Workflow for the in silico analysis of miR-874 -regulated pathways. miR-control transfectants that produced raw signal values of <1000 were excluded before comparisons were made. Downregulated genes in miR-874 transfectants with <−0.5 (log2 ratio) downregulation compared with the control transfectants were chosen from the results of microarray analysis (GSE37119). A total of 1071 and 591 genes were identified as genes downregulated by miR-874 in SAS and FaDu cells, respectively. The gene sets were then analysed and categorised with the KEGG Pathway Database using the GENECODIS 3.0 program. In all, 59 and 13 significantly enriched signalling pathways were derived from the gene sets in SAS and FaDu cells, respectively. In total, 13 and 8 genes were included in the ‘cell cycle’ pathway in SAS and FaDu cells, respectively.