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Successful second autologous engraftment after long duration storage of hematopoietic stem cells

Bone Marrow Transplantation volume 48, pages 1480–1481 (2013)Cite this article

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We read with great interest the article by Fernyhough et al.1 about the recovery of hematopoietic stem cells (HSCs) after extended cryostorage. As successful engraftment of HSCs depends on several factors related to the HSC collection itself (CD34+ cell count, cryopreservation techniques and constant temperature monitoring) and to the receiving host (myeloablative chemotherapy, BM microenvironment and therapeutic history), we want to report our scientific and clinical experience in this field, adding some comments about the currently used management of cryosystems. Studies by other groups about the relationship between cryostorage duration and hematopoietic engraftment potential have been performed mostly with in vitro assays on cord blood cells2 or, in vivo, using only BM-derived HSCs cryostored for a limited amount of time.3, 4 In addition, in the latter reports, data are available only for patients engrafted once, taking as a reference for hematological recovery the accepted time frame for BM reconstitution;5 these studies, although reliable, lack an internal control to compare the engraftment potential of hematopoietic stem cells after a short and a long period of cryostorage.

We want to report data from two of our patients engrafted successfully twice, each time after myeloablative conditioning. These data come from a survey performed in our database on more than 400 transplanted, consecutive and unselected patients; in addition, the same search was not able to identify any graft failure. In both cases, HSCs harvested from peripheral blood were cryopreserved and reinfused for the first time after a few months and after a cryostorage lasting about 4 and 10 years the second time. As for clinical practice and European Group for Blood and Marrow Transplantation (EBMT) guidelines, hematopoietic growth factors (that is, G-CSF, 300 μg/day, subcutaneously) were administered after each reinfusion until WBC count reached 5.0 × 103/μL. Both patients gave written informed consent before myeloablative therapy.

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Author notes

  1. P Longoni and M Milanesi: These authors contributed equally to this work.

Authors and Affiliations

  1. Cryopreservation Laboratory, Fondazione IRCCS, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy

    P Longoni, M Milanesi & P Corradini

  2. Division of Medical Oncology, Bone Marrow Transplantation Unit, Fondazione IRCCS, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy

    M Di Nicola, L Devizzi, A Gianni & M Magni

  3. Department of Oncology, Hematology and BMT Unit, San Raffaele Scientific Institute, Milan, Italy

    M Carrabba

  4. Transfusion Medicine Service, Apheresis Unit, Fondazione IRCCS, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy

    F Arienti & F Ravagnani

  5. S.C.D.U. Hematology and Cellular Therapy, A.O. Ordine Mauriziano-Umberto I, Turin, Italy

    C Tarella

  6. Division of Hematology and Bone Marrow Transplantation, Fondazione IRCCS, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy

    V Montefusco & P Corradini

  7. University of Milan, Milan, Italy

    A Gianni

  8. Department of Hematology, University of Milan, Milan, Italy

    P Corradini

Authors
  1. P Longoni
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  2. M Milanesi
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  3. M Di Nicola
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  4. L Devizzi
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  5. M Carrabba
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  6. F Arienti
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  7. F Ravagnani
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  8. C Tarella
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  9. V Montefusco
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  10. A Gianni
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  11. P Corradini
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  12. M Magni
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Corresponding author

Correspondence to M Magni.

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The authors declare no conflict of interest.

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Longoni, P., Milanesi, M., Di Nicola, M. et al. Successful second autologous engraftment after long duration storage of hematopoietic stem cells. Bone Marrow Transplant 48, 1480–1481 (2013). https://doi.org/10.1038/bmt.2013.81

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