In the last decade, the endoplasmic reticulum (ER) has emerged as a central organelle regulating the core mitochondrial apoptosis pathway. At the ER membrane, a variety of stress signals are integrated toward determining cell fate, involving a complex cross talk between key homeostatic pathways including the unfolded protein response, autophagy, calcium signaling and mitochondrial bioenergetics. In this context, key regulators of cell death of the BCL-2 and TMBIM/BI-1 family of proteins have relevant functions as stress rheostats mediated by the formation of distinct protein complexes that regulate the switch between adaptive and proapoptotic phases under stress. Here, we overview recent advances on our molecular understanding of how the apoptotic machinery integrates stress signals toward cell fate decisions upstream of the mitochondrial gateway of death.
Mitochondrial outer membrane permeabilization (MOMP) is a critical check point in the control of apoptosis. MOMP is regulated by a balance between pro- and anti-apoptotic proteins of the BCL-2 family.
Under sustained endoplasmic reticulum (ER) stress conditions, the unfolded protein response (UPR) triggers apoptosis in part through the transcriptional and posttranslational regulation of proapoptotic BH3-only proteins.
Several proteins of the BCL-2 family localize to the ER membrane, impacting mitochondrial biology at a distance through the regulation of IP3R-dependent calcium release.
BAX and BAK homo-oligomerize at the ER membrane, leading to its permeabilization and the release of ER luminal proteins to the cytosol.
The BCL-2 protein family operates as a core to integrate stress signaling networks, regulating cell death, calcium homeostasis, the UPR and autophagy.
How does ER permeabilization lead to cell death?
What is the functional relationship between proteins of the BCL-2 and TMBIM families in the regulation of cell death?
Are TMBIM proteins ion channels? Is the channel activity relevant for their apoptotic regulatory functions?
Do BCL-2-related proteins modulate the switch between proadaptive to proapoptotic ER stress responses?
Apoptosis is a highly conserved and tightly regulated process to eliminate damaged or unrequired cells. Alterations in the control of apoptosis are at the root of a large array of human diseases, including cancer, neurodegenerative diseases and autoimmunity, among others.1, 2 Cell death driven by apoptosis is characterized by a specific sequence of morphological changes,3 starting with cell shrinkage, chromatin and organelle fragmentation, and loss of cell-to-cell and cell-to-matrix interactions, followed by the disintegration of the cell into apoptotic bodies and their subsequent engulfment and degradation by phagocytic cells.4 These phenotypic changes are orchestrated by specialized cysteine–aspartic proteases of the caspase family, which cleave and inactivate more than 400 intracellular protein targets, catalyzing the controlled demolition of the cell.4
In vertebrates, apoptosis proceeds through two distinct but interrelated signaling cascades defined as the intrinsic and extrinsic cell death pathways. In the extrinsic pathway, the engagement of membrane receptors (i.e., TNFα or Fas receptors) results in the recruitment of distinct adapter proteins and activation of initiator caspase (i.e., caspase-8), which in turn cleaves and activates executioner caspases (i.e., caspase-3 and -7). The intrinsic pathway of apoptosis is initiated in response to a myriad of intracellular physiological or cytotoxic stimuli, such as DNA damage, viral infections and endoplasmic reticulum (ER) stress, among others.5 Even though these perturbations can be initiated at different cell sites, most of the signaling pathways triggered by irreversible damage converge at the mitochondria outer membrane, where they trigger its permeabilization.5
Mitochondria outer membrane permeabilization (MOMP) is considered the point of no return of the intrinsic apoptosis pathway and thus, a critical check point for cell death control. MOMP results in the release of cytochrome c and many other proapoptotic factors, leading to the formation of the apoptosome followed by the activation of the caspase cascade.1, 2 Given its central role in apoptosis, MOMP is tightly controlled by several positive and negative regulators, where we highlight the involvement of the B-cell lymphoma 2 (BCL-2) family of proteins. In mammals, the BCL-2 family is composed by at least 20 members, functionally grouped into anti- and proapoptotic proteins, and further subdivided according to the presence of up to four BCL-2 homology (BH) domains1, 6 (Figure 1a). Anti-apoptotic proteins contain all four BH domains and include BCL-2 itself, BCL-XL, MCL-1, BCL-w and A1. Proapoptotic proteins are further subdivided into two groups: multidomain proteins BAX, BAK and BOK, also containing all four BH domains and ‘BH3-only members’ (i.e., BID, BIM, NOXA and BAD); evolutionary divergent proteins that only bear the BH3 domain operating as essential apoptosis inducers.1, 6
The BCL-2 family may be viewed as a tripartite apoptotic switch,7 where BAX and BAK act as gatekeepers of MOMP (Figure 1b). Indeed, apoptosis signals trigger the translocation of BAX from the cytosol to the mitochondria, where it assembles into higher-order homo-oligomers that form a pore that permeabilize the mitochondrial outer membrane.7 BAX and BAK activation and oligomerization is engaged by upstream BH3-only proteins and antagonized by anti-apoptotic BCL-2 family members; either by direct interaction or inhibition of BH3-only proteins (Figures 1b and 4a). Thus, the balance between anti- and proapoptotic proteins determines the survival or death of a cell.1, 6 Although the primary place of action of the BCL-2 family is at the mitochondrial membrane, several members also localize to the ER in different proportions, where they can modulate the mitochondrial apoptosis pathway at a distance by several mechanisms.8 Indeed, in the last decade, the ER has emerged as a critical place of apoptosis control in response to a wide variety of stress stimuli. This review focuses on discussing the contribution of the BCL-2 family to various stress responses at the ER membrane and the consequences over the intrinsic cell death pathway.
ER stress signaling and cell fate control
The ER is the main organelle involved in protein folding and secretion where around a third of the total proteome is synthetized; in addition to operate as the central intracellular calcium store and a site of lipid synthesis. Several perturbations can result in ER stress, a cellular condition involving the accumulation of unfolded or misfolded proteins in its lumen.9 ER stress engages a complex and dynamic signaling network termed the unfolded protein response (UPR). The UPR operates as a global rheostat to determine cell fate under ER stress. The UPR initially signals to establish an adaptive program aimed at reestablishing ER proteostasis by increasing the folding capacity of the cell, reducing protein synthesis and enhancing the clearance of abnormally folded proteins and damaged organelles.10 If ER homeostasis cannot be restored, the UPR shifts its signaling toward a proapoptotic response to eliminate the damaged cell.11 Here we summarize key aspects of UPR signaling involved in cell survival and apoptosis control and how it relates to the BCL-2 family of proteins.
The UPR involves a complex signaling transduction pathway, initiated by at least three type of stress sensors or transducers localized to the ER membrane, including inositol-requiring enzyme 1 (IRE1)α, PKR-like endoplasmic reticulum kinase (PERK) and activating factor 6 (ATF6)α.12 IRE1α is a bifunctional kinase and endoribonuclease enzyme representing the most conserved UPR signaling branch. Under ER stress, IRE1α becomes activated and mediates the expression of the transcription factor X-box binding protein 1 (XBP1),9 controlling genes involved in ER homeostasis maintenance.12 The RNase domain of IRE1α has additional substrates, degrading many RNAs through a process known as regulated IRE1-dependent decay (RIDD).13 Upon activation, PERK signaling blocks protein synthesis by phosphorylating the translation initiator factor eIF2α, reducing the overload of misfolded proteins inside the ER. eIF2α phosphorylation allows the selective translation of ATF4, a transcription factor that transactivates genes related to protein folding, redox control and amino-acid metabolism.14 Under ER stress, ATF6 translocates to the Golgi apparatus where it is processed to release an active transcription factor contained on its cytosolic domain that upregulates UPR target genes related to stress mitigation.
ER stress-mediated apoptosis
Under acute or prolonged ER stress, the UPR activates a complex cell death program mediated by several components.15 This regulation is achieved by the transcriptional and posttranslational modulation of several components of the BCL-2 family that engage the core mitochondrial apoptosis pathway15 (Figure 2). Under chronic ER stress, ATF4 contributes to apoptosis by upregulating the transcription factor C/EBP homologous protein (CHOP, also known as GADD153),16, 17 and by enhancing oxidative stress and protein synthesis.18, 19, 20
Under sustained ER stress, IRE1α is turned off in certain cellular systems,21, 22 which may attenuate the prosurvival effects of XBP1 expression and upregulate the death receptor DR5, engaging caspase-8 and BID-dependent cell death.23 Additionally, the activation of BID by caspase-2 has also been proposed to contribute to ER stress-mediated cell death through the direct control of miRNAs by IRE1α’s RIDD activity24, 25 (Figure 2), although in some cellular systems caspase-2 was dispensable for ER stress-mediated apoptosis.26 IRE1α may also elicit apoptosis through the activation of JNK.27, 28 JNK may have an indirect effect on the mitochondria by fine tuning the affinity of BCL-2 to BH3-only proteins or by activating proteins like BIM.29, 30
BH3-only proteins are essential to induce ER stress-mediated cell death. In fact, triple-deficient cells for the BH3-only proteins BIM, PUMA and BID are highly resistant to ER stress,31 similar to BAX and BAK double-deficient cells32, 33 and animals,34 indicating a key role of the core proapoptotic program in the induction of apoptosis by ER-related injuries. Several BH3-only proteins are induced under acute ER stress including the BH3-only proteins PUMA, NOXA, BAD and BIM.8 PUMA and NOXA are transcriptionally upregulated by p53 under ER stress,35 whereas BIM can be regulated by phosphorylation or transcriptionally mediated by the UPR transcription factor CHOP,30 in addition to be modulated by miRNAs in an ATF4-dependent manner.36 Overall, the exact impact of specific BH3-only proteins to apoptosis under ER stress appears to be cell-type and stimuli-dependent.
BOK is a poorly studied BCL-2 family member with extensive amino-acid sequence similarity to BAX and BAK, but with a restrictive expression pattern.37, 38, 39, 40 Deletion of BOK had no effects on mouse development, and cells derived from these animals did not exhibit any defect in cell death induction, suggesting BOK is dispensable for apoptosis.41, 42, 43 Nevertheless, recent studies have found that BOK localizes mainly to the ER membrane, where it contributes to apoptosis in response to ER stress.40, 44 Moreover, recent work has indicated that BOK is constitutively active and directly triggers MOMP-independently of BAX and BAK.45, 46 Under normal conditions, BOK is continually degraded by the proteasome through the ER-associated degradation (ERAD) pathway. Interestingly, if ERAD and the UPR are altered, BOK is stabilized, translocating to the mitochondria to induce MOMP.45 Thus, BOK may function as a direct apoptotic switch in conditions of chronic ER stress.
Taken together, these studies suggests that under irreversible ER stress, UPR signals engage a cluster of BH3-only proteins, serving as a molecular cross talk between the ER and mitochondria to trigger cell destruction (Figure 2). Overall, the activation of apoptosis by ER stress is complex and does not depend on a unique mechanism (reviewed in Tabas and Ron11). A combination of different signaling pathways integrates information at the ER membrane about intensity and duration of the stress toward the activation of BAX, BAK and BOK (Figure 2) (see next sections).
The BCL-2 family and the regulation of ER calcium homeostasis
The regulation of calcium release from the ER is a relevant modulatory step in determining cell fate in response to a myriad of physiologic and cytotoxic stimuli. Calcium is released from the ER primarily through ER membrane-associated inositol 1,4,5-trisphosphate (IP3)-gated channels, also called IP3 receptors (IP3Rs). Interestingly, IP3Rs are distributed in specialized ER subdomains – denominated mitochondria ER-associated membranes (MAMs) – localized in close proximity to the mitochondria.47 MAMs facilitate fast and localized calcium transfer, establishing an inter-organelle coupling with important implications for cytosolic calcium buffering, mitochondria metabolism and cell death.47, 48
The cellular outcome of a rise in cytosolic calcium concentration and its subsequent uptake by the mitochondria depends on the intensity, duration and pattern of the calcium signal itself, and the presence or absence of relevant regulatory proteins. For example, high-frequency cytosolic calcium oscillations or bursts are efficiently transmitted to the mitochondria, resulting in the activation of several calcium-dependent mitochondrial enzymes, the F-ATP synthase itself and metabolite carriers, leading to increased mitochondrial respiration and ATP production.48, 49 On the other hand, sustained calcium release from the ER may overload the mitochondrial matrix triggering a BAX- and BAK-independent mechanism of mitochondrial permeabilization and cytochrome c release known as the mitochondria permeability transition pore (mPTP).50, 51, 52 The mPTP is associated with the expansion of the mitochondrial matrix, triggering sufficient swelling to rupture the inner and outer mitochondrial membranes, causing the release of apoptotic mediators, including cytochrome c, the dissipation of the mitochondrial membrane potential (Δψm) and a sudden decrease of ATP levels.53, 54 The opening of the mPTP is associated with a generalized mitochondrial metabolic dysfunction and necrotic cell death.50 Although the molecular identity of the mPTP remains controversial,50 different components of the pathway have been proposed, where we highlight Cyclophilin D (CypD) as a major regulator53, 54, 55, 56 (Figure 3). Of note, evidence supporting the existence of a putative BAX/BAK- and CypD-independent pore for cytochrome c release and apoptosis has been provided under conditions of ER stress or calcium unbalance.57, 58 In this section, we overview a set of reports defining the contribution of the BCL-2 family to the control of ER calcium homeostasis and its impact in mitochondrial biology.
The vast majority of the studies support a role of the BCL-2 family in the control of steady-state ER calcium content and stimulated calcium release.49, 59, 60 In general terms, proapoptotic BCL-2 members promote sustained calcium release engaging the release of cytochrome c, while anti-apoptotic BCL-2 proteins exhibit a dual function; inhibiting proapoptotic calcium release and promoting prosurvival oscillatory calcium signaling (reviewed in Rong and Distelhorst49). Accumulating evidence indicates that the molecular mechanism underlying the control of ER calcium homeostasis by BCL-2 proteins is mediated in part by the IP3Rs where two major mechanisms are proposed (Figure 3, left panel). First, BCL-2 interacts with the central modulatory and coupling domain of the IP3R through the BH4 domain.59, 61, 62 The functional consequence of this interaction is the inhibition of IP3-mediated calcium release and IP3R-induced apoptosis.61, 62 Second, BCL-2, BCL-XL and MCL-1 directly interact with the C-terminal domain of all three IP3R isoforms.63, 64, 65, 66 In contrast to BCL-2-mediated inhibition of IP3R, these interactions sensitize IP3Rs to low levels of IP3, inducing a high-frequency oscillatory calcium release pattern, which is correlated with increased mitochondrial bioenergetics and resistance to cell death.63, 64, 65 BCL-2 may also modulate the activity of ryanodine receptors.67
A fraction of the cellular pool of BAX and BAK resides in the ER membrane.68, 69, 70 BAX or BAK overexpression causes the rapid depletion of ER calcium stores, resulting in increased mitochondrial calcium uptake and cytochrome c release, probably triggering the mPTP.68, 69, 70 Indeed, restoring the ER calcium content deficit in BAX/BAK double knockout cells by overexpressing the SERCA pump reestablished cell death by mPTP-inducing stimuli, but not by BH3-only proteins, dissecting the contribution of BCL-2 proteins to mPTP and MOMP.52 The exact mechanism by which BAX and BAK induce apoptosis from the ER is not well understood. One hypothesis suggests that BAX and BAK activation at the ER membrane results in increased ER-to-mitochondria calcium transfer, leading to the opening of mPTP and necrotic cell death.52 As an alternative, an increase in cytosolic calcium can also lead to Calcineurin activation in the cytosol, indirectly engaging MOMP through the activation of the BH3-only protein BAD.71 In addition, the activation of ER stress signals from the ER on a BAX- and BAK-dependent manner may also contribute to apoptosis (see next sections). Several BH3-only proteins also localize to the ER where they modulate ER calcium homeostasis including BIK and PUMA.35, 72, 73, 74 Moreover, the physical association of BCL-XL with the IP3R can be displaced by BAX or BH3-only proteins, illustrating a model where several proteins of the BCL-2 family converge at the ER membrane to regulate IP3R dynamics, fine tuning mitochondrial function and the susceptibility of cells to undergo apoptosis. Overall, many other studies have linked the activity of BH3-only proteins to ER calcium regulation through the formation of distinct protein complexes with BCL-2 family members at the ER membrane, with a similar biochemical profile to the mitochondrial pool (Figures 3 and 4b).
Regulation of ER permeability by BAX and BAK
Early reports indicated that different BCL-2 family members have channel properties with structural similarity to pore-forming bacterial toxins.75 Indeed, the pore activity of BAX is essential to trigger apoptosis and cytochrome c release, as demonstrated in vitro and in mouse models using small molecule inhibitors that selectively block the channel pore.76 Electrophysiological studies have determined that BAX lacks specific conductivity for bivalent cations.77 Nevertheless, BAX and BAK form higher-order structures that are permeable to large proteins and dextrans (up to 2 MDa).78, 79, 80 Indeed, using nanoscale super-resolution microscopy, two recent studies have shown BAX forms ring- and arc-like aggregates in the MOM after apoptosis induction, consistent with the presence of an oligomeric proteinaceous pore.81, 82 These ring-like structures have a diameter ranging from 50 to 700 nm, theoretically allowing the release of large proteins and dextrans, as previously discussed.81, 82
A few studies suggest that BAX and BAK may also activate and assemble into higher-order oligomers at the ER membrane in response to stress.70, 83, 84 Furthermore, enforced expression of BH3-only proteins at the ER membrane causes BAX oligomerization at the ER membrane, a phenomenon inhibited by BCL-273, 84 (Figure 3). The ER membrane itself may become permeabilized under ER stress conditions, a phenomena regulated by the BCL-2 family.83 ER luminal chaperones including Grp78/BiP, Grp94, calreticulin and PDI are released into the cytosol in a BAX- and BAK-dependent manner. This process is initiated by upstream BH3-only proteins and antagonized by BCL-XL and BCL-2.83, 85 Although the role of the released protein in apoptosis is not clear, another report suggested that the translocation of certain disulfide isomerases (i.e., PDI) to the ER-mitochondrial contact sites contribute to cell death in models of neurodegeneration.86 Thus, the biochemical interactions regulating this putative ER permeabilization resemble the hierarchical control of MOMP. Interestingly, it was recently found that the UPR sensor IRE1α plays a critical role in the regulation of ER membrane permeabilization under ER stress, a phenomenon mediated by the BH3-only protein BNip3.85
Although the significance of ER permeabilization to cell death needs to be further determined, we speculate that this process may (i) induce a massive release of ER calcium engaging mPTP or calcium-dependent cytosolic enzymes (e.g., Calcineurin), (ii) deplete the ER of essential folding components, exacerbating ER stress and affecting protein trafficking and (iii) enhance ROS production through altered ER redox and mitochondrial bioenergetics.
Additional players in ER-to-mitochondria cross talk: BAX inhibitor 1 and the TMBIM family
In addition to the BCL-2 family, several other proteins are involved in the regulation of ER calcium dynamics and apoptosis at the ER membrane. The transmembrane BAX inhibitor motif containing (TMBIM) family is composed of at least six highly conserved members that play a critical role in apoptosis and calcium regulation in response to several cell death stimuli.87 The TMBIM family of proteins is highly conserved throughout evolution, with homologs in species where no BCL-2 members have been described, including bacteria, yeast and plants.87, 88 TMBIM family members are characterized by the presence of up to seven transmembrane domains and the UPF0005 motif of unknown function, and includes the following members: centrifugal force and shear stress (RECS1/TMBIM1), lifeguard (LFG/TMBIM2), glutamate receptor ionotropic NMDA-associated protein 1 (GRINA/TMBIM3), Golgi anti-apoptotic protein (GAAP/TMBIM4), growth hormone inducible transmembrane protein (GHITM/TMBIM5) and BAX inhibitor 1 (BI-1/TMBIM6).87 All TMBIM family members are implicated in apoptosis inhibition by different mechanisms.87 The crystal structure of the Bacillus subtilis TMBIM homolog BsYetJ was solved, revealing a seven transmembrane pH-sensitive calcium channel.89 Cation conductivity has been demonstrated for the human homologs BI-1 and GAAP, proposing a general mechanism for the entire TMBIM family in the regulation of cell death.90
BI-1 is the founder member of the family, first identified in yeast screenings for inhibitors of cell death triggered by BAX overexpression.91 We reported a functional relationship between components of the TMBIM family, observing that BI-1 and GRINA control cell death under ER stress conditions, having synergistic and complementary activities in the regulation of apoptosis.92, 93 BI-1 and GRINA physically interact, and are embedded within the ER membrane where they specifically inhibit cell death triggered by ER stress, possibly through the modulation of ER calcium dynamics by interactions with the IP3Rs.92, 93, 94, 95 BI-1 may also modulate apoptosis in part through the induction of mPTP.96 Thus, members of the TMBIM family regulate cell death indirectly, possibly through the modulation of calcium signaling across different intracellular membranes. It remains to be determined if TMBIM family members antagonize BH3-only proteins or alter the activity of multidomain BCL-2 family members.
What is the relationship between the TMBIM and the BCL-2 protein family? BI-1 is required for the regulation of ER calcium content downstream of the BCL-2 family.95 BI-1 and GRINA physically interact with BCL-2 and BCL-XL,21, 92, 93 and the BH4 motifs of these proteins enhance BI-1 oligomerization and calcium efflux.97 Since BI-1 has recently been identified as a pH-sensitive calcium leak channel, an interesting hypothesis is that BCL-2 family members regulate TMBIM channel gating properties by direct interactions, in a manner analogous to their regulation of IP3Rs.89 Further studies are needed to fully elucidate the role of BCL-2-related proteins in the regulation of calcium dynamics and its possible connection with the TMBIM family of proteins.
Stress signal integration at the ER membrane by the BCL-2 family
In the last decade, the ER membrane has emerged as a hub where different stress signals are integrated to determine cell fate decisions.5, 98 Accumulating evidence suggest a new concept where members of the BCL-2 families operate as stress signal integration platforms through physical interactions that control the activity of key core components of distinct stress pathways (Figure 4). At the molecular level, a remarkable triade is emerging where the apoptosis, UPR and autophagy machineries converge into interrelated protein complexes at the ER membrane through interactions with BCL-2 family members, affecting global proteostasis in addition to mitochondrial bioenergetics. In this section we summarize a few examples depicting the cross talk between key apoptosis regulatory proteins and central stress pathways to modulate adaptation processes or cell demise
Fine tuning the UPR by the BCL-2 family
Accumulating studies indicate that a variety of apoptosis-related proteins physically interact with the ER stress sensor IRE1α, regulating the threshold to engage UPR responses.12 IRE1α is proposed to operate as a scaffold, where many regulators assemble to modulate ER stress signaling and the cross talk with diverse stress pathways through a platform referred to as the UPRosome.99 We initially reported that BAX and BAK instigate UPR responses (i.e., JNK and XBP1).34 In certain experimental systems, BH3-only proteins such as BIM and PUMA can enhance IRE1α activation through a physical interaction.100 Unexpectedly, the expression of BCL-2 was required for this positive regulation, whereas BAD or the BH3 mimetic ABT-737 antagonized this pathway,100 suggesting that the basal antagonized complex operates to control the UPR (Figure 4c). Alternatively, BCL-2 may modulate global ER stress signaling through unknown mechanisms.101 BI-1 has a relevant activity in attenuating IRE1α signaling under prolonged ER stress also involving the formation of a protein complex that competes for BAX.21, 102, 103 Many other apoptosis-related proteins also associate with IRE1α,12, 99 supporting the concept that apoptosis-related proteins have an alternative role at the ER, modulating the threshold to induce or attenuate ER stress (Figure 4c).
Regulation of macroautophagy by the BCL-2 family
The BCL-2 family also has relevant activities at the ER to control the levels of autophagy, an adaptive response that has a prosurvival function under nutrient starvation but also ER stress.104 However, overactivation of autophagy may be detrimental.105 There is a coordinated regulatory cross talk between the UPR (early stages) and the induction of autophagy. Autophagy is regulated at the ER membrane through the sequestration and consequent inhibition of Beclin-1 – one of the key players in autophagy induction – by anti-apoptotic members of the BCL-2 family.106, 107, 108, 109 Beclin-1 carries a BH3-like motif and interacts with the groove of BCL-2, BCL-XL and MCL-1 in an analogous manner as BH3-only proteins107, 108, 109 (Figure 4d). However, Beclin-1 does not have a proapoptotic activity.110 In addition, the interaction between Beclin-1 and BCL-2 can be modulated by BH3-only proteins and BH3 mimetics like ABT-737.108, 109 Upon amino-acid starvation or ER stress, BCL-2 becomes phosphorylated within its N-terminal unstructured loop, disrupting its interaction with Beclin-1 and resulting in autophagy induction.111, 112 BH3-only proteins also enhance autophagy by disrupting the Beclin-1/BCL-2 interaction.107, 111, 112, 113 Although several laboratories have provided evidence supporting the connection between Beclin-1 and BCL-2 family members, recent studies have challenged this model, proposing an alternative mechanism whereby prosurvival BCL-2 proteins inhibit autophagy only indirectly, through their interaction with and sequestration of proapoptotic BAX and BAK.114, 115 However, another study indicated that BAX and BAK are dispensable for ABT-737-induced autophagy.116 Further studies are necessary to precisely determine the functional interplay between the BCL-2 family and components of the autophagy machinery.
BI-1 has a relevant activity in regulating autophagy through its repressor activity on the IRE1α/JNK pathway, affecting the phosphorylation of the BCL-XL/Beclin-1 complex at the ER.117 Besides, BI-1 may also enhance autophagy under certain conditions through the release of calcium and activation of AMP kinase.118, 119 Although a similar activity has been reported for the IP3Rs,120 BCL-2 and BCL-XL have a clear inhibitory activity on autophagy through Beclin-1, similar to BI-1.117 Thus, the interplay between members of the BCL-2 and TMBIM families at the ER membrane integrates stress responses through the formation of distinct protein complexes, in addition to modulate ER calcium homeostasis and its metabolic control with the mitochondria.
Here we have overviewed emerging evidence supporting a complex interplay between the BCL-2 family of proteins at the ER with several factors involved in stress signaling and the control of mitochondrial-dependent apoptosis. Dynamic protein complexes are assembled at the ER membrane to fine tune and integrate multiple stress responses that set the global threshold for apoptosis induction. At the center of this regulation, the BCL-2 and TMBIM families play a key role in the cross talk and integration of multiple signaling pathways that control the maintenance of global proteostasis and mitochondrial bioenergetics. Accordingly, beyond the regulation of MOMP, BCL-2- and TMBIM-related proteins have distinct activities at the ER membrane to control ER calcium dynamics, UPR activation, autophagy levels and ER permeabilization. In general terms, multiple bidirectional regulation points can be identified, reflecting the dynamic nature of interactions that orchestrate global outputs in response to cellular and environmental stress to engage adaptive programs, mitochondrial bioenergetics or cell death (Figure 5).
Given the existence of diverse novel functions of the BCL-2 and TMBIM families in physiological processes beyond apoptosis, it has been suggested that these proteins act as specialized ‘stress sentinels’, operating as molecular switches to transit between prosurvival responses and the induction of the core apoptosis pathway in response to irreversible stress.8 Since several small molecules have been designed to target BCL-2 family members for the treatment of cancer,7 it is essential to consider possible side effects of targeting the core apoptosis pathways in other-related processes including ER-related functions.
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This work was funded by FONDECYT no. 3150113 (AC), FONDECYT no. 1140549, FONDAP program 15150012, Millennium Institute P09-015-F, European Commission R&D MSCA-RISE #734749 (CH). We also thank the support from Michael J Fox Foundation for Parkinson's Research – Target Validation grant No 9277, FONDEF ID16I10223, FONDEF D11E1007, US Office of Naval Research-Global (ONR-G) N62909-16-1-2003, U.S. Air Force Office of Scientific Research FA9550-16-1-0384, ALSRP Therapeutic Idea Award AL150111, Muscular Dystrophy Association 382453 and CONICYT-Brazil 441921/2016-7 (CH). PP is a postgraduate fellow supported by a CONICYT fellowship.
The authors declare no conflict of interest.
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Pihán, P., Carreras-Sureda, A. & Hetz, C. BCL-2 family: integrating stress responses at the ER to control cell demise. Cell Death Differ 24, 1478–1487 (2017). https://doi.org/10.1038/cdd.2017.82
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