blot analysis for caspases-1, -3, -6, and -8. One representative experiment out of three performed is shown

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