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Infusion of cryopreserved autologous lymphocytes using a standard peripheral i.v. catheter

Bone Marrow Transplantation volume 49, pages 1119–1120 (2014)Cite this article

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Cryopreserved hematopoietic progenitor cell (HPC) components are generally administered via a central venous catheter (CVC). As most patients receiving these products have an established central venous line, alternative administration routes have not been carefully explored. Here, we report the first series of patients receiving cryopreserved, thawed lymphocytes using peripheral i.v. lines. Newly diagnosed adult, high-grade glioma patients underwent lymphocyte collection before starting radiation with reinfusion after completing radiation. Reinfusion was performed using a temporary 20-gauge peripheral i.v. line. Patients were observed during and after infusion for toxicity. Ten patients underwent a 2-h lymphocyte collection and eight underwent lymphocyte reinfusion. The median cryopreserved product composition contained a volume of 60 cm3, 3 mL of DMSO, a median of 9.9 × 107 nucleated cells/kg and a median of 5.9 × 107 CD3+ cells/kg. No toxicities of any kind occurred during or following the peripheral infusion of thawed lymphocytes. This feasibility study in patients with high-grade gliomas provided a unique opportunity to assess the frequency and severity of patient reactions associated with peripheral venous infusions of small volumes of cryopreserved, thawed lymphocytes. The lack of any notable toxicity suggests that patients without central venous access could receive similar products via a peripheral i.v. line.

DMSO dissipates the osmotic stress that would otherwise result in leukocyte lysis during cryopreserving HPCs.1, 2, 3 The presence of DMSO and granulocytes dominate the adverse events (AEs) associated with cryopreserved cellular therapy (CT) infusions.2,4,5 The DMSO infusion-related AEs are minimalized by limiting receipts to <1 g/kg of DMSO per day, acetaminophen, histamine blockade and CT infusion via a CVC.1,2,6

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Acknowledgements

Many thanks are extended to our aphesis staff and graft engineering staff who made this work possible.

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Authors and Affiliations

  1. The Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA

    D E Gladstone, J Davis-Sproul, J Campian, M V Lemas, S Malatyali, I Borrello, K King & S A Grossman

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  1. D E Gladstone
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  2. J Davis-Sproul
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  3. J Campian
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  4. M V Lemas
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  5. S Malatyali
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  6. I Borrello
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  7. K King
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  8. S A Grossman
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Correspondence to D E Gladstone.

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Gladstone, D., Davis-Sproul, J., Campian, J. et al. Infusion of cryopreserved autologous lymphocytes using a standard peripheral i.v. catheter. Bone Marrow Transplant 49, 1119–1120 (2014). https://doi.org/10.1038/bmt.2014.98

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