Figure 4 | Cell Death & Differentiation

Figure 4

From: Proteolysis of Ambra1 during apoptosis has a role in the inhibition of the autophagic pro-survival response

Figure 4

Enforced expression of Ambra1 inhibits apoptosis. (a–c) 2F cells were infected with a retroviral vector encoding Flag-Ambra1 or β-Gal. Protein extracts were analyzed by western blot using anti-Flag (Ambra1) or anti-Gapdh antibodies (a, N=3). Cells were incubated with staurosporine (2 μM, 6 h) or etoposide (5 μg/ml, 24 h), cell viability was evaluated by MTT assay (b) and cell death was measured by FACS analysis (c). Data represent average and S.D.s of three independent experiments (P<0.05)

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