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Digital-PCR assay for screening and quantitative monitoring of calreticulin (CALR) type-2 positive patients with myelofibrosis following allogeneic stem cell transplantation

Bone Marrow Transplantation volume 51, pages 872–873 (2016)Cite this article

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Allogeneic stem cell transplantation (aSCT) is a curative treatment approach for patient with myelofibrosis. Monitoring patients after aSCT using molecular disease markers has become a valid tool to guide adoptive immunotherapy with donor lymphocyte infusion in chronic myeloid leukemia and other diseases.1 In particular, myelofibrosis-specific mutations such as JAK2V617F (~50% of patients) and MPLW515L and MPLW515K (~5%) have successfully been used for post-aSCT minimal residual disease (MRD) monitoring.2,3,4,5

The recently discovered somatically acquired calreticulin (CALR) mutations in ~30% of all and 80% of the Janus kinase 2 (JAK2)- and myeloproliferative leukemia protein (MPL)-negative myelofibrosis patients6,7,8 provide new diagnostic markers, which can also be used as indicators of MRD following curative treatment with aSCT.

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Acknowledgements

MC has been supported by the Erich und Gertrud Roggenbuck-Stiftung.

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Author notes

  1. A Badbaran and B Fehse: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, Germany

    A Badbaran, B Fehse, M Christopeit, T Aranyossy, F A Ayuk, C Wolschke & N Kröger

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  1. A Badbaran
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  2. B Fehse
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  4. T Aranyossy
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  7. N Kröger
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Corresponding authors

Correspondence to B Fehse or N Kröger.

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Supplementary Information accompanies this paper on Bone Marrow Transplantation website

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Badbaran, A., Fehse, B., Christopeit, M. et al. Digital-PCR assay for screening and quantitative monitoring of calreticulin (CALR) type-2 positive patients with myelofibrosis following allogeneic stem cell transplantation. Bone Marrow Transplant 51, 872–873 (2016). https://doi.org/10.1038/bmt.2016.14

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