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Pediatric Transplants

Cellular immune parameters associated with spontaneous control of CMV in children who underwent transplantation

Bone Marrow Transplantation volume 45, pages 442–449 (2010)Cite this article

Abstract

CD4+ T-cell functions that best correlate with CMV control were evaluated by studying the relationship between CMV infection and CMV-specific immune recovery as determined by proliferation assay and intracytoplasmic-IFNγ assay. A total of 30 children (mean age: 8.30 years) who received an allogeneic hematopoietic SCT (HSCT) were included. In total, 13 recipients were seronegative before HSCT. None developed CMV infection or CMV-specific immunity. A total of 17 recipients were seropositive: (i) four patients spontaneously controlled CMV. The median of CMV-specific IFNγ-secreting CD4 T cells was 9.13/μl at month 3 in these four patients and three of the four patients evidenced optimal proliferative responses since month 1; (ii) in 10 patients who received anti-CMV chemotherapy because of prolonged viremia, lower (P=0.016) IFNγ responses (0.39/μl), together with delayed and/or depressed proliferative responses, were observed; (iii) finally, one patient with early CMV-associated disease had undetectable proliferative and IFNγ responses until month 3. In conclusion, both intense IFNγ responses and early proliferative responses seem to be associated with optimal CMV control.

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Acknowledgements

We thank the clinical team for patient care, Guylaine Boiry, Anne-Marie Courchinoux, Elodie Geneletti and Ingrid Hamon for excellent technical assistance and Céline Neto for typing the manuscript and drawing the figures. This work was supported by Assistance Publique—Hôpitaux de Paris and University Paris VII, France.

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Authors and Affiliations

  1. Laboratory of Immunology, Assistance Publique-Hôpitaux de Paris, Hôpital Robert Debré, Université Paris VII, Paris, France

    V Guérin, B Pédron & G Sterkers

  2. Department of Hematology, Assistance Publique-Hôpitaux de Paris, Hôpital Robert Debré, Université Paris VII, Paris, France

    J-H Dalle, M Ouachée-Chardin, K Yakouben & A Baruchel

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  1. V Guérin
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Correspondence to G Sterkers.

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Guérin, V., Dalle, JH., Pédron, B. et al. Cellular immune parameters associated with spontaneous control of CMV in children who underwent transplantation. Bone Marrow Transplant 45, 442–449 (2010). https://doi.org/10.1038/bmt.2009.179

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