Figure 4 | Cell Death & Differentiation

Figure 4

From: Mitochondrial dysfunction triggered by loss of HtrA2 results in the activation of a brain-specific transcriptional stress response

Figure 4

Activation of the ISR in brains from HtrA2 KO mice and IPD patients. (a) ATF3 and HERP are upregulated in HtrA2 KO brain tissue, but not Hsp60 or ClpP. Basal ganglia were dissected from brains of WT and HtrA2 KO mice at P27. Transcript levels are shown relative to WT. Mean values±S.E.M.; n=3–5 in each group. (b) ATF3 and HERP expression are specifically upregulated in HtrA2 KO brain tissue. Brain, heart, kidney, liver and skeletal muscle tissue were isolated from WT and HtrA2 KO mice at P22. Transcript levels of ATF3 and HERP are shown relative to WT. Mean values±S.D.; n=3 in each group. Statistically significant values (one-way ANOVA with Bonferroni's post-test) relative to WT are indicated. (c) CHOP and HERP are significantly upregulated in a subset of IPD patients. Transcript levels are shown relative to one control patient. Means are indicated by the horizontal bars. Purple dots correspond to a selected set of IPD patients displaying levels of the CHOP transcript above the mean value. P-values (two-tailed t-test assuming unequal variance) relative to control are indicated. *** for P<0.001, ** for P<0.01 and * for P<0.05

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