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Allostimulatory activity of CD133+ hematopoietic cells

Bone Marrow Transplantation volume 48, pages 742–744 (2013)Cite this article

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The CD133 receptor is expressed on most, but not all CD34+ hematopoietic stem cells and endothelial progenitor cells.1 Human CD133+ cells include committed progenitors, as well as early precursors, with in vivo repopulating activity in NOD/SCID mice.2 Moreover, CD133+ cells have been successfully used in clinical trials of allogeneic hematopoeitic SCT.3, 4

Then, we tested whether the stimulation of progenitor cells with allo-T cells for 1 or 24 h would affect the clonogenic activity (CFU-C)8 of CD34+ or CD133+ cells in vitro (Figure 1c). In the absence of T cells, CD133+ cells generated a moderately smaller number of erythroid colonies (BFU-E) compared with CD34+ cells (P=0.1, n=3 experiments). When CD133+ cells were stimulated with autologous or allogeneic T cells for 24 h the number of BFU-E significantly increased (P=0.01) (n=5 experiments). On the contrary, stimulation with T cells did not modify the number of erythroid colonies in cultures with CD34+ cells. No effect of priming with T cells was observed in other lineages.

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References

  1. Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997; 90: 5002–5012.

    CAS  PubMed  Google Scholar 

  2. de Wynter EA, Buck D, Hart C, Heywood R, Coutinho LH, Clayton A et al. CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. Stem Cells 1998; 16: 387–396.

    Article  CAS  PubMed  Google Scholar 

  3. Bitan M, Shapira MY, Resnick IB, Zilberman I, Miron S, Samuel S et al. Successful transplantation of haploidentically mismatched peripheral blood stem cells using CD133+-purified stem cells. Exp Hematol 2005; 33: 713–718.

    Article  CAS  PubMed  Google Scholar 

  4. Lang P, Bader P, Schumm M, Feuchtinger T, Einsele H, Fuhrer M et al. Transplantation of a combination of CD133+ and CD34+ selected progenitor cells from alternative donors. Br J Haematol 2004; 124: 72–79.

    Article  PubMed  Google Scholar 

  5. Rondelli D, Andrews RG, Hansen JA, Ryncarz R, Faerber MA, Anasetti C . Alloantigen presenting function of normal human CD34+ hematopoietic cells. Blood 1996; 88: 2619–2625.

    CAS  PubMed  Google Scholar 

  6. Rondelli D, Anasetti C, Fortuna A, Ratta M, Arpinati M, Bandini G et al. T-cell alloreactivity induced by normal G-CSF-mobilized CD34+ blood cells. Bone Marrow Transplant 1998; 21: 1183–1191.

    Article  CAS  PubMed  Google Scholar 

  7. Chunduri S, Mahmud D, Abbasian J, Arpinati M, Rondelli D . Cord blood cells induce delayed T cell alloreactivity. Biol Blood Marrow Transplant 2008; 14: 872–879.

    Article  PubMed  Google Scholar 

  8. Rondelli D, Lemoli RM, Ratta M, Fogli M, Re F, Curti A et al. Rapid induction of CD40 on a subset of granulocyte colony-stimulating factor-mobilized CD34(+) blood cells identifies myeloid committed progenitors and permits selection of nonimmunogenic CD40(−) progenitor cells. Blood 1999; 94: 2293–2300.

    CAS  PubMed  Google Scholar 

  9. Abbasian J, Mahmud D, Mahmud N, Chunduri S, Araki H, Reddy P et al. Allogeneic T cells induce rapid CD34+ cell differentiation into CD11c+CD86+ cells with direct and indirect antigen-presenting function. Blood 2006; 108: 203–208.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Bonetti MI, Pieri L, Domenici L, Urbani S, Romano G, Aldinucci A et al. Dendritic cells with lymphocyte stimulating activity differentiate from human CD133 positive precursors. Blood 2011; 117: 3983–3995.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Section of Hematology/Oncology, University of Illinois Hospital & Health Sciences System, Chicago, IL, USA

    P Patel, J Abbasian, D Mahmud, N Mahmud, P M Horsthemke, S Chunduri & D Rondelli

  2. Cancer Center, University of Illinois, Chicago, IL, USA

    P Patel, N Mahmud & D Rondelli

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  1. P Patel
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  2. J Abbasian
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  3. D Mahmud
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  4. N Mahmud
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  5. P M Horsthemke
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  6. S Chunduri
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  7. D Rondelli
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Correspondence to D Rondelli.

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Patel, P., Abbasian, J., Mahmud, D. et al. Allostimulatory activity of CD133+ hematopoietic cells. Bone Marrow Transplant 48, 742–744 (2013). https://doi.org/10.1038/bmt.2012.212

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