Abstract
Erythropoiesis is a tightly regulated process in which multipotential hematopoietic stem cells produce mature red blood cells. Here we show that deletion of poly(ADP-ribose) polymerase-2 (PARP-2) in mice leads to chronic anemia at steady state, despite increased erythropoietin plasma levels, a phenomenon not observed in mice lacking PARP-1. Loss of PARP-2 causes shortened lifespan of erythrocytes and impaired differentiation of erythroid progenitors. In erythroblasts, PARP-2 deficiency triggers replicative stress, as indicated by the presence of micronuclei, the accumulation of γ-H2AX (phospho-histone H2AX) in S-phase cells and constitutive CHK1 and replication protein A phosphorylation. Transcriptome analyses revealed the activation of the p53-dependent DNA-damage response pathways in PARP-2-deficient cells, culminating in the upregulation of cell-cycle and cell death regulators, concomitant with G2/M arrest and apoptosis. Strikingly, while loss of the proapoptotic p53 target gene Puma restored hematocrit levels in the PARP-2-deficient mice, loss of the cell-cycle regulator and CDK inhibitor p21 leads to perinatal death by exacerbating impaired fetal liver erythropoiesis in PARP-2-deficient embryos. Although the anemia displayed by PARP-2-deficient mice is compatible with life, mice die rapidly when exposed to stress-induced enhanced hemolysis. Our results pinpoint an essential role for PARP-2 in erythropoiesis by limiting replicative stress that becomes essential in the absence of p21 and in the context of enhanced hemolysis, highlighting the potential effect that might arise from the design and use of PARP inhibitors that specifically inactivate PARP proteins.
Similar content being viewed by others
Main
Erythropoiesis is a complex multistage process in which multipotential hematopoietic stem cells give rise to mature red blood cells (RBCs) through coordinated programs of gene expression, proliferation and differentiation.1 The earliest committed erythroid progenitor is the erythroid burst-forming unit (BFU-E) that further differentiates through erythroid colony-forming unit (CFU-E), proerythroblasts, basophilic and polychromatophilic erythroblasts and orthochromatic erythroblasts, giving rise to reticulocytes by enucleation. Reticulocytes are then released into the circulation, where they shed their RNA and degrade mitochondria to become mature RBCs.2, 3 Erythropoiesis must be tightly regulated to maintain the production of RBCs under both normal and stress conditions. This complex process is controlled intricately at each stage of the production of RBCs by multiple proteins, RNAs, DNA and chromatin modifications.1, 4, 5, 6, 7 Impairment of these regulatory pathways can lead to anemia, which has been estimated to affect nearly a quarter of the world's population.8 Moreover, defective erythropoiesis can be fatal under conditions of stress such as acute blood loss, chronic infections and myeloablative therapeutic procedures used to treat malignant diseases. Therefore, a better understanding of the molecular mechanisms controlling the production of RBCs are important to control anemia and may lead to avenues for developing new therapeutic strategies.
Poly(ADP-ribose) polymerase-2 (PARP-2) belongs to a family of enzymes that catalytically cleave β-nicotinamide adenine dinucleotide (β-NAD+) and transfer the ADP-ribose moiety to acceptor residues of target proteins, modifying their functional properties by creating long chains of poly(ADP-ribose).9, 10 This modification is a dynamic process, as indicated by the short half-life of the ADP-ribose polymer, which is rapidly subjected to degradation by the poly(ADP-ribose)-glycohydrolase and ADP-ribosyl-hydrolases.9 Among the PARP proteins, PARP-1, PARP-2 and PARP-3 enzymatic activities are stimulated by DNA strand interruptions targeting mainly proteins involved in chromatin structure, DNA metabolism and repair, suggesting a role for these proteins in the cellular response to DNA damage and maintenance of genomic stability.9, 11, 12 Accordingly, PARP inhibitors that compete with β-NAD+ at the highly conserved enzyme's active site arise as new potential therapeutic drugs used for chemo- and radiopotentiation and for the treatment of cancers with specific DNA repair defects where they can trigger synthetic lethality as single agents.13 Despite increasing biochemical and structural evidences suggesting specific roles for these proteins in the DNA-damage response, PARP inhibitors currently in clinical trials are still unable to discriminate individual PARP-isoforms.14 For instance, PARP-2 can become selectively activated by specific stimuli,15 has particular targets and/or interacts with specific protein partners,16, 17, 18 suggesting functions independent of PARP-1 that have started to emerge. Notably, PARP-2, but not PARP-1, has been shown to be essential for T-cell development and genomic stability in thymocytes,19, 20 spermatogenesis,21 suppression of activation-induced cytidine deaminase (AID)-induced IgH/c-myc translocation,22 adipocyte differentiation,23 SIRT1 expression,24 cholesterol homeostasis25 and in the DNA-damage response of hematopoietic stem/progenitor cells, preserving hematopoiesis under conditions of irradiation stress.26 Understanding the role of individual PARP proteins in physiology and under pathological conditions will provide invaluable clues to design new therapeutic approaches targeting this family of enzymes.
Here, we show that deficiency of PARP-2, but not of PARP-1, in mice leads to chronic anemia at steady-state conditions. Loss of PARP-2 results in a decrease in RBC count, enlarged erythrocyte size, the presence of Howell–Jolly bodies and a reduced lifespan of circulating erythrocytes. Our results reveal that this deficiency is associated with an accumulation of replicative stress (RS) in erythroid progenitors, which limits their expansion through the activation of cell-cycle checkpoints and apoptotic mediators. Finally, we show that this protective function of PARP-2 becomes essential in the absence of p21 and in the context of enhanced hemolysis. Our findings establish a pivotal, nonredundant role of PARP-2 in erythropoiesis that might impact on the development of PARP-centered therapies.
Results
Loss of PARP-2, but not loss of PARP-1, causes anemia in mice
Hematological analysis of PARP-2-deficient (Parp-2−/−) mice at steady-state conditions revealed anemia as shown by a significant decrease in the number of RBCs, hematocrit and hemoglobin levels compared with wild-type littermate controls (Figures 1a–c). The mean corpuscular hemoglobin (MCH) and the mean concentration of corpuscular hemoglobin (MCHC) were similar in wild-type and Parp-2−/− mice (Figures 1d and e), indicating that the reduction in hemoglobin levels detected in Parp-2−/− mice was caused by a decrease in RBC counts. We also observed a considerable increase in the mean corpuscular volume (MCV) of RBCs from Parp-2−/− compared with wild-type mice (Figure 1f). Consistently, flow cytometry data showed significant increased forward light scatter in Parp-2−/− RBCs, providing additional evidence for increased RBC size in Parp-2−/− mice (Figure 1g). Noteworthy, the anemia phenotype was not observed in Parp-1−/− mice (Figure 1). Moreover, this phenotype was restricted to the erythroid compartment as white blood cell and platelet counts were normal in Parp-2−/− and Parp-1−/− mice (Supplementary Figure S1). Altogether, these data indicate that PARP-2, but not PARP-1, deficiency in mice leads to anemia despite normal serum iron levels (Figure 1h).
Anemia is known to trigger a compensatory response through the increase of erythropoietin (EPO) levels in plasma. The raised EPO promotes recovery from anemia by acting on erythroid progenitors in bone marrow (BM) and spleen, stimulating them to increase the rate at which RBCs are generated.27 Accordingly, elevated EPO levels are often associated with an increased number of circulating reticulocytes, which allows an assessment of erythropoietic rate. In agreement with this, we found an increase in EPO plasma levels in Parp-2−/− mice (Figure 1i), which associated with a 2.6-fold increase in their reticulocyte index (Figure 1j), reflecting the degree of compensatory response of Parp-2−/− hematopoietic tissues to anemia. However, the compensatory EPO response and the ensuing increase in circulating reticulocytes, which might account for the increased RBC size, are insufficient to avoid anemia in Parp-2−/− mice.
Shortened RBC lifespan in PARP-2-deficient mice
The anemia observed in Parp-2−/− mice could be due to increased RBC destruction, faulty RBC production or a combination of both processes. To determine the RBC lifespan in vivo, we monitored the disappearance of biotinylated RBCs at different time points. RBCs circulate for about 40 days in mice until they are taken up by splenic macrophages.28 Consistently, the time required for 50% of labeled erythrocytes to be lost from wild-type mice was 19 days. However, the half-life of labeled erythrocytes decreased to 13 days in Parp-2−/− mice (Figure 2a and Supplementary Figure S2). Hence, the absence of PARP-2 causes significant premature loss of circulating RBCs. In agreement, Perls-stained spleen sections from Parp-2−/− mice showed a marked increase in iron deposits compared with the wild-type mice (Figure 2b). Increased extravascular hemolysis of RBCs in PARP-2-deficient mice is also revealed by decreased serum haptoglobin, increased serum indirect bilirubin and normal serum hemoglobin (Figure 2c). The excessive removal of Parp-2−/− RBCs might imply that they are damaged.29, 30 Indeed, analysis of peripheral blood smears revealed a striking accumulation of micronuclei (Howell–Jolly bodies) on PARP-2-deficient RBCs (12.5%) (Figure 2d). The presence of Howell–Jolly bodies in PARP-2-deficient RBCs is consistent with the segregation defects that arise in response to RS.31, 32, 33 Thus, defective erythropoiesis, which might be due to the accumulation of RS on erythroid progenitors, could also be involved in the anemia observed in Parp-2−/− mice.
PARP-2-deficient mice show impaired differentiation of erythroid cells
To evaluate the impact of PARP-2-deficiency on erythroid progenitor development, we tested the ability of wild-type and Parp-2−/− cells from BM and spleen to form BFU-E and CFU-E in methyl cellulose. In these assays, Parp-2−/− cells from both BM and spleen displayed a significant decreased capacity to form BFU-E and CFU-E than wild-type cells, suggesting an early defect of PARP-2-deficient erythroid progenitors (Figure 3a).
To further characterize the role of PARP-2 in erythropoiesis at later stages, we also examined erythroid maturation in BM and spleen using the expression of the cell-surface markers Ter119 and CD71 and the forward scatter (FSC) parameter34 (Supplementary Figure S3A). We observed an increase in both the percentage and the absolute number of Ter119+ cells in BM and spleen of Parp-2−/− compared with wild-type mice (Figures 3b and c). The Ter119+ compartment showed a significant increase in the number of proerythroblasts (proE: Ter119medCD71high), basophilic erythroblasts (Ery.A: Ter119highCD71highFSChigh) and orthochromatic erythroblasts (Ery.C: Ter119highCD71lowFSClow) in both BM and spleen from Parp-2−/− compared with wild-type mice (Figure 3d). An increase in the number of late basophilic and polychromatophilic (Ery.B: Ter119highCD71highFSClow) was also observed, albeit statistically significant differences were not achieved in BM (Figure 3d). The imbalanced ratio of Ery.A/Ery.B, as an index of maturation, in BM and spleen from Parp-2−/− mice points toward a partial block in the transition from immature to mature RBC precursors (Figure 3e), suggesting that erythropoiesis in Parp-2−/− mice may not provide adequate reticulocytosis to compensate the massive hemolysis.
To determine possible causes behind the limited functionality of PARP-2-deficient erythroid progenitors, we analyzed their cell-cycle distribution. BM cells were isolated 1 h after 5-bromodeoxyuridine (BrdU) injection of mice, stained for surface expression of CD71 and Ter119 and the cell-cycle status were assessed by BrdU staining to detect DNA synthesis and with DAPI to gauge the amount of DNA per cell (Supplementary Figure S3B). Flow cytometry data revealed that Parp-2−/− erythroid progenitors exhibit a G2/M cell-cycle arrest (Figures 3f and g). In contrast, no alteration of either erythroid differentiation or the cell-cycle profile was observed in PARP-1-deficient erythroid progenitors (Supplementary Figure S4). Similar to the previously mentioned accumulation of micronuclei, the accumulation of cells in G2/M is reminiscent of RS.
RS limits the functionality of PARP-2-deficient erythroid progenitors
To directly evaluate the presence of RS in Parp-2−/− erythroblasts, we first used flow cytometry to monitor the phosphorylation of histone H2AX (γ-H2AX), a sensitive indicator of DNA injury.35 The percentage of γ-H2AX-positive cells was significantly higher in Parp-2−/− erythroblasts than that noted in wild-type cells (Figures 4a and b). Noteworthy, the increase on γ-H2AX was limited to S/G2 cells, a hallmark of RS36, 37 (Figure 4c). Next, we monitored on sorted Ter119+ erythroblasts the phosphorylation status of CHK1 on S345, a target of the ataxia-telangiectasia mutated (ATM) and Rad3 related (ATR) kinase that coordinates the response to RS.38 Our results revealed increased levels of phosphorylation of CHK1 in Parp-2−/− compared with wild-type cells (Figure 5). Of note, ATR has also been demonstrated to phosphorylate H2AX during replication fork blockage.39 We have also observed an increased level of replication protein A (RPA) phosphorylation on S4/S8 (Figure 5), triggered by ATM and DNA-PK (DNA-dependent protein kinase) kinases in response to RS.40
Gene expression analyses of PARP-2-deficient erythroblasts reveal the activation of p53-dependent DNA-damage response pathways
To gain further insights into the effect of PARP-2 deficiency on erythroblasts, we performed microarray on sorted Ter119+ BM erythroblasts from Parp-2−/− and wild-type mice. Clustering of all genes that changed between Parp-2−/− and wild-type erythroblasts is represented in Supplementary Figure S5. Gene set enrichment analysis (GSEA)41 showed a significant enrichment for p53-dependent signaling pathways in Parp-2−/− compared with wild-type erythroblasts (Figures 6a and b). For instances, p53-dependent cell-cycle regulator genes, such as p21 (Cdkn1a), Ccng1, Tp53inp1, as well as the cell-cycle associated gene, Ccnb1ip1, were markedly increased in Parp-2−/− erythroblasts (Figures 6b and c).
In addition, proapoptotic p53-dependent genes (Bax, Puma and Phlda3) were also overexpressed in Parp-2−/− cells (Figures 6b and c). Accordingly, higher levels of apoptosis, measured by immunostaining of active caspase-3 and immunofluorescence microscopy, were also observed in Parp-2−/− compared with wild-type erythroblasts (Figure 6d). These data prompted us to perform a genetic rescue experiment exploring the role of Puma in DNA-damage-mediated apoptosis of erythroblasts, caused by PARP-2 deficiency. This analysis suggested that loss of immature erythroblasts was due to p53-mediated apoptosis as, remarkably, Puma deficiency restores hematocrit in Parp-2−/− mice to wild-type levels (Figure 6e).
Impaired fetal liver erythropoiesis in Parp-2−/− embryos is amplified by loss of p21, resulting in lethality of newborn double-deficient mice
To investigate the functional interaction between PARP-2 and the cell-cycle regulator p21, we crossed Parp-2−/− with p21−/− mice and the resulting Parp-2+/−p21+/− mice were intercrossed in an attempt to generate PARP-2-deficient mice lacking p21. Interestingly, PARP-2/p21-double-null mice died at newborn stage (Figure 7a and Supplementary Table S1). At 14.5 days postcoitum (E14.5), PARP-2/p21-double-deficient embryos were pale (Figure 7b), suggesting exacerbated anemia. Noteworthy, PARP-1/p21-double-null adult mice were obtained at the normal Mendelian frequency (data not shown).
The fetal liver is the major tissue for definitive erythropoiesis during mid-gestation.1 Interestingly, PARP-2-null fetal liver displayed a significant decrease in cell number compared with wild-type or p21−/− fetal liver. This phenotype was strengthened by loss of p21 (Figure 7c). Further examination of erythrocyte development by flow cytometry revealed a significant decrease in both the percentage and the absolute number of Ter119+ cells in fetal liver from Parp-2−/− embryos that was also aggravated by the loss of p21 (Figures 7d and e). This result was confirmed by fetal liver immunostaining of Ter119 and microscopy analysis (Supplementary Figure S6). The Ter119+ compartment showed a significant decrease in the number of Ery.A and Ery. B cells in fetal liver from PARP-2/p21-double-null embryos compared with fetal liver from wild-type, p21−/− or Parp-2−/− embryos (Figures 7d–f). In addition, Parp-2−/− fetal liver exhibited a significant decrease in the number of Ery.B cells compared with wild-type or p21−/− fetal liver (Figures 7d–g).
Next, we assessed the cell-cycle status of the erythroblast compartments in fetal liver from embryos of the four genotypes. We detected a G2/M arrest in the Parp-2−/− fetal liver erythroblasts (Figures 7h and i), which was only slightly modified by loss of p21. However, p21 deficiency significantly altered G0/G1 cell-cycle checkpoint in Parp-2−/− Ery.B and Ery.C cells (Figures 7h and i). No differences in erythroblast development was observed between p21−/− and wild-type embryos (Figure 7).
Altogether, these data suggest a functional interaction between PARP-2 and p21 in the maintenance of fetal liver erythropoiesis, characterized by a rapid growth and little reserve capacity in the erythropoietic system,42 that impact on embryonic development.
PARP-2 deficiency compromises mouse survival in the context of enhanced hemolysis
Whereas the chronic anemia present on PARP-2-deficient mice did not overtly alter their lifespan, it could have more profound defects in the context of stress-driven compensatory erythropoiesis. To test this, we challenged wild-type and Parp-2−/− mice with phenylhydrazine (PHZ), a compound that induces oxidative denaturation of hemoglobin resulting in a rapid destruction of RBCs and severe hemolytic anemia.43 Mice were injected with PHZ on days 0, 1 and 3 and survival was monitored for 15 days. PARP-2 deficiency severely limited the survival in response to treatment, and eight out of nine Parp-2−/− mice died within 7 days after the first PHZ injection, whereas all but one control mouse survived the hemolytic challenge (Figure 8a). Accordingly, Parp-2−/− mice exhibited a larger drop in hematocrit compared with wild-type mice on day 5 after the first PHZ injection (Figure 8b). Despite a higher compensatory increase in EPO levels (Figure 8c), the reticulocyte index at day 5 after the first PHZ injection was significantly decreased in Parp-2−/− mice (Figure 8d), suggesting that defective erythropoiesis is responsible for the enhanced anemia-induced lethality of Parp-2−/− mice.
To further evaluate PHZ-induced erythropoiesis, maturation stages of erythroid cells from BM and spleen were analyzed at day 5 after the first PHZ injection. No differences were observed in BM and spleen cellularity between both genotypes (Figure 8e). Flow cytometric analysis revealed a significant decrease in the percentage and number of Ter119+ cells in BM from Parp-2−/− mice compared with wild-type mice, mainly due to a sharp decrease in Ery.B cell number (Figures 8f–h). However, a significant increase in the total number of Ter119+ cells was observed in the spleen of Parp-2−/− mice compared with wild-type mice, because of the accumulation of the more immature ProE and Ery.A erythroblasts (Figures 8f–h). Nevertheless, BM and spleen from Parp-2−/−-treated mice exhibit an imbalanced ratio of immature/mature erythroblasts established by calculating the ratio of Ery.A/Ery.B numbers (Figure 8i). Altogether, our data indicate that PARP-2 becomes essential for survival in conditions that demand an increased production of RBCs.
Discussion
Although anemia is the most common blood disorder,8 the molecular mechanisms governing erythrocyte number persist largely enigmatic. Here we have shown that deletion of PARP-2, but not PARP-1, in mice leads to chronic anemia providing a novel role for PARP-2 in erythropoiesis. Anemia arises even in the presence of increased EPO levels on PARP-2-deficient mice, which reflects a futile compensatory response that fails to provide sufficient erythropoiesis. Although chronic anemia observed in PARP-2-deficient mice is compatible with life at steady-state conditions, mice died rapidly when exposed to conditions that demand a higher production of RBCs. When we examined the erythroid maturation from the proerythroblast to late orthochromatic erythroblast stage, we found that the rate of maturation of BM Parp-2−/− erythroid cells at steady-state conditions was significantly reduced and was accompanied by an increase in the size of proerythroblasts and basophilic erythroblast populations. This might reflect a block in differentiation and subsequent apoptosis of precursor cells limiting the accumulation of damaged cells. Indeed, we observed an increased percentage of active caspase-3-positive erythroblasts in the absence of PARP-2. Moreover, the chronic anemia in Parp-2−/− mice was abolished in a Puma-deficient background, similarly to what we previously observed in response to γ-irradiation.26
Further evidence for a role of PARP-2 in erythroid development is provided by the presence of Howell–Jolly bodies in RBCs from Parp-2−/− mice. Howell–Jolly bodies are a marker of genotoxicity, which arise when replicating cells are subjected to chromosomal breakage or chromosomal loss by mitotic spindle dysfunction.44, 45 Our data are thus in agreement with previous in vitro studies that proposed a role for PARP-2 in chromosome segregation during cell division.21, 46 For instance, Menissier de Murcia et al.46 found a high proportion of aberrant anaphases in Parp-2−/− mouse embryonic fibroblasts following N-methyl-N-nitrosourea treatment, and a significant fraction of anaphase cells contained one or more lagging (nonsegregated) chromosomes. Of note, segregation problems led to chromatid breaks, which occurred more frequently in centromeric regions in Parp-2−/− cells, thus providing evidence for a possible role for PARP-2 in the maintenance of centromeric heterochromatin integrity,47 and a role in accurate chromosome segregation.21 Interestingly, it was shown that RS can lead to chromosome segregation problems that can be detected as anaphase bridges and micronuclei.31, 32
In agreement with a role of PARP-2 in maintaining genomic stability,9 our data indicate that PARP-2 deficiency in erythroblasts results in DNA damage, essentially occurring in replicating cells, as indicate by the accumulation of γ-H2AX in S phase. γ-H2AX formation has normally been associated with the induction of double strand breaks after exposure to DNA-damaging agents.48 However, a number of reports have shown phosphorylation of H2AX after RS,49, 50, 51, 52 which might be mediated in part by ATR.39 Indeed, the increased phosphorylation of CHK1 is indicative of an active RS-driven ATR response on PARP-2-deficient erythroid progenitors. This signaling cascade is associated with a transcriptional activation of p53-dependent cytostatic and apoptotic signals in Parp-2−/− erythroblasts, although we cannot rule out that altered gene expression in Parp-2−/− cells may be caused by a direct role of PARP-2 in transcription regulation.53 Moreover, lethality of PARP-2/p21-double-null pups, due to a defect in fetal liver erythropoiesis, suggests an accumulation of RS in the absence of PARP-2.54, 55 Altogether, these data suggest a critical role of PARP-2 in limiting RS in the highly divided erythroblast population. Remarkably, some of the described biological processes in which PARP-2, but not PARP-1, has been specifically involved are related with high proliferative cell rate such as thymopoiesis,19, 20 spermatogenesis21 and hematopoiesis under stress conditions.26 This bias for pathologies on tissues with high proliferative index has previously been noted on other mutant mice with a compromised response against RS, such as ATR-hypomorphic mice.56
Altogether, our results are consistent with a model (Supplementary Figure S7) whereby PARP-2 is specifically required to limit RS in erythroid progenitors. In the absence of PARP-2, highly divided erythroblasts accumulate RS leading to genomic instability that triggers the proximal ATR/H2AX/CHK1 DNA-damage response, which finally activates p53/p21 response resulting in G2/M arrest and apoptosis. Although damaged erythroblasts might arrive to circulation, they have a shorter lifespan. Taken together, deficient erythropoiesis and shortened RBC lifespan conduce to chronic anemia observed in Parp-2−/− mice. Whereas a compensatory increase in EPO production contributes to maintain erythropoiesis in Parp-2−/− mice, this response is insufficient in the context of enhanced hemolysis.
Although mounting evidence has accumulated indicating a role for PARP proteins at replication forks, most of these works were carried out with nonselective inhibitors, and pointed towards a role for PARP-157, 58, 59, 60, 61 and to less extent PARP-2 on this response.61 Moreover, all these works were carried out in vitro and to what extent this replication-linked role of PARP had in the context of a living organism remained unknown. Our results provided strong evidence that PARP-2, but not PARP-1, is required for efficient erythropoiesis in mice by limiting RS on erythroid progenitors, a function that becomes essential in the context of an enhanced demand for the production of RBCs.
Nowadays, there is considerable excitement about the prospect of anticancer compounds that act through targeting PARP proteins, although non-isoform-selective PARP inhibitors are available.14 Our observations reveal potential off-target effects that may arise from the use of such compounds.62 In addition, the specific role of PARP-2 in RS, a major driver of genomic instability,63, 64, 65 may have implications in the design and use of drugs targeting PARP proteins.
Materials and Methods
Animals and treatments
Parp-1−/−, Parp-2−/−, Puma−/− and p21−/− mice have been described previously.46, 66, 67, 68 P21−/− mice were bred with Parp-1−/− or Parp-2−/− mice, whereas Puma−/− mice were bred with Parp-2−/− mice, to generate heterozygous mice, which were then bred to generate all genotypes. Genotyping was performed by PCR analysis using tail DNA as described.46, 68, 69, 70 To induce acute anemia, mice were injected intraperitoneally with PHZ (Sigma-Aldrich, St. Louis, MO, USA) at 40 mg/kg on day 0, 1 and 3. All mice were in C57BL6 background and were kept under pathogen-free conditions in the animal house facility at the Barcelona Biomedical Research Park (Barcelona, Spain). Animal studies were approved by the Institutional Animal Care and Use Committee.
Blood analysis
For cell analysis, peripheral blood from 10- to 12-week-old mice was collected in EDTA-coated vials. Blood cell parameters were measured immediately after sample collection using an Abacus Junior Vet Hematology Blood Analyzer (Diatron, Hialeah, FL, USA). Blood smears were air dried and stained with May–Grünwald Giemsa for routine analysis. Differential cell counts were made blind on more than 1000 cells on random areas of slides.
EPO levels in plasma were determined by ELISA Assay Kit (R&D Systems, Minneapolis, MN, USA) in accordance with the manufacturer's instructions. Haptoglobin and hemoglobin levels in serum were determined by ELISA Assay Kits (Abcam, Cambridge, UK) in accordance with the manufacturer's instructions. Serum concentrations of total and direct bilirubin were determined by using a Bilirubin Assay Kit (BioAssay Systems, Hayward, CA, USA) in accordance with the manufacturer's instructions. Indirect bilirubin was determined by subtracting the concentration of direct bilirubin from the total bilirubin concentration. Iron levels in serum were measured on the Cobas 8000-701 Clinical Analyzer (Roche, Foster City, CA, USA).
Histological analysis
To study in situ splenic hemosiderin deposits, a Perl's iron stain technique was performed in 3 μm sections of samples from spleen of wild-type and Parp-2−/− mice. The staining was performed by using an Automated Stain System (Dako Artisan, Dako, Glostrup, Denmark), following the manufacturer's recommendations (Dako Artisan Iron Stain Kit, Dako Artisan). Hemosiderin was identified as blue deposits within cells. For fetal liver histological examination, embryos were fixed in formalin and embedded in paraffin for subsequent processing. Consecutive 2.5-μm sections were treated with citrate for antigen recovery and processed for immunohistochemistry with antibody against Ter119 (BD Biosciences, San Jose, CA, USA). Hematoxylin was used to counterstain. Immunohistochemistry slides were scanned with a MIRAX digitalized system (Zeiss, Oberkochen, Germany).
Flow cytometry and cell sorting
Cell suspensions were washed in PBS, resuspended in PBS containing 0.5% BSA and incubated with antibodies on ice for 30 min. Incubation with biotin-labeled antibodies was followed by incubation with streptavidin conjugates. Enucleated cells were lysed using ACK Lysis Buffer (BioWhitaker, Verviers, Belgium). Cell doublets were excluded from all analyses and dead cells were excluded by the use of DAPI. For intracellular staining, cells were stained for cell-surface markers, fixed and made permeable by using an intracellular staining buffer set (BD Biosciences), and then stained with specific antibody or isotype control. The following antibodies were used: PE-conjugated Ter119 (Ter119; BD Biosciences), APC-conjugated CD71 (R17217; eBiosciences, San Diego, CA, USA), phospho-H2AX (S139) (JPW301; Millipore, Billerica, MA, USA), phospho-CHK1 (S345) (133D3; Cell Signaling Technology, Danvers, MA, USA), phospho-RPA32 (S4/8) (Bethyl Laboratories, Montgomery, TX, USA), PerCP-conjugated streptavidin (BioLegend, San Diego, CA, USA) and goat Alexa Fluor 488- or 555-conjugated (Invitrogen, Carlsbad, CA, USA) anti-rabbit and anti-mouse antibody. Samples were acquired with an LSRII cytometer (BD Biosciences) and the data were analyzed with the DIVA (BD Biosciences) and FlowJo (TreeStar) software (FlowJo, Ashland, OR, USA). Cell sorting was performed in a FACS Aria II SORP (BD Biosciences).
Reticulocyte index
Reticulocytes in blood were determined by staining with thiazole orange and analyses by flow cytometry. The reticulocyte index was calculated as reticulocyte (%)x(hematocrit (%)/normal mouse hematocrit (%)).
RBC turnover assay
RBCs were labeled in vivo with biotin by intravenous injection of mice with 60 mg/kg of NHS-LC-biotin (N-succinimidyl-6-(biotinamido)hexanoate) (Pierce Biotechnology, Rockford, IL, USA). The percentage of biotinylated cells in circulating blood was determined at regular intervals. For monitoring, blood samples were obtained from the tail vein, diluted in PBS, labeled with APC-conjugated streptavidin (BD Biosciences) and FITC-conjugated Ter119 and analzsed by flow cytometry.
Colony formation assays
Cell suspensions were mixed with MethoCult M3434 methyl cellulose semisolid medium (StemCell Technologies, Grenoble, France), plated in 35-mm dishes and cultured at 37 °C with 5% CO2. CFU-E colonies were scored on day 3, whereas BFU-E colonies were scored on day 7.
Cell cycle analysis
For in vivo BrdU labeling experiments, 10- to 12-week-old mice received a single intraperitoneal injection of BrdU (BD Biosciences; 1 mg/6 g of mouse weight). Cells were isolated from mice 1 h after injection, surface stained for CD71 and Ter119, fixed, permeabilized and intracellularly stained with anti-BrdU-FITC using the BrdU Flow Kit (BD Biosciences) and DAPI to gauge the amount of DNA per cell.
Immunofluorescence microscopy
Nucleated Ter119+ cells from BM were sorted on slides, fixed in 2% paraformaldehyde/PBS (w/v) for 10 min at room temperature, washed in PBS and permeabilized in a 0.1% sodium citrate/0.1% Triton X-100 solution. Slides were then incubated in blocking solution, and incubated overnight with a rabbit polyclonal anti-active caspase-3 (R&D Systems) primary antibody at 4 ºC. After washing, sections were incubated for 1 h at room temperature with goat anti-rabbit-conjugated Alexa Fluor 488, counterstained with DAPI, mounted and examined with an inverted fluorescence microscopy (Olympus BX61 microscope, Tokyo, Japan).
Western blot
Nucleated Ter119+ sorted cells from wild-type and Parp-2−/− mice were washed once with PBS, and total protein extracts were prepared by directly adding 2 × NuPAGE LDS sample buffer and then incubated for 5 min at 95 °C. Samples were resolved by SDS-PAGE and analyzed by standard western blotting techniques. Antibodies against CHK1 (2G1D5; Cell Signaling Technology), phospho-CHK1 (S345), β-actin (Sigma-Aldrich) and phospho-RPA32 (S4/8) were used. Signals were developed using an Enhanced Chemiluminiscence Kit (Amersham-Pharmacia Biotech, Buckinghamshire, UK), according to the manufacturer’s instructions.
RNA extraction, reverse transcription and PCR
Total RNA was isolated from sorted nucleated Ter119+ cells from BM of wild-type and Parp-2−/− mice by using the RNeasy Total RNA Isolation Kit (Qiagen, Valencia, CA, USA) with on-column DNase I (Qiagen) digestion, following the manufacturer's instructions. Three independent experiments with different batches of cells were carried out. Quantity and integrity of the RNA was assessed by nanoelectrophoresis using the Pico Lab-on-a-Chip assay for total eukaryotic RNA using Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA). Total RNA (5 ng) from each sample was used for cDNA synthesis using the Ovation Pico WTA System V2 (NuGEN Technologies, San Carlos, CA, USA). Specific primers for the different genes are included in Supplementary Table S2. qPCR was carried out using 15 ng cDNA per sample using platinum SYBR Green Master mix (Applied Biosystems, Warrington, UK). Assays were run in triplicate on the ABI 7900HT system (Applied Biosystems). Expression levels of β-actin were used to normalize the amount of the examined transcript.
Gene expression array
Total RNA was isolated from sorted nucleated Ter119+ cells derived from pooled BM of three mice from each genotype. Microarray expression analysis was performed from samples of two independent experiments. Quantity and integrity of the RNA was assessed by nanoelectrophoresis using the Pico Lab-on-a-Chip assay for total eukaryotic RNA using Bioanalyzer 2100 (Agilent Technologies). Only samples with high integrity (RNA integrity number >8) were subsequently used in microarray experiments. Microarray expression profiles were obtained using the Affymetrix GeneChip Mouse Gene 1.0 ST Array (Affymetrix, Santa Clara, CA, USA) and the GCS3000 Affymetrix Platform (Affymetrix). Briefly, 10 ng of total RNA from each sample was amplified using the Ovation Pico WTA System (NuGEN Technologies) and sense transcript cDNA (ST-cDNA) was generated using the WT-Ovation Exon Module (NuGEN Technologies). After, ST-cDNA was fragmented and labeled with the FL-Ovation cDNA Biotin Module V2 (NuGEN Technologies), and the biotinylated cDNA was hybridized to Affymetrix GeneChip Mouse Gene 1.0 ST Arrays. Following hybridization, the array was washed and stained, and finally scanned to generate CEL files for each array. Microarray data have been deposited into the Gene Expression Omnibus (GSE54864).
Statistical analysis
The log-rank test was used to determine the statistical of animal survival. All other statistical analyses used a t-test (two-tailed, assuming unequal variance). P-values of <0.05 were considered to indicate statistical significance. Bioinformatics statistical analysis of microarray data is indicated in the Supplementary Data.
Abbreviations
- PARP:
-
poly(ADP-ribose)polymerase
- EPO:
-
erythropoietin
- RPA:
-
replication protein A
- RBCs:
-
red blood cells
- BFU-E:
-
erythroid burst-forming unit
- CFU-E:
-
erythroid colony-forming unit
- β−NAD+:
-
β-nicotinamide adenine dinucleotide
- AID:
-
activation-induced cytidine deaminase
- RS:
-
replicative stress
- MCH:
-
mean corpuscular hemoglobin
- MCHC:
-
mean concentration of corpuscular hemoglobin
- MCV:
-
mean corpuscular volume
- BM:
-
bone marrow
- BrdU:
-
5-bromodeoxyuridine
- ATR:
-
ataxia-telangiectasia mutated and Rad3 related
- ATM:
-
ataxia-telangiectasia mutated
- GSEA:
-
gene set enrichment analysis
- PHZ:
-
phenylhydrazine
References
Hattangadi SM, Wong P, Zhang L, Flygare J, Lodish HF . From stem cell to red cell: regulation of erythropoiesis at multiple levels by multiple proteins, RNAs, and chromatin modifications. Blood 2011; 118: 6258–6268.
Palis J, Segel GB . Developmental biology of erythropoiesis. Blood Rev 1998; 12: 106–114.
Socolovsky M, Nam H, Fleming MD, Haase VH, Brugnara C, Lodish HF . Ineffective erythropoiesis in Stat5a(−/−)5b(−/−) mice due to decreased survival of early erythroblasts. Blood 2001; 98: 3261–3273.
Shearstone JR, Pop R, Bock C, Boyle P, Meissner A, Socolovsky M . Global DNA demethylation during mouse erythropoiesis in vivo. Science 2011; 334: 799–802.
Sankaran VG, Ludwig LS, Sicinska E, Xu J, Bauer DE, Eng JC et al. Cyclin D3 coordinates the cell cycle during differentiation to regulate erythrocyte size and number. Genes Dev 2012; 26: 2075–2087.
Wang F, Zhu Y, Guo L, Dong L, Liu H, Yin H et al. A regulatory circuit comprising GATA1/2 switch and microRNA-27a/24 promotes erythropoiesis. Nucleic Acids Res 2014; 42: 442–457.
Grover A, Mancini E, Moore S, Mead AJ, Atkinson D, Rasmussen KD et al. Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate. J Exp Med 2014; 211: 181–188.
Balarajan Y, Ramakrishnan U, Ozaltin E, Shankar AH, Subramanian SV . Anaemia in low-income and middle-income countries. Lancet 2011; 378: 2123–2135.
Yelamos J, Farres J, Llacuna L, Ampurdanes C, Martin-Caballero J . PARP-1 and PARP-2: new players in tumour development. Am J Cancer Res 2011; 1: 328–346.
Jungmichel S, Rosenthal F, Altmeyer M, Lukas J, Hottiger MO, Nielsen ML . Proteome-wide identification of poly(ADP-Ribosyl)ation targets in different genotoxic stress responses. Mol Cell 2013; 52: 272–285.
Rulten SL, Fisher AE, Robert I, Zuma MC, Rouleau M, Ju L et al. PARP-3 and APLF function together to accelerate nonhomologous end-joining. Mol Cell 2011; 41: 33–45.
Boehler C, Gauthier LR, Mortusewicz O, Biard DS, Saliou JM, Bresson A et al. Poly(ADP-ribose) polymerase 3 (PARP3), a newcomer in cellular response to DNA damage and mitotic progression. Proc Natl Acad Sci USA 2011; 108: 2783–2788.
Lord CJ, Ashworth A . The DNA damage response and cancer therapy. Nature 2012; 481: 287–294.
Wahlberg E, Karlberg T, Kouznetsova E, Markova N, Macchiarulo A, Thorsell AG, Pol E, Frostell A, Ekblad T, Oncu D, Kull B, Robertson GM, Pellicciari R, Schuler H, Weigelt J . Family-wide chemical profiling and structural analysis of PARP and tankyrase inhibitors. Nat. Biotechnol. 2012; 30: 283–288.
Leger K, Bar D, Savic N, Santoro R, Hottiger MO . ARTD2 activity is stimulated by RNA. Nucleic Acids Res. 2014; 42: 5072–5082.
Isabelle M, Moreel X, Gagne JP, Rouleau M, Ethier C, Gagne P et al. Investigation of PARP-1, PARP-2, and PARG interactomes by affinity-purification mass spectrometry. Proteome Sci 2010; 8: 22.
Oliver AW, Ame JC, Roe SM, Good V, de Murcia G, Pearl LH . Crystal structure of the catalytic fragment of murine poly(ADP-ribose) polymerase-2. Nucleic Acids Res 2004; 32: 456–464.
Troiani S, Lupi R, Perego R, Depaolini SR, Thieffine S, Bosotti R et al. Identification of candidate substrates for poly(ADP-ribose) polymerase-2 (PARP2) in the absence of DNA damage using high-density protein microarrays. FEBS J 2011; 278: 3676–3687.
Yelamos J, Monreal Y, Saenz L, Aguado E, Schreiber V, Mota R et al. PARP-2 deficiency affects the survival of CD4+CD8+ double-positive thymocytes. EMBO J 2006; 25: 4350–4360.
Nicolas L, Martinez C, Baro C, Rodriguez M, Baroja-Mazo A, Sole F et al. Loss of poly(ADP-ribose) polymerase-2 leads to rapid development of spontaneous T-cell lymphomas in p53-deficient mice. Oncogene 2010; 29: 2877–2883.
Dantzer F, Mark M, Quenet D, Scherthan H, Huber A, Liebe B et al. Poly(ADP-ribose) polymerase-2 contributes to the fidelity of male meiosis I and spermiogenesis. Proc Natl Acad Sci USA 2006; 103: 14854–14859.
Robert I, Dantzer F, Reina-San-Martin B . Parp1 facilitates alternative NHEJ, whereas Parp2 suppresses IgH/c-myc translocations during immunoglobulin class switch recombination. J Exp Med 2009; 206: 1047–1056.
Bai P, Houten SM, Huber A, Schreiber V, Watanabe M, Kiss B et al. Poly(ADP-ribose) polymerase-2 [corrected] controls adipocyte differentiation and adipose tissue function through the regulation of the activity of the retinoid X receptor/peroxisome proliferator-activated receptor-gamma [corrected] heterodimer. J Biol Chem 2007; 282: 37738–37746.
Bai P, Canto C, Brunyanszki A, Huber A, Szanto M, Cen Y et al. PARP-2 regulates SIRT1 expression and whole-body energy expenditure. Cell Metab 2011; 13: 450–460.
Szanto M, Brunyanszki A, Marton J, Vamosi G, Nagy L, Fodor T et al. Deletion of PARP-2 induces hepatic cholesterol accumulation and decrease in HDL levels. Biochim Biophys Acta 2014; 1842: 594–602.
Farres J, Martin-Caballero J, Martinez C, Lozano JJ, Llacuna L, Ampurdanes C et al. Parp-2 is required to maintain hematopoiesis following sublethal gamma-irradiation in mice. Blood 2013; 122: 44–54.
Paulson RF, Shi L, Wu DC . Stress erythropoiesis: new signals and new stress progenitor cells. Curr Opin Hematol 2011; 18: 139–145.
Van Putten LM . The life span of red cells in the rat and the mouse as determined by labeling with DFP32 in vivo. Blood 1958; 13: 789–794.
Connor J, Pak CC, Schroit AJ . Exposure of phosphatidylserine in the outer leaflet of human red blood cells. Relationship to cell density, cell age, and clearance by mononuclear cells. J Biol Chem 1994; 269: 2399–2404.
de JK, Emerson RK, Butler J, Bastacky J, Mohandas N, Kuypers FA . Short survival of phosphatidylserine-exposing red blood cells in murine sickle cell anemia. Blood 2001; 98: 1577–1584.
Chan KL, Palmai-Pallag T, Ying S, Hickson ID . Replication stress induces sister-chromatid bridging at fragile site loci in mitosis. Nat Cell Biol 2009; 11: 753–760.
Naim V, Rosselli F . The FANC pathway and mitosis: a replication legacy. Cell Cycle 2009; 8: 2907–2911.
Crasta K, Ganem NJ, Dagher R, Lantermann AB, Ivanova EV, Pan Y et al. DNA breaks and chromosome pulverization from errors in mitosis. Nature 2012; 482: 53–58.
Liu Y, Pop R, Sadegh C, Brugnara C, Haase VH, Socolovsky M . Suppression of Fas-FasL coexpression by erythropoietin mediates erythroblast expansion during the erythropoietic stress response in vivo. Blood 2006; 108: 123–133.
Fernandez-Capetillo O, Lee A, Nussenzweig M, Nussenzweig A . H2AX: the histone guardian of the genome. DNA Repair (Amst) 2004; 3: 959–967.
Toledo LI, Murga M, Zur R, Soria R, Rodriguez A, Martinez S et al. A cell-based screen identifies ATR inhibitors with synthetic lethal properties for cancer-associated mutations. Nat Struct Mol Biol 2011; 18: 721–727.
Syljuasen RG, Sorensen CS, Hansen LT, Fugger K, Lundin C, Johansson F et al. Inhibition of human Chk1 causes increased initiation of DNA replication, phosphorylation of ATR targets, and DNA breakage. Mol Cell Biol 2011; 25: 3553–3562.
Lopez-Contreras AJ, Fernandez-Capetillo O . The ATR barrier to replication-born DNA damage. DNA Repair (Amst) 2010; 9: 1249–1255.
Ward IM, Minn K, Chen J . UV-induced ataxia-telangiectasia-mutated and Rad3-related (ATR) activation requires replication stress. J Biol Chem 2004; 279: 9677–9680.
Liu S, Opiyo SO, Manthey K, Glanzer JG, Ashley AK, Amerin C et al. Distinct roles for DNA-PK, ATM and ATR in RPA phosphorylation and checkpoint activation in response to replication stress. Nucleic Acids Res 2012; 40: 10780–10794.
Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA 2005; 102: 15545–15550.
Socolovsky M, Fallon AE, Wang S, Brugnara C, Lodish HF . Fetal anemia and apoptosis of red cell progenitors in Stat5a−/−5b−/− mice: a direct role for Stat5 in Bcl-X(L) induction. Cell 1999; 98: 181–191.
Itano HA, Hirota K, Hosokawa K . Mechanism of induction of haemolytic anaemia by phenylhydrazine. Nature 1975; 256: 665–667.
Schlegel R, MacGregor JT, Everson RB . Assessment of cytogenetic damage by quantitation of micronuclei in human peripheral blood erythrocytes. Cancer Res 1986; 46: 3717–3721.
Heddle JA, Hite M, Kirkhart B, Mavournin K, MacGregor JT, Newell GW et al. The induction of micronuclei as a measure of genotoxicity. A report of the US Environmental Protection Agency Gene-Tox Program. Mutat Res 1983; 123: 61–118.
Menissier de Murcia J, Ricoul M, Tartier L, Niedergang C, Huber A, Dantzer F et al. Functional interaction between PARP-1 and PARP-2 in chromosome stability and embryonic development in mouse. EMBO J 2003; 22: 2255–2263.
Saxena A, Wong LH, Kalitsis P, Earle E, Shaffer LG, Choo KH . Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc. Hum Mol Genet 2002; 11: 2319–2329.
Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM . DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998; 273: 5858–5868.
Ward IM, Chen J . Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress. J Biol Chem 2001; 276: 47759–47762.
Furuta T, Takemura H, Liao ZY, Aune GJ, Redon C, Sedelnikova OA et al. Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes. J Biol Chem 2003; 278: 20303–20312.
Ewald B, Sampath D, Plunkett W . H2AX phosphorylation marks gemcitabine-induced stalled replication forks and their collapse upon S-phase checkpoint abrogation. Mol Cancer Ther 2007; 6: 1239–1248.
Gagou ME, Zuazua-Villar P, Meuth M . Enhanced H2AX phosphorylation, DNA replication fork arrest, and cell death in the absence of Chk1. Mol Biol Cell 2010; 21: 739–752.
Szanto M, Brunyanszki A, Kiss B, Nagy L, Gergely P, Virag L et al. Poly(ADP-ribose) polymerase-2: emerging transcriptional roles of a DNA-repair protein. Cell Mol Life Sci 2012; 69: 4079–4092.
Porlan E, Morante-Redolat JM, Marques-Torrejon MA, Andreu-Agullo C, Carneiro C, Gomez-Ibarlucea E et al. Transcriptional repression of Bmp2 by p21(Waf1/Cip1) links quiescence to neural stem cell maintenance. Nat Neurosci 2013; 16: 1567–1575.
Viale A, De FF, Orleth A, Cambiaghi V, Giuliani V, Bossi D et al. Cell-cycle restriction limits DNA damage and maintains self-renewal of leukaemia stem cells. Nature 2009; 457: 51–56.
Murga M, Bunting S, Montana MF, Soria R, Mulero F, Canamero M et al. A mouse model of ATR-Seckel shows embryonic replicative stress and accelerated aging. Nat Genet 2009; 41: 891–898.
Sugimura K, Takebayashi S, Taguchi H, Takeda S, Okumura K . PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA. J Cell Biol 2008; 183: 1203–1212.
Ray CA, Hashimoto Y, Herrador R, Neelsen KJ, Fachinetti D, Bermejo R et al. Topoisomerase I poisoning results in PARP-mediated replication fork reversal. Nat Struct Mol Biol 2012; 19: 417–423.
McLellan JL, O'Neil NJ, Barrett I, Ferree E, van Pel DM, Ushey K et al. Synthetic lethality of cohesins with PARPs and replication fork mediators. PLoS Genet 2012; 8: e1002574.
Min W, Bruhn C, Grigaravicius P, Zhou ZW, Li F, Kruger A et al. Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation. Nat Commun 2013; 4: 2993.
Bryant HE, Petermann E, Schultz N, Jemth AS, Loseva O, Issaeva N et al. PARP is activated at stalled forks to mediate Mre11-dependent replication restart and recombination. EMBO J 2009; 28: 2601–2615.
Fong PC, Boss DS, Yap TA, Tutt A, Wu P, Mergui-Roelvink M et al. Inhibition of poly(ADP-ribose) polymerase in tumors from BRCA mutation carriers. N Engl J Med 2009; 361: 123–134.
Burrell RA, McClelland SE, Endesfelder D, Groth P, Weller MC, Shaikh N et al. Replication stress links structural and numerical cancer chromosomal instability. Nature 2013; 494: 492–496.
Hu L, Filippakis H, Huang H, Yen TJ, Gjoerup OV . Replication stress and mitotic dysfunction in cells expressing simian virus 40 large T antigen. J Virol 2013; 87: 13179–13192.
Ichijima Y, Yoshioka K, Yoshioka Y, Shinohe K, Fujimori H, Unno J et al. DNA lesions induced by replication stress trigger mitotic aberration and tetraploidy development. PLoS One 2010; 5: e8821.
de Murcia JM, Niedergang C, Trucco C, Ricoul M, Dutrillaux B, Mark M et al. Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells. Proc Natl Acad Sci USA 1997; 94: 7303–7307.
Brugarolas J, Chandrasekaran C, Gordon JI, Beach D, Jacks T, Hannon GJ et al. Radiation-induced cell cycle arrest compromised by p21 deficiency. Nature 1995; 377: 552–557.
Villunger A, Michalak EM, Coultas L, Mullauer F, Bock G, Ausserlechner MJ . p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa. Science 2003; 302: 1036–1038.
Corral J, Yelamos J, Hernandez-Espinosa D, Monreal Y, Mota R, Arcas I et al. Role of lipopolysaccharide and cecal ligation and puncture on blood coagulation and inflammation in sensitive and resistant mice models. Am J Pathol 2005; 166: 1089–1098.
Garcia-Fernandez RA, Garcia-Palencia P, Sanchez MA, Gil-Gomez G, Sanchez B, Rollan E et al. Combined loss of p21(waf1/cip1) and p27(kip1) enhances tumorigenesis in mice. Lab Invest 2011; 91: 1634–1642.
Acknowledgements
We thank J Moreno, M Juan and S Capdevila for assistance with mice, R Gimeno, A Bigas and C Gekas for useful comments, O Fornes for cell sorting and flow cytometry assistance, E Puigdecanet and L Nonell for microarrays support and S Mojal for statistical analysis support, as well as A Strasser for Puma-deficient mice. This work was supported by the Spanish Ministerio de Economía y Competitividad (SAF2011-26900), Generalitat de Catalunya (2014/SGR/184) and Fundació La Marató de TV3 (20134130) to JY. Work in FD and VS is supported by the Centre National de la Recherche Scientifique, Université de Strasbourg, Ligue contre le Cancer (Equipe labellisée) and Laboratory of Excellence (LabEx) Medalis ANR-10-LABX-0034 'Programme d'investissement d'avenir'. AV and EO are supported by the Austrian Science Fund (FWF; W1101, MCBO). Work in OF-C laboratory is supported by the Spanish Ministry of Science (SAF2011-23753), Association for International Cancer Research (12-0229), Fundació La Marató de TV3 (20134130) and European Research Council (ERC-210520). AJL-C is a recipient of a postdoctoral fellowship from the Spanish Association Against Cancer (AECC). LL was a recipient of a postdoctoral fellowship from the Juan de la Cierva Program.
Author Contributions
JF and LL carried out most of the experiments presented in the paper. JM-C carried out RBC lifespan and blood cell analysis experiments. CM and AJL-C carried out pathology and microscopy experiments. JL performed gene expression array analysis. CA and EO provided technical assistance. JN and LF carried out blood cell analysis. FD, VS, AV and OF-C designed experiments, provided reagents and edited paper. JY planned and designed experiments, performed experiments and wrote the paper. All authors discussed the results and commented on the manuscript.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no conflict of interest.
Additional information
Edited by RA Knight
Supplementary Information accompanies this paper on Cell Death and Differentiation website
Rights and permissions
About this article
Cite this article
Farrés, J., Llacuna, L., Martin-Caballero, J. et al. PARP-2 sustains erythropoiesis in mice by limiting replicative stress in erythroid progenitors. Cell Death Differ 22, 1144–1157 (2015). https://doi.org/10.1038/cdd.2014.202
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/cdd.2014.202
This article is cited by
-
PARP2 promotes Break Induced Replication-mediated telomere fragility in response to replication stress
Nature Communications (2024)
-
Hematological Toxicity of PARP Inhibitors in Metastatic Prostate Cancer Patients with Mutations of BRCA or HRR Genes: A Systematic Review and Safety Meta-analysis
Targeted Oncology (2024)
-
Synergistic immunotherapy targeting cancer-associated anemia: prospects of a combination strategy
Cell Communication and Signaling (2023)
-
PARP2 poly(ADP-ribosyl)ates nuclear factor erythroid 2-related factor 2 (NRF2) affecting NRF2 subcellular localization
Scientific Reports (2023)
-
A new wave of innovations within the DNA damage response
Signal Transduction and Targeted Therapy (2023)