Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Graft-Versus-Host Disease

Expression of CD64 (FcγRI) in skin of patients with acute GVHD

Abstract

GVHD remains a major problem in allo-SCT. We explored the presence of APC in skin biopsies of GVHD patients, using the IgG receptor CD64 expression as a hallmark for activated APC. By immunohistochemistry we demonstrated CD64 to be upregulated on host APC in skin biopsies of patients with acute GVHD and, less prominently, in chronic GVHD. Double staining for CD32 polymorphism revealed CD64-positive cells to be mainly of host origin. The majority of CD64-positive cells coexpressed CD68, indicating a macrophage phenotype. Given its very restricted cellular distribution, CD64 may represent an excellent target for APC-directed therapies in GVHD.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2

Similar content being viewed by others

References

  1. Shlomchik WD . Graft-versus-host disease. Nat Rev Immunol 2007; 7: 340–352.

    Article  CAS  PubMed  Google Scholar 

  2. Duffner UA, Maeda Y, Cooke KR, Reddy P, Ordemann R, Liu C et al. Host dendritic cells alone are sufficient to initiate acute graft-versus-host disease. J Immunol 2004; 172: 7393–7398.

    Article  CAS  PubMed  Google Scholar 

  3. Shlomchik WD, Couzens MS, Tang CB, McNiff J, Robert ME, Liu J et al. Prevention of graft versus host disease by inactivation of host antigen-presenting cells. Science 1999; 285: 412–415.

    CAS  PubMed  Google Scholar 

  4. Merad M, Hoffmann P, Ranheim E, Slaymake S, Manz MG, Lira SA et al. Depletion of host Langerhans cells before transplantation of donor alloreactive T cells prevents skin graft-versus-host disease. Nat Med 2004; 10: 510–517.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Ball LM, Egeler RM . Acute GvHD: pathogenesis and classification. Bone Marrow Transplant 2008; 41 (Suppl 2): S58–S64.

    Article  CAS  PubMed  Google Scholar 

  6. Haniffa M, Ginhoux F, Wang XN, Bigley V, Abel M, Dimmick I et al. Differential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation. J Exp Med 2009; 206: 371–385.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Cassatella MA, Flynn RM, Amezaga MA, Guasparri I, Rossi F, Trinchieri G . Interferon gamma induces in human neutrophils and macrophages expression of the mRNA for the high affinity receptor for monomeric IgG (Fc gamma R-I or CD64). Biochem Biophys Res Commun 1990; 170: 582–588.

    Article  CAS  PubMed  Google Scholar 

  8. te Velde AA, de Waal Malefijt R, Huijbens RJ, de Vries JE, Figdor CG . IL-10 stimulates monocyte Fc gamma R surface expression and cytotoxic activity. Distinct regulation of antibody-dependent cellular cytotoxicity by IFN-gamma, IL-4, and IL-10. J Immunol 1992; 149: 4048–4052.

    CAS  PubMed  Google Scholar 

  9. Beekman JM, Bakema JE, van de Winkel JGJ, Leusen JHW . Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity. Proc Natl Acad Sci USA 2004; 101: 10392–10397.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Goker H, Haznedaroglu IC, Chao NJ . Acute graft-vs-host disease: pathobiology and management. Exp Hematol 2001; 29: 259–277.

    Article  CAS  PubMed  Google Scholar 

  11. Lerner KG, Kao GF, Storb R, Buckner CD, Clift RA, Thomas ED . Histopathology of graft-vs.-host reaction (GvHR) in human recipients of marrow from HL-A-matched sibling donors. Transplant Proc 1974; 6: 367–371.

    CAS  PubMed  Google Scholar 

  12. Gosselin EJ, Brown MF, Anderson CL, Zipf TF, Guyre PM . The monoclonal antibody 41H16 detects the Leu 4 responder form of human Fc gamma RII. J Immunol 1990; 144: 1817–1822.

    CAS  PubMed  Google Scholar 

  13. van Royen-Kerkhof A, Sanders EA, Wijngaarden S, van Roon JA, Voorhorst-Ogink M, Walraven V et al. Flow cytometric determination of FcgammaRIIa (CD32) polymorphism. J Immunol Methods 2004; 294: 135–144.

    Article  CAS  PubMed  Google Scholar 

  14. Carlsson LE, Santoso S, Baurichter G, Kroll H, Papenberg S, Eichler P et al. Heparin-induced thrombocytopenia: new insights into the impact of the FcgammaRIIa-R-H131 polymorphism. Blood 1998; 92: 1526–1531.

    CAS  PubMed  Google Scholar 

  15. Thepen T, van Vuuren AJ, Kiekens RC, Damen CA, Vooijs WC, van de Winkel JGJ . Resolution of cutaneous inflammation after local elimination of macrophages. Nat Biotechnol 2000; 18: 48–51.

    Article  CAS  PubMed  Google Scholar 

  16. van Roon J, Wijngaarden S, Lafeber FP, Damen C, van de Winkel JGJ, Bijlsma JW . Interleukin 10 treatment of patients with rheumatoid arthritis enhances Fc gamma receptor expression on monocytes and responsiveness to immune complex stimulation. J Rheumatol 2003; 30: 648–651.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J H W Leusen.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Cite this article

van Royen-Kerkhof, A., Walraven, V., Sanders, E. et al. Expression of CD64 (FcγRI) in skin of patients with acute GVHD. Bone Marrow Transplant 46, 1566–1569 (2011). https://doi.org/10.1038/bmt.2010.337

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/bmt.2010.337

Keywords

Search

Quick links