This is a preview of subscription content, access via your institution
Subscribe to Journal
Get full journal access for 1 year
only $9.92 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Get time limited or full article access on ReadCube.
All prices are NET prices.
Rivera GK, Pinkel D, Simone JV, Hancock ML, Crist WM . Treatment of acute lymphoblastic leukemia: 30 years’ experience at St Jude Children’s Research Hospital. New Engl J Med 1993; 329: 1289–1295.
Kiehl MG, Kraut L, Schwerdtfeger R, Hertenstein B, Remberger M, Nicolaus K et al. Outcome of allogeneic hematopoietic stem-cell transplantation in adult patients with acute lymphoblastic leukemia: no difference in related compared with unrelated transplant in first complete remission. J Clin Oncol 2004; 22: 2816–2825.
Formánková R, Sedlácek P, Krsková L, Ríhová H, Srámková L, Star J et al. Chimerism-directed adoptive immunotherapy in the prevention and treatment of post-transplant relapse of leukemia in childhood. Haematologica 2003; 88: 117–118.
Dey BR, McAfee S, Colby C, Sackstein R, Saidman S, Tarbell N et al. Impact of prophylactic donor leukocyte infusions on mixed chimerism, graft-versus-host disease, and antitumor response in patients with advanced hematologic malignancies treated with nonmyeloablative conditioning and allogeneic bone marrow transplantation. Biol Blood Marrow Transplant 2003; 9: 320–329.
Bader P, Kreyenberg H, Hoelle W, Dueckers G, Handgretinger R, Lang P et al. Increasing mixed chimerism is an important prognostic factor for unfavorable outcome in children with acute lymphoblastic leukemia after allogeneic stem-cell transplantation: possible role for pre-emptive immunotherapy? J Clin Oncol 2004; 22: 1696–1706.
Bader P, Beck J, Frey A, Schlegel PG, Hebarth H, Handgretinger R et al. Serial and quantitative analysis of mixed hematopoietic chimerism by PCR in patients with acute leukemias allows the prediction of relapse after allogeneic BMT. Bone Marrow Transplant 1998; 21: 487–495.
Casarino L, Carbone C, Capucci MA, Izzi T, Ferrara GB . Analysis of chimerism after bone marrow transplantation using specific oligonucleotide probes. Bone Marrow Transplant 1992; 10: 165–170.
Oliver DH, Thompson RE, Griffin CA, Eshleman JR . Use of single nucleotide polymorphisms (SNP) and real-time polymerase chain reaction for bone marrow engraftment analysis. J Mol Diagn 2000; 2: 202–208.
Alizadeh M, Bernard M, Danic B, Dauriac C, Birebent B, Lapart C et al. Quantitative assessment of hematopoietic chimerism after bone marrow transplantation by real-time quantitative polymerase chain reaction. Blood 2002; 99: 4618–4625.
Maas F, Schaap N, Kolen S, Zoetbrood A, Buño I, Dolstra H et al. Quantification of donor and recipient hemopoietic cells by realtime PCR of single nucleotide polymorphisms. Leukemia 2003; 17: 621–629.
Weber JL, David D, Heil J, Fan Y, Zhao C, Marth G . Human diallelic insertion/deletion polymorphisms. Am J Hum Genet 2002; 71: 854–862.
Wilhelm J, Reuter H, Tews B, Pingoud A, Hahn M . Detection and quantification of insertion/deletion variations by allele-specific real-time PCR: application for genotyping and chimerism analysis. Biol Chem 2002; 383: 1423–1433.
Jiménez-Velasco A, Barrios M, Román-Gómez J, Navarro G, Buño I, Castillejo JA et al. Reliable quantification of hematopoietic chimerism after allogeneic transplantation for acute leukemia using amplification by real-time PCR of null alleles and insertion/deletion polymorphisms. Leukemia 2005; 19: 336–343.
Heid CA, Stevens J, Livak KL, Williams PM . Real time quantitative PCR. Genome Res 1996; 6: 986–994.
Vicente D, Podestà M, Pitto A, Pozzi S, Lucchetti S, Lamparelli T et al. Progenitor cells trapped in marrow filters can reduce GvHD and transplant mortality. Bone Marrow Transplant 2006; 38: 111–117.
We would like to thank São Paulo Research Foundation for funding this research and the Laboratory of Molecular Analysis of Polymorphism of the University of Campinas for providing the DNA samples.
The authors declare no conflict of interest.
Supplementary Information accompanies this paper on Bone Marrow Transplantation website
About this article
Cite this article
Vicente, D., Laranjeira, A., Miranda, E. et al. Chimerism interpretation with a highly sensitive quantitative PCR method: 6 months median latency before chimerism drop below 0.1%. Bone Marrow Transplant 51, 874–875 (2016). https://doi.org/10.1038/bmt.2016.5