Figure 2

From: CD99 ligation upregulates HSP70 on acute lymphoblastic leukemia cells and concomitantly increases NK cytotoxicity

Figure 2

CD99 ligation induces HSP70 transcription in ALL but not in ET cells. (a) Treatment of ALL cells (Reh and Jurkat) with CD99 (DN16) mAb led to upregulation of hsp70 but not hsc70 mRNA expression. Representative picture of three experiments is shown (control – MOPC1-treated cells). (b) Western blot with anti-HSP70 antibody was performed after CD99 ligation by DN16 mAb in Reh and Jurkat cells. β-Actin was used as the loading control. (c) RT-PCR for constitutive hsc70 and inducible hsp70 was performed using TC252 and SKNMC cell lines. RNA input was normalized by equal number of non-apoptotic cells loaded per sample. (d) Corresponding FACS data showed no differences in HSP70 expression level in TC252 cells and slight downregulation of HSP70 on SKNMC cells (blue lines – MOPC1-treated cells, red lines – CD99 (DN16)-treated cells, black dashed lines – isotype control)