Figure 7 | Cell Death & Differentiation

Figure 7

From: Functional regulation of FoxO1 in neural stem cell differentiation

Figure 7

miR-9 regulates Notch pathway through FoxO1. (a) CSL-induced luciferase reporter activity was measured in control or miR-9-overexpressed NSPCs. (b) Same experiment as in (a) was performed in wild-type (Ade-emp) or FoxO1-knockout (Ade-Cre) NSPCs. (c) Quantitative real-time PCR (qRT-PCR) analysis of βIII-tubulin and Hes1 mRNA in Ade-emp- or Ade-FoxO1-infected NSPCs, with or without miR-9 overexpression. (d) The FoxO1 occupancy of the Hes1 promoter in NSPCs was determined by chromatin immunoprecipitation (ChIP). (Upper) Predicted FoxO1 binding in the Hes1 promoter. TSS: transcription start site. (Lower) qPCR against two regions in the Hes1 promoter. (e) Western blot analysis of βIII-tubulin and Hes1 expression in Ade-empty- or Ade-Cre-infected FoxO1L/L NSPCs, with (D) or without (V) treatment of 5 μM DAPT. (f) qRT-PCR analysis of βIII-tubulin and Hes1 mRNA in Ade-empty- or Ade-Cre-infected FoxO1L/L NSPCs, with or without DAPT treatment. *P<0.05 and **P<0.01. (g) A graphical presentation of miR-9 and FoxO1 interaction on Notch target gene regulation

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