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Autografting

Relationship between TNFA, TNFB and TNFRII gene polymorphisms and outcome after unrelated hematopoietic cell transplantation in a Chinese population

Bone Marrow Transplantation volume 46, pages 400–407 (2011)Cite this article

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Abstract

This study aimed to analyze the association between cytokine gene polymorphisms and outcome following allogeneic hematopoietic SCT (allo-HSCT). A total of 138 unrelated donor/recipient pairs who underwent allo-HSCT from 2001 to 2009 were tested for TNFA-1031 (T>C), -863 (C>A), -857 (C>T), -238 (G>A), TNFB+252 (A>G) and TNFRII codon 196 (T>G) single nucleotide polymorphisms by multiplex SnaPshot analysis. Transplantation involving recipients and/or donors with TNFA-857 C/C genotype or TNFB+252 G allele-positivity resulted in a higher incidence of acute GVHD (aGVHD), which was independent of HLA mismatching. In multivariate analysis, TNFA-857 C/C genotype donors (relative risk (RR)=2.29, P=0.006) and TNFB+252 G allele-positive recipients (RR=1.789, P=0.036) were found to be significantly associated with an increased incidence of aGVHD. TNFA-857 C/C genotype donors (RR=3.748, P=0.002) and TNFB+252 G allele-positive recipients (RR=1.823, P=0.063) were also associated with the development of grades II–IV aGVHD. TNFRII polymorphism in recipients was also related to relapse rate, but no significant associations were found between TNFA, TNFB or TNFRII 196 genotype and cGVHD, relapse or overall survival after transplantation. These results provide the first report of an association between TNFA, TNFB and TNFRII polymorphic features and outcome of allo-HSCT in a Chinese population, and suggest an interaction between TNFA-857 and TNFB+252 genotypes and risk of aGVHD.

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Acknowledgements

We thank Shanghai Genesky Bio-Tech Genetic Core Laboratory for their excellent technical assistance with genotyping analyses. We also thank the assistance of HLA Typing Laboratory of Blood Center of Zhejiang Province. This work was funded by Zhejiang Provincial Key Medical Discipline (Medical Tissue Engineering), Major Program of Zhejiang Provincial Science (2006C13022) and Health Foundation of Ministry of Public Health (200802027).

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  1. H W Xiao and X Y Lai: These authors contributed equally to this work.

Authors and Affiliations

  1. Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China

    H W Xiao, X Y Lai, Y Luo, J M Shi, Y M Tan, J S He, W Z Xie, L Li, X L Zhu, J J Zhu, J Sun, G Q Wei, L Jin, L Z Liu, K N Wu, X H Yu, Z Cai, M F Lin, X J Ye & H Huang

  2. Department of Haematology, Guangzhou Liuhuaqiao Hospital, Guangzhou, Guangdong Province, China

    H W Xiao

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Xiao, H., Lai, X., Luo, Y. et al. Relationship between TNFA, TNFB and TNFRII gene polymorphisms and outcome after unrelated hematopoietic cell transplantation in a Chinese population. Bone Marrow Transplant 46, 400–407 (2011). https://doi.org/10.1038/bmt.2010.135

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