Figure 5 | Cell Death & Differentiation

Figure 5

From: Proteolysis of Ambra1 during apoptosis has a role in the inhibition of the autophagic pro-survival response

Figure 5

Ambra1 is a caspase and calpain substrate. Radioactively labeled recombinant Ambra1-WT (a) or Ambra1-D482A mutant (b) was subjected to an in vitro caspase-cleavage assay by using recombinant active caspase-3, -6, -7 or -8. Samples were subjected to SDS-PAGE and protein fragments visualized by autoradiography. (c) Caspase-3 cleavage of radioactively labelled recombinant Ambra1-WT and Ambra1-D482A mutant. (* points to Ambra1-D482A mutant protein fragment with apparent slightly different molecular weights compared with wild-type protein, likely due to alternative cleavage). (d) Radioactively labeled recombinant Ambra1 was subjected to an in vitro calpain-cleavage assay by using recombinant active calpain-I and -II. Samples were subjected to SDS-PAGE and protein fragments visualized by autoradiography (N=3). (e) Radioactively labeled recombinant Ambra1-WT or Ambra1-D482A mutant were subjected to an in vitro calpain-cleavage assay by using recombinant active calpain-I. Samples were subjected to SDS-PAGE and protein fragments visualized by autoradiography (N=3)

Back to article page