Osteoporosis is a major health problem because of the associated low-trauma fractures, morbidity and mortality associated with it, and the cost of treatment. While statins are not used, nor even considered as a potential therapeutic class for osteoporosis, some studies, dating back to 1999, have shown a potential beneficial effect of statins on bone metabolism (1). Plausible molecular mechanisms include the inhibition of the mevalonate pathway, the same pathway that is inhibited by bisphosphonates (2), activation of the bone morphogenetic protein-2 promoter (BMP-2), which acts as an osteoblast growth factor (3,4) and reduction of oxidative stress through up-regulation of NAPDH oxidase 4 (Nox4) (5). Although some studies have shown that statins reduce fracture risk, the issue is far from being settled (613). The largest meta-analysis, which included 151 500 subjects of whom 9 946 were statin users, suggested that statin use was associated with reduced fracture risk, but other analyses have not been confirmatory (8).

Hypotheses linking lipids to skeletal health include several potential mechanisms. Age-related increases in interleukin-6 (IL-6) and C-reactive protein (CRP) (14) have been associated with osteoporosis (15), especially in overweight adults (16). Oxidized lipids, characteristic of hyperlipidemia, modulate the resorptive effects of parathyroid hormone (PTH) (17), inducing the expression of RANKL (18) and suppressing the differentiation of bone marrow stromal cells to osteoblasts (19). In this study, we sought to gain additional information beyond what has been reported so far (2022) on bone mineral density among postmenopausal women with osteoporosis based upon their history of statin use and fragility fractures. We conducted this test of the hypothesis because new preclinical evidence has been published recently, while clinical studies, especially on a population such as ours, are lacking.

Materials and methods

The study included 610 consecutive patients who were treated for osteoporosis at the “C. I. Parhon”, National Institute for Endocrinology, Romania in the National Program for Osteoporosis which offered free medication. The inclusion criteria was a diagnosis of osteoporosis as defined by densitometric parameters with no exclusion criteria regarding these parameters or lipids.

The data on age and age at menopause, in years, were obtained from the patients' records or from the patient directly. The presence or absence of a fracture was determined for each patient by spine radiography as recorded in the patient's file only by the primary radiologist who knew the purpose of the evaluation which was to determine whether or note vertebral fractures were present. Lumbar spine BMD (g·cm−2) was measured by dual energy X-ray absorptiometry (DXA-GE-Lunar; L1-L4 site). Total cholesterol, HDL-cholesterol and triglycerides were measured by spectrophotometric enzymatic colorimetric tests using a COBAS INTEGRA 400 plus (Roche) and LDL-cholesterol by using the formula LDLc=Total cholesterol-(Triglycerides/5 + HDLc). Normal ranges were considered to be: 120–200 mg·dL−1 for total cholesterol, 35–150 mg·dL−1 for triglycerides, 40–65 mg·dL−1 for HDLc, 60–160 mg·dL−1 for LDLc. Body mass index (BMI) was calculated by the formula BMI=weight·height−2, where height is expressed in meters and weight in kilograms. We considered patients with a T-score between −1.0 and −2.49 to be osteopenic and those with a T-score<−2.5 to have osteoporosis (23). This study included patients seen between January 2000 and March 2011.

The statistical analysis was carried out using EpiInfo 7 provided by the Center for Disease Control and Prevention (CDC, USA). Using this program we measured means, standard deviations, multivariate linear regressions and single linear regressions expressed as r2 or r and type 2, two tailed, P-values expressed as P. For the multivariate regressions, BMD was the dependent variable while BMI, LDLc, HDLc, TG and total Cholesterol were independent variables. A P value of <0.05 was considered to be statistically significant. The β value was 0.20 (a B:α ratio of 4:1). All study participants gave written or oral consent.


All subjects were postmenopausal women (mean years since menopause: 15.8±8.9 years) with osteoporosis (mean T score: −3.16±0.81) and were grouped according to age at diagnosis (<50 years, 51–60 years, 61–70 years and >70 years) and the presence or absence of a history of fragility fracture (Table 1). All patients received vitamin D and calcium supplements. 402 patients received bisphosphonates, 151 selective estrogenreceptor modulators (SERMs) and 46 received vitamin D and calcium alone. 150 patients also received statins, 145 patients received both bisphosphonates and statins. Statin usage could not be established in 94 patients. There were no statistically significant differences found between vitamin D levels and calcium levels for these patients (P>0.05 for both variables in the entire cohort and all the subgroups).

Table 1 Baseline patient characteristics

The included parameters as well as the ones that were not included in the correlation were based on the fact that we designed this study to reflect, as much as possible, a real world scenario. The analysis was based on the known correlation between osteoporosis and age, and between lipid levels with age. We chose to select subgroups by decades of age because this allowed us to more easily show a clinically significant correlation than by using logistic regression which would not have pinpointed specific subgroups as clearly.

BMD and the Lipid Profile

The multivariate regression for the entire study population showed no correlation between BMD (dependent variable) and BMI, cholesterol, LDLc, HDLc and triglycerides (independent variables) (n=613, r2=0.02, P>0.05). Even after grouping the patients according to fractures, no correlation was seen in the subjects with (n=380, r2=0.03, P>0.05) or without previous fracture (n=232, r2=0.06, P>0.05).

We next examined subgroups according to age. In subjects whose diagnosis of osteoporosis was made between ages 51 and 60, a significant multivariate correlation was seen between BMD and the indices: LDLc, HDLc, TG and Cholesterol (n=61, r2=0.14, P=0.003). For the same relationship between BMD and the composite lipid indices, a significant correlation was also obtained when the age of diagnosis was between 61 and 70 (n=201, r2=0.09, P<0.01). In this latter subgroup of patients, a significant correlation between BMD and LDLc, HDLc, TG and Cholesterol was seen whether the subjects had sustained a fracture (n=88, r2=0.14, P<0.01) or had not (n=113, r2=0.24, P=0.02). In the oldest group we studied, >70, we noted, when comparing the same indices, a significant correlation (n=247, r2=0.11, P<0.01) although the significance of the correlation was no longer present if this older group was studied according to whether or not a fracture event occurred (Table 2).

Table 2 The results of the multivariate linear regression between BMD and LDLc, HDLc, TG and Cholesterol

Correlations between BMD and BMI, in the entire cohort or in the subgroups based on previous fracture (present/absent), on age (under 50 years, 51–60 years, 61–70 years, over 70 years) or on age and previous fractures (8 subgroups) were not statistically significant (P>0.05).

Correlations based on prescribed drugs

There was no correlation in the cohort who were receiving statins (n=150) between BMD and LDLc, HDLc, TG and cholesterol (P<0.001, r2=0.06), as well as in the subgroup of patients with no previous fractures (P<0.001, r2=0.06). A weak correlation was found for patients with previous fractures (P<0.001, r2=0.19).

In agreement with the data for all patients, data for patients taking statins showed a weak correlation for patients with ages at diagnosis between 51 and 60 years. (P<0.001, r2=0.11) and more powerful correlations for patients between 61 and 70 years at diagnosis (P<0.001, r2=0.33) and patients older than 70 years at diagnosis (P<0.001, r2=0.76). The correlations based both on these age groups and previous fracture state remained significant (Table 3).

Table 3 The results of the multivariate linear regression between BMD and LDLc, HDLc, TG and Cholesterol for patients taking statins*

For patients not taking statins (n=364) the correlation between BMD and the lipid profile gave a minimal r value, although a significant P value was reached (P<0.001, r2=0.03). Similar results were obtained when these patients were grouped according to previous fracture state (P<0.001, r2<0.05). The subgroups of patients under 51 years at diagnosis or between 51 and 60 years at diagnosis showed no correlation, either as a whole or when grouped according to previous fracture state.

Patients not receiving statins who were diagnosed with osteoporosis at ages between 61 and 70 years showed a weak correlation (P<0.001, r2=0.11), which improved after grouping according to previous fracture state, especially for the women with no previous fractures (P<0.001, r2=0.29 and P=0.029, r2=0.52). A more powerful correlation was obtained for patients diagnosed after the age of 70 years (P<0.001, r2=0.28), correlation which remained significant for patients with no previous fractures (P=0.005, r2=0.52)

When taking into account only those receiving antiresorptive drugs (bisphosphonates or SERMS), the entire cohort presented a modest correlation between BMD and the circulating lipid profile (P=0.04, r2=0.30). After taking into account previous fracture history, correlations lost their significance. Yet again, by age subgroups, significant correlations were present for the women diagnosed with osteoporosis between the ages of 51 and 60 years (P=0.05, r2=0.23) and those diagnosed after the age of 70 years (P=0.02, r2=0.55). The correlations maintained their significance when taking into account previous fractures for each group (P=0.05, r2=0.29 and P=0.003, r2=0.55).

Taking into account patients who used antiresorptive drugs and grouping them according to previous fracture state and statin use, we discovered strong correlations for those who had no previous fractures and did not take statins (P<0.001, r2=0.72) and for those who had previous fractures and were prescribed statins (P<0.001, r2=0.87).

BMD and lipid fractions

A positive correlation between BMD and HDL-cholesterol in the entire cohort was significant (r=0.05, P<0.0001; figure 1). In the subgroup of patients who were under 51 years of age at diagnosis, there was an unexpected inverse correlation (r=−0.190, P<0.001).

Figure 1
figure 1

The trendline for all the patients, according to the linear regression between HDLc and Bone Mineral Density (r=0.05). This graph shows the presence of a modest correlation, which is what we expected out of our study, as HDLc should be just another piece of the lipid puzzle in osteoporosis.

Univariate linear regressions when other age subgroups were analyzed showed positive correlations for patients at an age at diagnosis between 51 and 60 years (r= 0.073, P<0.000 1), 61 and 70 years (r=0.164, P<0.0001) and for those over 70 years (r=0.103, P<0.000 1). Correlations for all the subjects who were diagnosed after reaching the age of 51 years were positive and significant (r=0.104, P<0.000 1; figure 2) (Table 4).

Figure 2
figure 2

The trendline for all the patients, according to the linear regression between HDLc and Bone Mineral Density (r=−0.104). This graph further substantiates the possibility of a link between Bone Mineral Density and the circulating lipid profile in women diagnosed after 50 years.

Table 4 The results of the univariate linear regression between BMD and HDLc.

When analyzing the correlations between BMD and LDLc in the entire cohort or its subgroups according to previous fractures, as well as according to age and/or previous fractures (15 correlations), we obtained only results that were not significant (P>0.05) and that presented no correlation (r2<0.04;). Similar results were obtained for correlations between BMD and TG (P>0.05) and BMD and total Cholesterol (P>0.05).


The results presented in this report provide support for an association between osteoporosis and the circulating lipid profile. Significant correlations between BMD and the lipid profile were demonstrated among certain demographically defined populations with an emphasis on patients older than 51 years.

Our results are supported by previous findings published in the last decade. Makovey et al have shown that BMD is significantly negatively correlated with total cholesterol and LDLc in postmenopausal women not receiving hormone replacing therapy (HRT) and with HDLc in postmenopausal women receiving HRT (24). Orozco et al have shown ‘that hyperlipidemia could be associated with osteoporosis’ (22), while Tankó et al, in a longitudinal study, which included postmenopausal women between 50–75 years, showed that an increase in serum cholesterol was associated with a decrease in spine BMD, irrespective of BMI (25). Uyama et al demonstrated a ‘significant correlation of plaque score with total cholesterol level and low total BMD’ (26). Not all studies have shown these correlations. For example, in the prospective study of Samelson et al (27) and the two crosssectional studies of Solomon et al (28) and Wu et al (29), such correlations could not be demonstrated.

Studies including only Caucasian subjects have primarily shown, as we have, a positive correlation between HDLc and BMD (30). Apart from ethnicity, a second possible confounder is represented by the existence of numerous genes shown to influence both HDLc and BMD through a pleiotropic effect (eg ABCG8, APOE, ESR1) (31). The results, in the subgroup analysis, if replicated in a larger study, would also suggest that statins protect patients with no history of previous fractures (P<0.001, r2=0.94).

A possible association between osteoporosis and the lipid profile could be explained at a molecular level as well: Franceschi described the ‘global reduction in the capacity to cope with a variety of stressors and a concomitant progressive increase in proinflammatory status’ (the “inflammaging” theory) which can lead to age related diseases such as osteoporosis (32). Another theory, based on the epidemiological link between hyperlipidemia, visceral obesity and osteoporosis, supports these findings through the ‘osteo-lipo-vascular interactions’ which exist due to the common origin of osteoblasts, vascular smooth muscle cells and adipocytes (33).

Limitations to this study include its retrospective design. In addition, a significant correlation for the entire patient cohort, as well as by subgroups according to previous fracture, was not demonstrable. While the lack of very strong correlations, albeit many with statistical significance, might be viewed as a limitation, one would not expect to find very high r values when only one piece of a pathogenic puzzle is identified in the context of a multifactorial disease like osteoporosis. As such, this new data sheds light on the plausible link between osteoporosis and the lipid profile, link, which if proven through prospective randomized studies would allow for a better treatment of patients with osteoporosis.

One of the main strengths of our study is that, in comparison to previous reports, we have stratified the subjects according to age. This is also one of the first clinical studies associating postmenopausal osteoporosis with the circulating lipid profile both with and without statin use and/or antiresorptive drug use, while taking into consideration age groups. As such, the analyses provide support for the idea that BMD may be associated with the entire lipid profile for postmenopausal women older than 50 years. Future studies may elucidate molecular or biochemical mechanisms for these observations.