Main

Aldehyde dehydrogenases (ALDHs) are enzymes that oxidise endogenous and exogenous aldehydes. To date, 19 ALDH isozymes have been identified in the human ALDH superfamily (Marchitti et al, 2008). Of these, ALDH1A1 has been shown to be associated with highly malignant types of solid tumours, such as cancers of the breast, lung, prostate, and ovary (Ginestier et al, 2007; Patel et al, 2008; Li et al, 2010; Meng et al, 2014), making this isozyme a potential predictive biomarker of these cancers. Expression of ALDHs in colorectal cancer (CRC) has also been investigated, but the evidence does not support ALDH1A1 as an effective biomarker for CRC (Kahlert et al, 2012; Zhou et al, 2015). We previously reported that another ALDH1 family member, ALDH1B1, might be a better CRC biomarker than ALDH1A1 (Chen et al, 2011); a role for ALDH1B1 in CRC tumourigenesis was subsequently identified (Singh et al, 2015).

The present study was designed to extend these previous studies by investigating which ALDH isozyme is most consistently expressed by or associated with CRC. First, we analysed mRNA levels of eight ALDH isozymes previously reported to be potential tumour markers (Marchitti et al, 2010; Marcato et al, 2011; Seidensaal et al, 2015) or to be carcinogenic (Nishiyama et al, 2015), including ALDH1A1 and ALDH1B1. Second, we investigated the possibility of post-translational or genetic modification of candidate ALDHs by analysing mRNA and protein expression, enzymatic activity levels, and cDNA sequences. Finally, the potential of candidate ALDHs for detecting CRC was tested using tissue microarray, which included normal/CRC paired samples.

Materials and methods

Explants and cell lines

Patient-derived CRC tissue samples were collected from consenting patients between 2007 and 2011 at the University of Colorado Hospital in accordance with protocols approved by the Colorado Multiple Institutional Review Board. They were obtained from 26 patients aged 32–73 (mean, 54.5 years) who had primary or metastatic CRC of tumour–node–metastasis (TNM) stages II–IV.

Immortalised human CRC cell lines, that is, BE, Caco-2, COLO320 DM, HCT116, HT29, and SW480 cells at passage 4 (p4), p26, p2, p4, p4, and p4 (respectively), were kindly provided by Dr David Ross (University of Colorado School of Pharmacy). All cell lines were cultured in plastic flasks or Petri dishes in RPMI 1640 medium supplemented with 10% foetal bovine serum, 100 U ml−1 penicillin, 100 μg ml−1 streptomycin, and 0.25 μg ml−1 amphotericin B (Sigma-Aldrich, St Louis, MO, USA). Cells were maintained at 37 °C in an environment of 5% CO2 in air. Media and supplement exchanges were performed twice weekly.

Messenger RNA sequencing

Total RNA from each explant was extracted using the RNAeasy kit (Qiagen, Germantown, MD, USA). Total RNA libraries were constructed according to the Illumina TruSeq RNA Sample Preparation v2 Guide (Illumina Inc., San Diego, CA, USA). Complementary DNA library integrity was determined on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The Illumina cBot system was used for cluster generation, and sequencing was performed on the Illumina HiSeq 2000 (Illumina). A de-multiplexing step was carried out before the alignment step for multiplexed lanes/samples. Mapping against the human genome (Trapnell et al, 2009) was performed with TopHat (version 1.3.2). Gene expression was estimated as the sum of the normalised fragment counts (fragment number/kilobase of exon/million mapped fragments (FPKM)) of multiple transcripts that represent the same gene (Garber et al, 2011). The fragment count was normalised to the exon length and total fragment amount to achieve a meaningful comparison between samples and genes.

Quantitative PCR

Total RNA from CRC cell lines was extracted as described above. The cDNA was then synthesised using a SuperScript I First-Strand Synthesis System (Invitrogen, Frederick, MD, USA). Ribonucleic acid from sorted cell populations was extracted using TRIzol (Invitrogen) in a different laboratory, transported at −20 °C, and stored at −80 °C until cDNA synthesis was conducted. Messenger RNA levels were analysed with respective primers (Supplementary Table S1) and the SYBR Green PCR Master Mix using ABI PRISM 7000 (Applied Biosystems, Foster City, CA, USA). Individual ALDH mRNA copy numbers were quantified using a standard curve generated from respective cDNA constructs of the gene.

Protein assays

Cells were scraped from culture dishes, resuspended in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% sodium dodecyl sulphate) containing proteinase inhibitors at the concentration recommended by the manufacturer (Promega, Madison, WI, USA), and sonicated (10 s, three times) to obtain homogenates. Protein concentrations in the homogenate were assessed using a Bio-Rad Protein Assay Kit (Bio-Rad, Hercules, CA, USA). Protein separation and western blotting were performed using protocols and antibodies previously described (Chen et al, 2011). β-actin was used as a loading control. Quantitative analysis of the signal intensity of western blot protein bands was performed using ImageJ (National Institutes of Health, Bethesda, MD, USA).

Enzyme activity assay

Freshly collected lysates of CRC cell lines obtained by scraping were washed with phosphate-buffered saline (4 °C, pH 7) and homogenised in 0.1 M potassium phosphate buffer (pH 7.5) containing 1 mM EDTA and 1 μ M 2-mercaptoethanol. Aldehyde dehydrogenase enzymatic activity of whole homogenates was determined by monitoring the formation of NADH (when acetaldehyde and propionaldehyde were used as substrates) and NADPH (when benzaldehyde was used as substrate) at 340 nm during the oxidation of aldehyde substrates for 5 min at room temperature using a spectrophotometer (Beckman DU-640) (molar extinction coefficient: 6.22 mM−1 cm−1). The reaction was initiated by adding 0.1 ml of substrate (1 mM acetaldehyde, 100 μ M propionaldehyde, or 500 μ M benzaldehyde) to 0.9 ml of reaction mixture containing 0.1 M sodium pyrophosphate (pH 8.0), 20 mM NAD or 50 mM NADP, 20 mM pyrazole, 1 mM 2-mercaptoethanol, and 50 μl of cell homogenate (5–8 mg protein ml−1).

Flow cytometry and cell sorting

After gentle cellular detachment from the culture plate using trypsin (0.04%)/EDTA (0.03%) solution, cells were resuspended and washed in phosphate-buffered saline, and pelleted by centrifugation. CD44 staining was performed using a monoclonal antibody and reagent from BD Biosciences (San Jose, CA, USA). Aldehyde dehydrogenase detection was performed using the Aldefluor assay (StemCell Technologies, Vancouver, BC, Canada) as follows: a total of 1 × 106 cells ml−1 was resuspended in Aldefluor buffer and incubated at 37 °C for 30 min with 2.5 μl ml−1 activated Aldefluor reagent, which includes a fluorescent-labelled substrate of ALDHs. Baseline fluorescence was established using control cells in the presence of 5 μl ml−1 of 1.5 mM dimethylaminobenzaldehyde (DEAB), an ALDH inhibitor. Cell staining and analytical procedures were performed either manually or using high-content semi-automated flow cytometry as described previously (Gasparetto et al, 2004). Flow cytometric analysis and cell sorting were performed on a FACSAria device equipped with 405 nm violet, 488 nm argon, and 633 nm HeNe lasers (Becton Dickinson, San Jose, CA, USA). Data were analysed using FlowJo software (TreeStar, Palo Alto, CA, USA).

Tissue microarray

A tissue microarray (TMA) was constructed at the University of Colorado Pathology Department from previously formalin-fixed paraffin-embedded specimens (22 normal and 32 tumour tissues, including 22 matched pairs of normal and tumour tissue from the same patient) in accordance with protocols approved by the Colorado Multiple Institutional Review Board. A hollow needle was used to remove representative 0.6-mm diameter tissue cores (in duplicate for each specimen) from different locations while avoiding necrotic tissue. The TMA was designed with control normal tissue samples that included: appendix, bladder, breast, colon, liver, ovary, pancreas, and spleen. The 0.6 mm cores were cut into 5 μm sections and adhered to glass slides.

Slides were deparaffinised with xylene and rehydrated with ethanol. Antigen retrieval was performed using citrate buffer (pH 6) at a temperature of 97 °C for 20 min. After blocking of endogenous peroxidase with methanol and hydrogen peroxide, slides were preincubated with 0.3% bovine serum albumin in 0.1 M Tris-buffered saline for 30 min at room temperature. Slides were then incubated with cytokeratin and the primary antibody for ALDH1A1, ALDH1B1, and ALDH2 at previously optimised staining conditions. Mouse/rabbit EnVision reagent (Agilent Technologies, Santa Clara, CA, USA) and Alexa 546-conjugated goat anti-rabbit/mouse secondary antibody (Molecular Probes, Eugene, OR, USA; 1 : 100 dilution) were used as secondary antibodies that were visualised using Cy5-tyramide (PerkinElmer, Waltham, MA, USA). 4′6-diamidino-2-phenylindole (DAPI) staining was used to identify tissue nuclei.

Automated quantitative analysis

Automated quantitative analysis (AQUA) was performed to allow the objective and accurate measurement of protein expression within defined tumour areas and subcellular compartments as described previously (Camp et al, 2002; McCabe et al, 2005). Briefly, after immunofluorescent staining of the TMA, a series of monochromatic, high-resolution images were captured using an Olympus AX-51 epi-fluorescent microscope (Olympus, Tokyo, Japan). For each histospot represented on a TMA, images for three different channels were obtained to visualise nuclei, cytokeratin, and ALDHs using DAPI, Alexa 546, and Cy5-tyramide, respectively. To set the area for quantification, the cytokeratin signal was binarised. The pixel intensity of antibody-labelled ALDHs was measured within these compartments and divided by the compartment area, resulting in a continuous scoring system, which was directly proportional to the concentration of ALDHs.

Statistical analyses

Statistical analyses were performed using StatView software for Windows Version 5.0 (SAS Institute Inc., Cary, NC, USA). The Mann–Whitney U-test was used to compare two groups of mRNA levels of human CRC explants. Regression analyses were conducted to detect significant associations among mRNA and protein expression and enzymatic activity levels across all cell lines studied. Paired or unpaired t-tests (as appropriate) were performed to detect differences in immunohistochemical signal intensity between normal tissue and CRC tissue. A value of P<0.05 was considered to be significant.

Results

ALDH2 and ALDH1B1 mRNA is highly expressed in human CRC tissue and cell lines

Of the eight ALDHs investigated, the highest mRNA expression was observed for ALDH1A1 in a human tissue (329 FPKM); however, it showed great expression variability among the tumour samples, with the lowest level <1 FPKM. Expression of ALDH1B1, ALDH2, ALDH3A2, and ALDH3B1 was >1 FPKM in all tumour tissue samples (each minimum value was 3, 36, 5, 2, respectively), while ALDH1A1, ALDH1A2, ALDH1A3, and ALDH3A1 showed <1 FPKM in some or all samples. Cancer stage (TNM II/III vs TNM IV) had no impact (P>0.05) on mRNA expression levels of the ALDH isozymes examined (Figure 1A). Similarly, no differences (P>0.05) were found in mRNA expression levels between primary CRC and metastatic CRC samples (Figure 1B).

Figure 1
figure 1

ALDH mRNA levels in human CRC tissue samples by RNA sequencing. (A) Messenger RNA expression levels according to TNM classification. TNM stage II or III (white boxes, n=9) and IV (grey boxes, n=17). (B) Messenger RNA expression levels according to primary tumours (white boxes, n=18) or metastases (grey boxes, n=8). Bottom and top of each box represents the first and third quartiles, respectively. Horizontal line in the box indicates the median. The ends of the whiskers are maximum and minimum values.

As seen for the explants, ALDH1B1 and ALDH2 mRNA was regularly expressed in the six cell lines (Figure 2A). ALDH1A1 mRNA expression levels varied widely among the cell lines. Other ALDHs showed different patterns of expression between explants and cell lines and were not consistently detected in all samples. For example, ALDH3A2 and ALDH3B1 mRNA expression was detectable in tumour tissues but undetectable in almost all cell lines. ALDH1A2 and ALDH1A3 expression was low and undetectable in many tumour tissue samples (median, <1 FPKM) but showed high expression in some cell lines.

Figure 2
figure 2

Messenger RNA and protein expression and enzymatic activity of ALDH isozymes in human CRC cell lines. (A) Messenger RNA expression levels were measured by real-time PCR. Data are presented as the mean of three replicates. (B) Western blot analysis of ALDH1A1, ALDH1B1, ALDH2, and β-actin. Ten μg of protein was applied to each lane. (C) ALDH enzymatic activity. Substrates tested include acetaldehyde, proprionaldehyde, and benzaldehyde. Data are presented as the mean and associated s.e.m. (n=3).

Relationship between mRNA, protein expression, and enzyme activity levels in human CRC cell lines

Aldehyde dehydrogenase 1A1 protein expression was not detected in BE, HCT116, or SW480 cells. ALDH1B1 and ALDH2 were detected in all of the cell lines (Figure 2B). Regression analysis of protein and mRNA levels revealed a significant relationship for ALDH1A1 (r=0.88, P=0.02) and ALDH2 (r=0.82, P=0.05) in all cell lines. Aldehyde dehydrogenase 1B1 showed a weaker correlation between its mRNA and protein expression levels (r=0.65, P=0.17).

The ALDH enzymatic activities were assessed in the cell lines using three major substrates: acetaldehyde and propionaldehyde (being reflective of ALDH1A1, ALDH1B1, and ALDH2 activity) (Lassen et al, 2005; Manzer et al, 2003; Stagos et al, 2010), and benzaldehyde (indicative of ALDH3 activity) (Pappa et al, 2003). Acetaldehyde metabolising activities correlated with mRNA levels of ALDH1B1 (r=0.92, P=0.04) and ALDH2 (r=0.98, P<0.01). Similarly, metabolism of propionaldehyde correlated with ALDH2 mRNA levels (r=0.93, P=0.02), but not with ALDH1B1 mRNA levels (r=0.60, P=0.29). ALDH1A1 mRNA levels showed no significant correlation with any examined enzymatic activities, while ALDH3A1 mRNA levels correlated with enzymatic activity for benzaldehyde (r=0.95, P=0.01). The relationship between protein levels and enzymatic activity for acetaldehyde was almost significant for ALDH2 (r=0.88, P=0.05) and ALDH1B1 (r=0.86, P=0.06), no correlation was observed for ALDH1A1 (r=0.80, P=0.10). In the case of propionaldehyde enzymatic activity, ALDH1A1 and ALDH2 were significantly correlated (r=0.97, P<0.01 or r=0.97, P<0.01, respectively), while ALDH1B1 showed a weaker correlation between protein and enzymatic activity levels (r=0.78, P=0.12).

Taken together, mRNA and protein expression and enzymatic activities were correlated for ALDH2. Although ALDH1A1 and ALDH1B1 showed the same tendency, the level of correlation was much weaker than that for ALDH2. To examine the possibility that ALDH1B1 was functionally modified by genetic alterations in CRC, we examined the entire ALDH1B1 cDNA sequence of the six CRC cell lines but found no differences among any of the cells (Supplementary Table S2).

ALDH isozyme mRNA expression in a highly tumourigenic cell population

In a xenograft model, cells exhibiting high ALDH enzymatic activity (ALDH+) and expressing CD44 (CD44+) or CD133 (CD133+) were shown to have a stronger potential to initiate and expand tumours than cells that had high ALDH enzymatic activity alone (Huang et al, 2009). In the present study, mRNA expression for all ALDH isozymes was higher in ALDH+CD44+ cells than in the total ALDH+ cell population. However, only ALDH1B1 and ALDH2 mRNA was expressed in all cell lines (Figure 3). The expression of ALDH3A1, ALDH3A2, and ALDH3B1 mRNA was not analysed because levels in the cell populations were low, that is, less than 10 copies ng−1 RNA.

Figure 3
figure 3

ALDH+CD44+ cells showed high expression of ALDH1B1 and ALDH2. (A) Schematic of Aldefluor system. The ALDH+ population was selected by its ability to metabolise aminoacetaldehyde. Fluorescent dye-modified aminoacetaldehyde (BAAA) was applied to cells in the presence or absence of ALDH inhibitor (DEAB). Cells that fluoresced in the presence of DEAB were excluded (background). (BD) Selection of subpopulations. Representative two-parameter scatter plots of flow cytometry are shown for COLO320 DM cells. Blue box shows the selected population. (B) Selection of ALDH+ subpopulation. Right panel shows background fluorescence, that is, cells fluorescing in the presence of DEAB. (C and D) Selection of ALDH+CD44+ subpopulation. The ALDH+CD44+ subpopulation was selected from the whole population, that is, no background subtraction step was included (C). ALDH+CD44+ cells were post-tested with ALDH-SSC two-parameter histograms (confirmation of background exclusion) (D). (E) Aldehyde dehydrogenase isozyme mRNA levels in the subpopulations. Messenger RNA expression levels were measured in ALDH+ and ALDH+CD44+ subpopulations separated from the whole population on separate days. Data are the mean of three replicates. A full colour version of this figure is available at the British Journal of Cancer journal online.

AQUA analysis of ALDH isozymes in CRC

To determine the utility of using ALDHs (e.g., ALDH1B1 and ALDH2), for CRC detection, the TMA was tested using the AQUA system (Figure 4). Among the 22 matched specimens (i.e., normal and tumour tissue from the same donor), nine pairs for ALDH1B1 and 10 pairs for ALDH1A1 and ALDH2 were successfully quantified with AQUA. Unexpectedly, the AQUA score for ALDH1A1 was significantly lower in CRC than in normal tissue (P=0.012), while that for ALDH1B1 was higher in CRC than in normal tissue (P=0.048). No difference was detected for ALDH2 (P=0.122). The ratio of ALDH1B1 to ALDH1A1 or ALDH1B1 to ALDH2 was found to be powerful in detecting differences between normal and CRC tissue (P=0.018 and 0.034, respectively) (Figure 4C), which was reproduced in an unmatched comparison with whole samples that were successfully quantified in normal tissue (n=13) and CRC tissue (n=24) (P<0.0001 for both ratios) (Figure 4D).

Figure 4
figure 4

Fluorescent multiplexed analysis of ALDHs. (A) Representative histological images (magnification= × 400) of normal and CRC tissue. Blue: nuclei, green: cytokeratin, red: ALDH. (B) Automated quantitative analysis scores for ALDH1A1, ALDH1B1, and ALDH2 in matched pairs of normal and CRC tissue obtained from the same donors (*P<0.05, paired t-test). (C) Automated quantitative analysis score ratio of ALDH1B1 to ALDH1A1 or ALDH1B1 to ALDH2 in normal and CRC matched pairs of tissue (*P<0.05, paired t-test). (D) Ratio of ALDH1B1 to ALDH1A1 or ALDH1B1 to ALDH2 in normal and CRC tissue obtained from 24 donors (***P<0.0001, unpaired t-test). Data represent the mean±s.e.m. from N samples.

Discussion

ALDH1B1 and ALDH2 mRNA expression in human CRC tissue samples and cells was found to be consistently high. This was not the case for other ALDH isozymes, which were undetectable or low in some or all samples. Western blot and ALDH enzymatic activity analysis of CRC cell lines revealed significant correlations among mRNA and protein expression and enzymatic activity for ALDH2, and weaker correlations for ALDH1B1. Based on this observation, cDNA sequences were analysed in all of the cell lines to determine whether ALDH1B1 was functionally modified by genetic alterations in CRC; however, no mutation was found. ALDH1B1 and ALDH2 mRNA was highly expressed in ALDH+CD44+ cells (a proposed highly tumourigenic stem cell population (Huang et al, 2009)) in all human cell lines examined. Finally, paired normal and CRC tissues from the same donors were compared using the AQUA system, which showed that ALDH1B1, but not ALDH2, is expressed at higher levels in CRC than in normal tissue. Notably, the ratio of ALDH1B1 to ALDH1A1 or ALDH2 showed a strong ability to distinguish CRC tissue from normal tissue.

ALDH1B1 is a promising CRC marker

Among the eight isozymes tested, only the expression of ALDH1B1 was consistently found to correlate with the presence of CRC in cells and tissue samples. Furthermore, the ratios of ALDH1B1/ALDH1A1 and ALDH1B1/ALDH2 were found to be powerful CRC detectors. In support of this proposal, a previous study investigating potential CRC markers (i.e., CD29, CD44, ALDH1A1, ALDH1B1, EpCam, and CD166) showed that only ALDH1B1 could distinguish CRC from normal tissue (Langan et al, 2012). ALDH2 showed high expression in the human CRC cell lines but was less able to distinguish CRC tissue from normal tissue in human CRC explants. This may relate to ALDH2 being expressed in various normal tissues (Stewart et al, 1996; Stagos et al, 2010; Chiang et al, 2012). Given the inconsistent or low mRNA profiles of other ALDHs, including ALDH1A1, it may be speculated that their contribution to CRC is relatively small. The present results do not support a role for ALDH1A1 in CRC detection because its expression in human cell lines was inconsistent and its AQUA scores decreased in CRC tissues. As such, we suggest measurement of ALDH1B1 expression using the AQUA system could be a useful marker for the detection of CRC tissue.

ALDH1B1 is a functionally important cancer-related factor

We recently reported that ALDH1B1-silenced SW480 cells showed inhibited proliferation in a spheroid assay and xenograft model (Singh et al, 2015). The effect of ALDH1B1 in tumourigenesis appears to involve modulation of the Wnt-, Notch-, and PI3K/Akt signalling pathways (Singh et al, 2015). Our current finding that a supposedly highly tumourigenic CRC subpopulation shows high expression of ALDH1B1 mRNA lends further support to this hypothesis.

Based on its possible ability to enzymatically convert retinal to retinoic acid, ALDH1B1 is presumed to promote differentiation of stem cells (Chute et al, 2006; Jackson et al, 2015). Indeed, a previous study reported that ALDH1B1 protein expression in human CRC was increased in line with the extent of differentiation (Langan et al, 2012). Given that differentiation reduces the self-renewing capacity of cells (Todaro et al, 2010), ALDH1B1 would be predicted to decrease the degree of malignancy. However, such an anti-cancer action would be contrary to the tumourigenic role of ALDH1B1 proposed herein. Recently, we found that the PI3K/Akt pathway could be accelerated by retinoic acid (Singh et al, 2015). This pathway regulates cell growth under normal conditions; however, in CRC, it becomes hyper-activated by the aberration of associated genes, resulting in the promotion of malignant growth (Danielsen et al, 2015). Retinoic acid may therefore have two contradictory roles in CRC: one in the differentiation of stem cells that reduces the potential of malignancy, and another in promoting tumour growth through a hyper-activated PI3K/Akt pathway.

Future challenges in the application of ALDH1B1 as a CRC marker

For the practical application of a proposed marker, a cutoff value to distinguish CRC from normal colorectal tissue needs to be established; this will require evaluation in a larger number of specimens.

The possibility of using ALDH1B1 as a predictive biomarker for CRC should be examined in future studies. A pilot study by Langan and colleagues (2012) suggested an association between ALDH1B1 expression and CRC differentiation, but it failed to detect an association between ALDH1B1 disease stage; ALDH1A1 was shown to be linked with increased disease stage. The present study also failed to show an association between ALDH1B1 and cancer stage (TNM II/III vs TNM IV).

In conclusion, our present work suggests that cellular expression of ALDH1B1 (as determined by immunohistology) or the ratio of ALDH1B1 to ALDH1A1 (or ALDH2) in tissue samples could be used as a novel biomarker for identifying CRC.