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Cell Selection

Donor-derived CD4+/CCR7+ T-cell partial selective depletion does not alter acquired anti-infective immunity

Bone Marrow Transplantation volume 49, pages 611–615 (2014)Cite this article

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Abstract

In previous studies, we observed that a high proportion of donor-derived CD4+ T cells expressing the chemokine receptor 7 (CCR7) was a major determinant of acute GVHD, without interfering with the incidence of other post-transplant outcomes, especially relapse and nonrelapse mortality rates. Here, we investigated in vitro the impact of partially selective CD4+/CCR7+ T lymphocytes on acquired anti-infective immune response in 10 donors who underwent G-CSF-primed PBSC collection. Similar quantitative and functional proliferative reactions were observed in lymphocyte cultures in the presence of adenovirus and pp65 Ags with unmanipulated and partially depleted donor samples. No responses were observed in the presence of human T-cell lymphotropic virus type 1 used as a negative control. These results complete the proof of concept needed to build a clinical trial investigating partially selective CD4+/CCR7+ T cell-depleted allo-SCT.

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Acknowledgements

We thank all the donors who accepted to participate in this research and the members of the association Capucine for their generous support to our clinical and basic research work. This study has been entirely conducted with a donation from the Association Capucine.

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Authors and Affiliations

  1. Service d’hématologie, CH, Boulogne Sur Mer, France

    B Choufi

  2. EA2686, Université de Lille 2, Lille, France

    B Choufi, J Trauet, S Thiant, M Labalette & I Yakoub-Agha

  3. Laboratoire d’Immunologie, CHRU, Lille, France

    J Trauet, S Thiant & M Labalette

  4. Service des Maladies du Sang, CHRU LILLE, Bd Jules Leclercq, UAM allogreffes de CSH, Lille, France

    I Yakoub-Agha

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Correspondence to I Yakoub-Agha.

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Author contributions

BC, JT and ST contributed to analysis and wrote the manuscript; ML designed and supervised the study, analyzed data and wrote the manuscript. IY-A designed and supervised the study, enrolled the donors, analyzed data and wrote the manuscript.

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Choufi, B., Trauet, J., Thiant, S. et al. Donor-derived CD4+/CCR7+ T-cell partial selective depletion does not alter acquired anti-infective immunity. Bone Marrow Transplant 49, 611–615 (2014). https://doi.org/10.1038/bmt.2014.6

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