Figure 1

From: Insulin exerts direct, IGF-1 independent actions in growth plate chondrocytes

Figure 1

Insulin promotes growth of fetal metatarsal bones in vitro. (a) Metatarsalbones from E17.5 wild-type mouse embryos were cultured for 6 days in the absence or presence of insulin or IGF-1. (b) Quantitation of the actual length of metatarsal bones from (a) as shown. *P<0.05, n=6. (c) Representative histological images of metatarsal bones treated with insulin or IGF-1, with none treatment as control. PZ, proliferating zone; HZ, hypertrophic zone; MZ, mineralizing zone. (d) Quantitation of the proliferating zone of the cartilage rudiments from (c). *P<0.05, n=6. (e) Representative histological sections of metatarsal bones treated with insulin or IGF-1 after staining with antibody against PCNA as described in the section on ‘Materials and methods’. Sections were counterstained with hematoxylin. Scale bar=50 µm. (f) Quantitation for the percentage of PCNA+ cells from (e). *P<0.05, n=6. IGF-1 treatment shows more dramatic effects on the total length in the cultures as positive control. CON, none treatmentcontrol; IGF-1, insulin-like growth factor-1; INS, insulin.