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Mobilization and harvesting of PBPC in newly diagnosed type 1 diabetes mellitus

Bone Marrow Transplantation volume 47, pages 993–994 (2012)Cite this article

Autologous hematopoietic stem cell (HSC) transplantation is an established treatment for hematological malignancies, and recently has been employed to treat severe chronic autoimmune diseases (CAID),1 including newly diagnosed type 1 diabetes mellitus (T1DM).2 The objective of this modality of treatment in autoimmune diseases is the elimination of auto-reactive clones of T cells to allow the regeneration of a ‘new’ immune system from the HSC infused. At diagnosis most of β-cell mass has been destroyed. However, blocking autoimmune aggression could spare the remaining β-cells and confer a clinical remission.2

Mobilization and harvesting of HSC from PBSC is an essential part of autologous transplantation, but the optimal strategy for it remains unclear. Usually, HSC mobilization includes administration of G-CSF, alone or in combination with chemotherapy,3 to harvest a minimum of 2.0 × 106 CD34+ cells/kg for rapid engraftment after a myeloablative conditioning regimen. Nevertheless, it is estimated that 2–40% of patients fail to achieve the minimal cellular dose for transplant.4

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Acknowledgements

We thank Aline CG Silva, Natalia C Gonçalves and Camila COM Bonaldo for the laboratory assistance and to the Apheresis Section and Bone Marrow Transplantation Unit team.

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Authors and Affiliations

  1. National Institute of Science and Technology in Stem Cell and Cell Therapy, Center for Cell Therapy and Regional Blood Center, Ribeirao Preto, Brazil

    G C De Santis, B de Pina Almeida Prado Jr, K de Lima Prata, D M Brunetta, M D Orellana, P V B Palma, J C Voltarelli & D T Covas

  2. Department of Internal Medicine, School of Medicine, University of Sao Paulo, Ribeirao Preto, Brazil

    G C De Santis, B de Pina Almeida Prado Jr, K de Lima Prata, D M Brunetta, M D Orellana, B P Simoes, J C Voltarelli & D T Covas

  3. Bone Marrow Transplantation Unit, Hospital das Clinicas, School of Medicine, University of Sao Paulo, Ribeirao Preto, Brazil

    M C Oliveira, B P Simoes & J C Voltarelli

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  1. G C De Santis
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  2. B de Pina Almeida Prado Jr
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  10. D T Covas
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Correspondence to G C De Santis.

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De Santis, G., de Pina Almeida Prado, B., de Lima Prata, K. et al. Mobilization and harvesting of PBPC in newly diagnosed type 1 diabetes mellitus. Bone Marrow Transplant 47, 993–994 (2012). https://doi.org/10.1038/bmt.2011.188

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