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Cord blood attached segment: is this a relevant quality control to predict a good hematopoietic stem cell graft?

Bone Marrow Transplantation volume 52, pages 1353–1354 (2017)Cite this article

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Umbilical cord blood (UCB) is a source of hematopoietic stem cells (HSCs) used for allogeneic transplantation of patients with hematological diseases. Public cord blood banks (CBB) have been established worldwide to collect, cryopreserve and store UCB units for future transplantation.

UCB unit selection by a transplant center is primarily based on donor-recipient HLA compatibility and pre-freezing total nucleated cells (TNC) content. However, UCB units can be stored for long periods before their use for transplantation and potentially be exposed to temperatures outside the recommended range, resulting in an alteration of graft quality.1 CBBs must therefore establish a quality control to assess the quality of the UCB units before their release for transplantation. The use of an attached segment (AS) to the UCB unit, containing an aliquot of the cryopreserved product, is proposed as a quality control.2 This quality control consists of determining cell viability and/or clonogenic potential to confirm the effectiveness of the UCB unit selected for clinical use.3

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Acknowledgements

We gratefully thank Eurocord for providing the Saint-Louis Hospital UCB graft data. We thank also France Greffe de Moelle for their work that permitted to collect all the UCB thawing data.

Author contributions

LF/AC conceived, designed the study, acquired the data, interpreted the results, drafted and approved the final version. HB conceived and designed the study, acquired the data, interpreted results and approved the final version. RZ acquired the data and interpreted the results. TD/AD approved the final version. CC revised the manuscript. JL/VV approved and revised the manuscript.

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Authors and Affiliations

  1. AP-HP, Hôpital Saint-Louis, Unité de Thérapie Cellulaire, Paris, France

    L Faivre, H Boucher, T Domet, A Desproges, C Couzin, V Vanneaux, J Larghero & A Cras

  2. Université Paris Descartes, Sorbonne Paris Cité, Paris, France

    H Boucher, R Zerbib & A Cras

  3. Inserm, UMR_S1160, Centre d’Investigation Clinique en Biothérapies, Paris, France

    V Vanneaux & J Larghero

  4. Université Paris Diderot, Sorbonne Paris Cité, Paris, France

    J Larghero

  5. Inserm UMR_S1140, Faculté de Pharmacie, Paris, France

    A Cras

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  1. L Faivre
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Correspondence to A Cras.

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Faivre, L., Boucher, H., Zerbib, R. et al. Cord blood attached segment: is this a relevant quality control to predict a good hematopoietic stem cell graft?. Bone Marrow Transplant 52, 1353–1354 (2017). https://doi.org/10.1038/bmt.2017.150

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