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Microbial Issues

Human parvoviruses B19, PARV4 and bocavirus in pediatric patients with allogeneic hematopoietic SCT

Bone Marrow Transplantation volume 48, pages 1308–1312 (2013)Cite this article

Abstract

Among the immunocompetent, infections with parvovirus B19 (B19V) and human bocavirus (HBoV) 1 range clinically from asymptomatic to severe, while following allogeneic hematopoietic SCT (HSCT) B19V can cause a persistent severe illness. The epidemiology and clinical impact of HBoV1 and the other emerging parvovirus 4 (PARV4) among immunocompromised patients have not been established. To determine the occurrence and clinical spectrum of B19V, PARV4 and HBoV1 infections, we performed a longitudinal molecular surveillance among 53 allogeneic HSCT recipients for pre- and post-HSCT DNAemias of these parvoviruses. Quantitative real-time PCR showed B19V DNA in sera of 16 (30%) patients, at mean levels of 4.6 × 103, 9.9 × 107, 1.1 × 1010 and 1.6 × 102 B19V DNA copies/mL pre-HSCT (9/53), and at 1 (6/53), 2 (4/53) and 3 months (1/25) post HSCT, respectively. However, no clinical manifestation correlated with the presence of B19V viremia. All B19V sequences were of genotype 1. None of the sera investigated contained PARV4 or HBoV1 DNAs. Our data demonstrate B19V viremia to be frequent among pediatric allogeneic HSCT recipients, yet without apparent clinical correlates. PARV4 or HBoV1 viremias were not seen in these immunocompromised patients.

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Acknowledgements

This work was supported by grants from the Foundation for Pediatric Research, the Nona and Kullervo Väre Foundation, the University of Helsinki Research Fund, and the Turku University Foundation, the Sigrid Jusélius Foundation, the Finnish Medical Foundation (FLS), and the Academy of Finland (Grant No. 1257964).

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Authors and Affiliations

  1. Division of Pediatric Hematology, Oncology and Stem Cell Transplantation, Children's Hospital, Helsinki University Central Hospital and University of Helsinki, Helsinki, Finland

    J Rahiala, M Koskenvuo & U Saarinen-Pihkala

  2. Department of Pediatrics, Porvoo Hospital, Porvoo, Finland

    J Rahiala

  3. Department of Pediatrics, University of Turku, Turku, Finland

    M Koskenvuo & O Ruuskanen

  4. Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland

    P Norja, M Meriluoto, M Toppinen, A Lahtinen, E Väisänen, K Hedman & M Söderlund-Venermo

  5. Department of Virology, University of Turku, Turku, Finland

    M Waris & T Vuorinen

  6. Department of Virology and Immunology, Helsinki University Central Hospital Laboratory Division (HUSLAB), Helsinki, Finland

    M Lappalainen & K Hedman

  7. Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden

    T Allander

  8. Division of Hematology-Oncology, Department of Pediatrics, University of Tampere, Tampere, Finland

    K Vettenranta

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Rahiala, J., Koskenvuo, M., Norja, P. et al. Human parvoviruses B19, PARV4 and bocavirus in pediatric patients with allogeneic hematopoietic SCT. Bone Marrow Transplant 48, 1308–1312 (2013). https://doi.org/10.1038/bmt.2013.63

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