Figure 3

From: Loss of Myosin Vb in colorectal cancer is a strong prognostic factor for disease recurrence

Figure 3

Frequently reduced expression of MYO5B is neither due to methylation nor mutations in CRC. (A) 5-aza-dC (DAC) treatment does not increase basal MYO5B expression in colon cancer cell lines. Data are representative of 2–4 independent experiments and presented as mean±s.d. (B) Methylation pattern analysed by mass array technology of eight CRC cell lines as well as eight tumour samples and four matching non-tumour counterparts. Each analysed cytosine is represented by a dot; cytosines with values below 5% were considered as non-methylated. (C) Analysis of MYO5B methylation in the TCGA data set covering methylation data on 295 tumour and 19 non-tumour samples. (D) MYO5B mutations in CRC patients. The protein domains and motifs are presented according to Qiu et al (2017), and the RAB8A- and RAB11A-binding sites according to Roland et al (2011). The dilute domain is also called globular tail domain. IQ=IQ motif, BD=binding domain.