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Cord Blood Stem Cells

Haematopoietic repopulating activity in human cord blood CD133+ quiescent cells

Bone Marrow Transplantation volume 43, pages 627–635 (2009)Cite this article

Abstract

We have demonstrated previously that cord blood CD133+ cells isolated in the G0 phase of the cell cycle are highly enriched for haematopoietic stem cell (HSC) activity, in contrast to CD133+G1 cells. Here, we have analysed the phenotype and functional properties of this population in more detail. Our data demonstrate that a large proportion of the CD133+G0 cells are CD38 negative (60.4%) and have high aldehyde dehydrogenase activity (75.1%) when compared with their CD133+G1 counterparts (13.5 and 4.1%, respectively). This suggests that stem cell activity resides in the CD133+G0 population. In long-term BM cultures, the CD133+G0 cells generate significantly more progenitors than the CD34+G0 population (P<0.001) throughout the culture period. Furthermore, a comparison of CD133+G0 versus CD133+G1 cells revealed that multilineage reconstitution was obtained only in non-obese diabetic/SCID animals receiving G0 cells. We conclude that CD133+ cells in the quiescent phase of the cell cycle have a phenotype consistent with HSCs and are highly enriched for repopulating activity when compared with their G1 counterparts. This cell population should prove useful for selection and manipulation in ex vivo expansion protocols.

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Acknowledgements

This work was supported by Yorkshire Cancer Research and Cancer Research UK, SJH European Commission Consert Grant 005242 and MPB EU Grant: EURO-POLICY-PID: Ref: SP23-CT-2005-006411. We are grateful to Liz Straczynski and Adam Davison for assistance with flow cytometry, our colleagues for cooperation with cord blood collection and Adrian Thrasher for support. We would like to thank Dr L Miall and staff of the Antenatal Unit at St James's University Hospital for their assistance with cord blood collection.

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Authors and Affiliations

  1. Department of Molecular Gastroenterology, Leeds Institute of Molecular Medicine, University of Leeds, Leeds, UK

    S A Boxall, G P Cook, Y M El-Sherbiny, J P Leek, A F Markham & E A de Wynter

  2. Haematopoietic Stem Cell Laboratory, Cancer Research UK, London Research Institute, London, UK

    D Pearce, D Bonnet & A F Markham

  3. Molecular Immunology Unit, Institute of Child Health, UCL, London, UK

    M P Blundell & S J Howe

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  1. S A Boxall
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Correspondence to E A de Wynter.

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Boxall, S., Cook, G., Pearce, D. et al. Haematopoietic repopulating activity in human cord blood CD133+ quiescent cells. Bone Marrow Transplant 43, 627–635 (2009). https://doi.org/10.1038/bmt.2008.368

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