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α4-integrin-positive microvesicles and SDF-1 in peripheral blood stem cell harvest

Bone Marrow Transplantation volume 41, pages 1071–1072 (2008)Cite this article

Cellular microvesicles (MVs) are released by various cell types such as circulating blood cells and cells from the vessel wall during cell activation by agonists and physical stress.1 MVs contribute to many pathophysiologic processes and are able to transfer various receptors. It is previously reported that MVs play a role in SCT.2, 3 In particular, Janowska-Wieczorek et al.2 reported that platelet-derived MVs bind to hematopoietic stem/progenitor cells and enhance their engraftment.

Stromal cell-derived factor-1 (SDF-1) is a CXC chemokine and a known chemotactic for lymphocytes and monocytes. SDF-1 is also the predominant chemokine that mobilizes stem/progenitor cells and has been shown to be upregulated in many damaged tissues as part of the injury response.4 In contrast, endothelial progenitor cells are a subtype of BM-derived progenitor cells expressing surface antigens of both hematopoietic stem cells and endothelial cells.5 Recently, Deregibus et al.6 found that endothelial progenitor cell-derived MVs were incorporated in endothelial cells by interaction with α4- and β1-integrins expressed on MV surfaces and suggested that endothelial progenitor cells may activate angiogenesis in endothelial cells by releasing MVs, which are able to trigger an angiogenic program. Some previous experimental studies have suggested that transplantation of stem and progenitor cells may have a beneficial effect on functional and structural recovery in several organs. However, there have been few investigations of the relationship between MVs and SDF-1 during PBSC harvest. We measured the levels of CD42b-negative/α4-integrin-positive MVs, SDF-1 and soluble CD40 ligand in plasma obtained from patients undergoing PBSC harvest.

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Authors and Affiliations

  1. Division of Hematology, Kishiwada City Hospital, Kishiwada, Osaka, Japan

    S Nomura & K Ishii

  2. Second Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan

    N Inami & Y Kimura

  3. Department of Internal Medicine, Matsushita Memorial Hospital, Moriguchi, Osaka, Japan

    N Uoshima

  4. Division of Hematology, Kinki University, Sayama, Osaka, Japan

    F Urase & Y Maeda

  5. Department of Internal Medicine, Keihanna Hospital, Hirakata, Osaka, Japan

    K Hayshi

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  1. S Nomura
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Correspondence to S Nomura.

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Nomura, S., Ishii, K., Inami, N. et al. α4-integrin-positive microvesicles and SDF-1 in peripheral blood stem cell harvest. Bone Marrow Transplant 41, 1071–1072 (2008). https://doi.org/10.1038/bmt.2008.38

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