Figure 1 | Cell Death & Differentiation

Figure 1

From: Functional regulation of FoxO1 in neural stem cell differentiation

Figure 1

miR-9 promotes neuronal differentiation of adult NSPCs. (a) miR-9 sponge or miR-9-encoding virus-infected cells were induced to differentiate for 3 days, and they were immunostained with a βIII-tubulin-specific antibody (red). GFP-expressing retrovirus was used as a control. Nuclear DAPI (4',6-diamidino-2-phenylindole) staining is shown in blue. Scale bar=200 μm. (b) The quantification of βIII-tubulin-positive cell proportions in control, miR-9-overexpressing, miR-9 sponge-expressing NSPCs as in (a). (c) Western blot analysis of βIII-tubulin expression in control, miR-9 sponge or miR-9-encoding virus-infected neural stem cells. β-Actin was used as a loading control. (d) The mRNA expression of βIII-tubulin in control, miR-9-overexpressing, miR-9 sponge-expressing NSPCs, as measured by real-time RT-PCR analysis. The mRNA level of control cells was set to 1. *P<0.05. Representative results from multiple experiments are shown

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