Abstract
Background:
Enzastaurin, an oral serine–threonine kinase inhibitor, was initially developed as an ATP-competitive selective inhibitor against protein kinase Cβ. However, the mechanism by which enzastaurin contributes to tumourigenesis remains unclear.
Methods:
We analysed the anti-tumour effects of enzastaurin in 22 lung cancer cell lines to ascertain the potential for enzastaurin-based treatment of lung cancer. To identify molecules or signalling pathways associated with this sensitivity, we conducted a gene, receptor tyrosine kinases phosphorylation and microRNA expression profiling study on the same set of cell lines.
Results:
We identified eight genes by pathway analysis of molecules having gene-drug sensitivity correlation, and used them to build a support vector machine algorithm model by which sensitive cell lines were distinguished from resistant cell lines. Pathway analysis revealed that the JAK/STAT signalling pathway was one of the main ones involved in sensitivity to enzastaurin. Overexpression of JAK1 was observed in the sensitive cells by western blotting. Simultaneous administration of enzastaurin and JAK inhibitor inhibited enzastaurin-induced cell growth-inhibitory effect. Furthermore, lentiviral-mediated JAK1-overexpressing cells were more sensitive to enzastaurin than control cells.
Conclusion:
Our results suggested that the JAK1 pathway may be used as a single predictive biomarker for enzastaurin treatment. The anti-tumour effect of enzastaurin should be evaluated in lung cancer with overexpressed JAK pathway molecules.
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Main
Non-small-cell lung cancer (NSCLC) patients are usually diagnosed with advanced disease, and their prognosis remains poor despite improvements in chemotherapies (Mountain, 1997; Schiller et al, 2002; Ohe et al, 2007; Jemal et al, 2009). Recently, molecular-targeted therapies have been developed for NSCLC treatment. For example, NSCLC patients with epidermal growth factor receptor (EGFR) mutations have shown a dramatic response to EGFR inhibitors such as gefitinib and erlotinib (Mok et al, 2009; Maemondo et al, 2010). However, there remain many other molecular abnormalities in lung cancer that are as yet unexplored (Salgia and Skarin, 1998).
The protein kinase C (PKC) family of serine–threonine protein kinases has been implicated in several important cellular functions including proliferation, motility, invasion and apoptosis (Livneh and Fishman, 1997). Among the PKC isoforms, PKCβ is known to be an important mediator of vascular endothelial growth factor (VEGF) (Xia et al, 1996; Yoshiji et al, 1999), the most potent angiogenic factor found in various tumours. Increased invasion and proliferation in tumours have also been associated with PKCβ (Zhang et al, 2004). Overexpression and increased activity of PKCβ have been implicated in transformation and tumourigenesis in lung cancer (Barr et al, 1997; Lahn et al, 2006). In several human cancers, PKCβ expression is linked to poor prognosis, most notably in B-cell lymphoma (Shipp et al, 2002; Li et al, 2007). Biochemical analysis demonstrated that PKCβ could target the phoshatidylinositol 3-kinase (PI3K)/AKT pathway and other signal transduction pathways (Graff et al, 2005; Rascoe et al, 2005). However, the mechanism by which PKCβ contributes to tumourigenesis remains unclear.
The PKCβ inhibitor enzastaurin, an oral serine–threonine kinase inhibitor, was initially developed as an ATP-competitive selective inhibitor against PKCβ (Faul et al, 2003). Enzastaurin is now being evaluated in several phase II studies across a variety of more common tumour types including: breast, ovarian colon and prostate cancers (Mina et al, 2009; Vergote et al, 2009; Dreicer et al, 2010; Glimelius et al, 2010). It has also been evaluated as second- or third-line therapy for NSCLC in a phase II study (Oh et al, 2008; Chiappori et al, 2010). In vitro, sequence-dependent, synergistic anti-proliferative and proapoptotic effects of the combination of cytotoxic drugs and enzastaurin have been found in NSCLC cells (Rademaker-Lakhai et al, 2007; Morgillo et al, 2008; Tekle et al, 2008). These studies suggest that enzastaurin may have an activity against lung cancer.
In this study, we analysed the anti-tumour effects of enzastaurin in a panel of 22 lung cancer cell lines to ascertain the potential for enzastaurin-based treatment of lung cancer. We also conducted gene, receptor tyrosine kinases (RTKs) phosphorylation and microRNA (miRNA) profiling on the same set of cell lines to identify the molecules associated with sensitivity of lung cancer to enzastaurin treatment. The correlation between the cytotoxic activity of enzastaurin and the corresponding gene, RTKs phosphorylation and miRNA expression patterns has been examined to clarify the responsible mechanisms of the signalling pathway involved in the response of lung cancers to enzastaurin treatment.
Materials and methods
Cell lines
We used 22 lung cancer cell lines: A549, PC3, PC7, PC9, PC14, LC2/ad, ABC-1, RERF-LC-KJ, RERF-LC-MS, RERF-LC-AI adenocarcinoma (AC) cell lines and PC1, PC10, LK2, SQ5, QG56, EBC-1, LC1/sq squamous-cell carcinoma (SCC) cell lines and NCI-H69, NCI-N231, Lu135, SBC3, MS-1 small-cell lung carcinoma (SCLC) cell lines for this study. In addition, five cell lines comprising H1650, H1975, LC-1F, RERF-LC-OK and VMRC-LCD, were used as the test set for a validation study. A549, NCI-H69, NCI-N231, H1650 and H1975 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA); RERF-LC-KJ, RERF-LC-AI, RERF-LC-OK, LC2-ad, SQ5, LC2/Ad, LC1/Sq, LC-1F and MS-1 were obtained from the RIKEN Cell Bank (Ibaraki, Japan) and PC1, PC3, PC7, PC9, PC10 and PC14 were obtained from Immuno-Biological Laboratories (Gunma, Japan); RERF-LC-MS, ABC-1, EBC-1, LK2, QG56 and VMRC-LCD were purchased from Health Science Research Resources Bank (Osaka, Japan). Lung cancer cell lines were maintained in RPMI 1640 medium (GIBCO, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum.
Drugs and growth-inhibition assay
Enzastaurin was kindly provided by Ely Lilly. Growth inhibition was assessed by MTS assay to examine the effect of enzastaurin on lung cancer cell lines. Cell suspensions (5000 cells per well) were seeded into 96-well plates and increasing concentrations of enzastaurin (0, 0.01, 0.1, 1.0, 10 and 100 μ M) were added. After incubation for 72 h at 37 °C, MTS was added to each well and incubated for 2 h at 37 °C, after which absorbance was measured using a microplate reader with a test wavelength of 450 nm. The IC50 value was defined as the concentration needed for 50% reduction of the growth by treatment with enzastaurin.
JAK inhibitor (JAK inhibitor I, Cat. No 420099) was purchased from Calbiochem (San Diego, CA, USA). A549 and RERF-LC-KJ cells (5000 cells per well) were seeded into 96-well plates. After 24 h, the cells were incubated for 72 h in the various concentrations of enzastaurin (0, 0.01. 0.1, 1.0, 10 and 100 μ M), with or without low-dose (1 μ M) JAK inhibitor.
RNA isolation, cDNA array, RTKs phosphorylation antibody array and miRNA array
Total RNA was isolated from lung cancer cell lines with the use of TRIzol reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions. High-density oligonucleotide array analysis was carried out using Affymetrix HG-U133A (22 282 probe sets) expression array, as previously described (Gemma et al, 2006). Scanning was performed with the GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA, USA), and GeneChip analysis was based on the Affymetrix GeneChip Manual with GeneChip Operating Software version 1.0 (Affymetrix), and Microarray Database software. We also performed human RTKs phosphorylation antibody array, including 71 antibodies (RayBiotech, Inc., Norcross, GA, USA). MicroRNA expression profiles were analysed by TaqMan MicroRNA Array set version 2.0 containing 667 miRNAs and validated by TaqMan MicroRNA assay (Applied Biosystems, Foster City, CA, USA).
Western blot analysis
Cells were lysed in buffer containing 50 mM Tris–HCl, pH 7.6, 150 mM NaCl, 0.1% sodium dodecyl sulphate, 1% Nonidet P-40 and 0.5% sodium deoxycholate. The lysates were kept on ice for 30 min, and then centrifuged at 13 000 g for 30 min. The supernatant was collected and 10 μg of protein were separated by gel electrophoresis on 10% gels, transferred to nitrocellulose membranes and detected by immunoblotting using a chemiluminescence system (GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA). The antibodies detecting JAK1, STAT3, phospho-STAT3 (p-STAT) and β-actin were purchased from Cell Signaling Technology (Beverley, MA, USA).
Lentiviral-mediated JAK1-overexpressing cells
Expression plasmid vector pEZ-Lv151 was used for lentiviral vector production (GeneCopoeia, Rockville, MD, USA). The coding sequence of human JAK1 or enhanced green fluorescent protein (EGFP) was inserted under the transcriptional control of the CMV promoter in pEZ-Lv151. The human JAK1 lentiviral expression plasmid (Ex-T8644-Lv151) or EGFP plasmid (Ex-EGFP-Lv151) was cotransfected into 293Ta cells with the Lenti-Pac HIV Packaging Mix (GeneCopoeia). Lentivirus-containing supernatants were harvested 48 h after transfection. The lentivirus particles were purified and stored at −80 °C in aliquots until use.
To establish stable JAK1-overexpressing cell lines, A549 cells were transduced with serial dilutions of lentiviral supernatant in the presence of 5 μg ml–1 polybrene and selected by 0.8 ng ml–1 geniticine. After antibiotic selection for 3 weeks, stable overexpressing JAK1 cells (LV-JAK1 A549 cells) were obtained.
Statistical analyses
Data analysis for the correlation coefficients that revealed the correlation between the drug activity patterns and the gene expression patterns was principally done by a modified National Cancer Institute programme (Miyanaga et al, 2008). We used pathway analysis to provide a viewpoint of the biological function of genes within the proposed classifier. Pathway analysis was done using the Pathway Architect software (Stratagene, La Jolla, CA, USA). The pathways showing the relationships among the genes on the list were drawn by selecting all molecules on the pathway edit window. All relationships among the molecules were retrieved from the database, with this information being derived from PubMed abstracts by natural language processing technology. The function was done by selecting the data of maximum reliability (MAX) by choosing all modes of interactions including ‘Promoter Binding’, ‘Regulation’, ‘Protein Modification’ and ‘Expression’ and by taking the relationships supported by three or more consistent data sources. Next, we picked out the incorporated genes from the imported gene list used at the onset of the pathway analysis, except the subunits of the target gene. Thus, a list of the genes associated with drug response was established with respect to not only gene expression profile data but also the biological functions of altered/associated genes. Data from the listed genes were used to build a support vector machine (SVM) model with ArrayAssist software (Stratagene) to predict the drug response (IC50). The SVM algorithm model with Gaussian kernels was used to distinguish sensitive cells from resistant cells, using biomarkers identified by the gene expression-enzastaurin drug sensitivity correlation and pathway analysis. The classification ability of the genes was evaluated using leave-one-out cross-validation.
Results
Effect of enzastaurin on the growth of lung cancer cells
Growth-inhibitory effects of enzastaurin on lung cancer cell lines were assessed by MTS assay.
Figure 1 shows the sensitivity to enzastaurin among the 22 lung cancer cells. Based on the IC50, the 22 cell lines were classified into two groups, namely: enzastaurin sensitive and enzastaurin resistant. Five cell lines (A549, RERF-LC-KJ, LC2/ad, RERF-LC-MS and SQ5) were sensitive (IC50 of ⩽10 μ M), and the remaining 17 cell lines were resistant to enzastaurin (IC50 of >50 μ M). The five cell lines sensitive to enzastaurin consisted of four AC (4/10, 40%) and one SCC (1/7, 14%) cell line; no SCLC (0/5) cell lines were enzastaurin sensitive. These results suggest that enzastaurin has anti-tumour activity against NSCLC.
Gene expression-drug sensitivity correlation
We have previously performed gene expression profile analysis of the same set of 22 lung cell lines by Affymetrix GeneChip (Gemma et al, 2006). First, we used the MTS results for enzastaurin for the development of a molecular model of sensitivity to enzastaurin. Twenty-three genes were significantly correlated with sensitivity to enzastaurin (correlation coefficients of >0.65). Next, pathway analysis was performed using the 23 genes to provide a viewpoint of the biological function of the genes, as previously described (Miyanaga et al, 2008). Pathway analysis removed the incorporated genes out of the imported 23 genes. Sixteen genes, associated with sensitivity to enzastaurin, were identified based on the biological functions of altered/associated genes (Table 1; Figure 2A). Pathway analysis revealed that JAK1 was the final target gene for the sensitivity to enzastaurin in lung cancer cells (Figure 2A). We next identified the optimal number of genes whose expression could accurately distinguish the sensitive cells from the resistant ones. Analysis of variance (ANOVA) was done to remove the genes with variance. The top eight genes (DUSP1, ILF3, LITAF, JAK1, COPS7B, RAD23A, TNFAIP1 and MIRN21/TMEM49) according to the ANOVA were subsequently found to be the minimum number necessary for prediction of drug response (Figure 2B; Table 1). We used the eight most strongly correlated genes to build an SVM algorithm model by which the five sensitive cells were distinguished from the 17 resistant cells. Overall, the SVM classification based on the above-mentioned eight genes, correctly classified the sensitivity to enzastaurin of all of the 22 cells (data not shown). Next, we examined the robustness of the eight-gene predictor, for classifying cells into the enzastaurin-sensitive group, in an independent set of NSCLC cells, and found that the eight-gene predictor correctly classified all five resistant cells (Table 2). Thus, we had ultimately identified an eight-gene signature that was validated for its ability to predict the sensitivity to enzastaurin in an independent set of lung cancer cells.
RTKs phosphorylation and miRNA expression-drug sensitivity correlation
Pathway analysis revealed that JAK1 was an important gene for the sensitivity to enzastaurin in lung cancer cells. JAK1 and its downstream STAT3 gene expression levels of sensitive cells were significantly higher than those of resistant cells (Figures 3A and B). To further clarify the signalling mechanism correlated with the sensitivity to enzastaurin, we also examined RTKs phosphorylation expression profiles of the same set of 22 lung cancer cells. The top 10 RTKs phosphorylation associated with enzastaurin sensitivity are shown in Table 3 (correlation coefficients of >0.50). Pathway analysis using the 23 genes and 10 RTKs phosphorylation associated with sensitivity to enzastaurin also revealed that JAK/STAT signal pathway was mainly involved in the drug response (data not shown). Among the 10 RTKs phosphorylation, the expression of two RTKs mainly associated with angiogenesis and lymphangiogenesis (VEGFR2 and VEGFR3) was significantly elevated in sensitive cells compared with in resistant cells (Figures 3C and D).
In order to investigate post-transcriptional regulation, miRNA microarray analysis of the 22 cells was also performed. We identified 13 miRNAs correlated with enzastaurin sensitivity (correlation coefficients of >0.50) (Table 3). Interestingly, MIRN21/TMEM49, a host gene of miR-21, was included among the eight genes associated with enzastaurin sensitivity, and was expressed at significantly higher levels in sensitive cells compared with in resistant cells (Figure 3E). In addition, a correlation between miR-21 and enzastaurin sensitivity was found in miRNA array analysis (correlation coefficients −0.53) (Table 3). Recent reports demonstrated that miR-21 is a major miRNA that may play an oncogenic role in lung carcinogenesis (Volinia et al, 2006; Yanaihara et al, 2006; Seike et al, 2009). The expression levels of miR-21 were examined by real-time quantitative RT–PCR. miR-21 expression was significantly higher in sensitive cells than in resistant cells (P<0.05, paired t-test) (Figure 3F). The quantitative comparison of miR-21 and JAK1 showed a significant positive correlation between these two (Pearson's correlation, r=0.67, P<0.05) (Figure 3G). We ultimately recognised JAK1, VEGFR2, VEGFR3 and miR-21 as factors concerned with sensitivity to enzastaurin. In particular, JAK1 is the most significant molecule involved in drug response.
JAK1 expression effect on drug sensitivity in A549 cells
To investigate further the effect of JAK1 on sensitivity to enzastaurin, JAK1 protein expression of 11 NSCLC cells was evaluated by western blot analysis. Elevated JAK1 protein was observed in enzastaurin-sensitive NSCLC cells (Figure 4A). Next, we inhibited JAK1 protein using JAK1 inhibitor in enzastaurin-sensitive A549 and RERF-LC-KJ cells. After the treatment of JAK inhibitor (1 μ M), JAK1 and its downstream p-STAT3 expression was completely diminished until 72 h in A549 cells (Figure 4B). We examined the effect of enzastaurin and JAK inhibitor combination therapy on cell growth. Concurrent JAK inhibitor and enzastaurin therapy significantly decreased the growth-inhibitory effect of enzastaurin, compared with enzastaurin monotherapy in enzastaurin-sensitive A549 cells (Figure 4C). Enzastaurin therapy after JAK inhibitor 1 μ M treatment also diminished the growth-inhibitory effect of enzastaurin, compared with enzastaurin monotherapy in A549 cells (Figure 4D). The IC50 values of concurrent enzastaurin with JAK inhibitor and enzastaurin therapy after JAK inhibitor were 76 and 83, respectively, whereas that of enzastaurin monotherapy was 5.8 (Figures 4C and D). In addition, RERF-LC-KJ cells, which are also sensitive to enzastaurin, showed resistance after JAK inhibitor therapy in combination with enzastaurin (data not shown). In RERF-LC-KJ cells, both IC50 values of concurrent enzastaurin with JAK inhibitor and enzastaurin therapy after JAK inhibitor were over 100, whereas that of enzastaurin monotherapy was 6.8. To confirm further the ability of JAK1 to indicate drug sensitivity to enzastaurin, we developed a lentiviral vector for the expression of JAK1 and established stable JAK1-overexpressing A549 cells (LV-JAK1-A549 cells). Western blot analysis showed the overexpression of JAK1 in LV-JAK1-A549 cells (Figure 4E). The growth-inhibitory effect of enzastaurin on LV-JAK1-A549 cells was assessed by MTS assay. The drug sensitivities of two LV-JAK1-A549 cells were greater than those in the control cells (Figure 4F). The IC50 values of two LV-EGFP A549 cells were 2.2 and 4.5, respectively, whereas that of LV-EGFP A549 cells was 25 (Figure 4F). These results indicate that JAK1 expression contributed to the drug sensitivity and could be used as a drug-sensitive marker to enzastaurin in lung cancer cells.
JAK/STAT3 pathway directly activates miR-21
A significant correlation between JAK1 and miR-21 was found in our set of NSCLC cells (Figure 3G). STAT3 is a transcription factor activated by JAK1, and its binding to the target sites in miR-21 promoter upon IL-6 induction has been reported previously (Löffler et al, 2007; Iliopoulos et al, 2010). To verify the association between JAK1 and miR-21, miR-21 expression was quantified after the stimulation of IL-6 by qRT–PCR analysis. Upon IL-6 exposure, p-STAT3 expression was significantly upregulated, resulting in the overexpression of miR-21 at 24 h in A549 cells (Figures 5A and B). We also evaluated the miR-21 expression in LV-LAK1 A549 cells. In the JAK1-overexpressing cells, miR-21 expression was significantly higher than in parent cells (Figure 5C). These results supported the concept that miR-21 is directly induced by JAK/STAT signalling in NSCLC cells.
Discussion
Enzastaurin has recently been evaluated as second- or third-line therapy of NSCLC in a phase II study (Oh et al, 2008; Chiappori et al, 2010). Synergistic effects of the combination of enzastaurin and cytotoxic drugs including cisplatin, gemcitabine and pemetrexed have been found in NSCLC cells in an in vitro study (Rademaker-Lakhai et al, 2007; Morgillo et al, 2008; Tekle et al, 2008). A recent study showed that enzastaurin inhibited in vivo metastasis of NSCLC cells (Körner et al, 2010). It is known that PKCs mediate the regulation of the cell cycle; enzastaurin is also able to inhibit several proteins involved in cell-cycle regulation, for example, E2F-1 associated with G1/S checkpoint and Cdc25C resulting in G2/M checkpoint (Tekle et al, 2008). These checkpoint arrests provide the tumour cells with the opportunity to repair their DNA, which has been damaged by cytotoxic drugs. Reduction of E2F-1 expression and phosphorylated Cdc25C by enzastaurin might explain the abrogation of the checkpoint arrest and could facilitate cytotoxic drug-damaged cells to undergo apoptosis. Furthermore, a recent study demonstrated that enzastaurin had a cooperative effect with gefitinib and was able to revert gefitinib resistance in cancer cells through the inhibition of Akt and VEGF pathways (Gelardi et al, 2008). These studies suggest that enzastaurin might be a promising novel agent in NSCLC patients.
Enzastaurin inhibited the downstream PKCβ signalling, PI3K/AKT pathway and the phosphorylation of glycogen synthase kinase-3β (Keyes et al, 2002; Graff et al, 2005). Anti-tumour and anti-angiogenic activity of enzastaurin was also demonstrated in tumour xenograft models, including NSCLC, and was confirmed using a standardised clonogenic assay in patient-derived tumour explants (Keyes et al, 2004). Significant reduction of VEGF protein levels following enzastaurin treatment, together with a significant decrease in intratumoural vessel density, has been demonstrated in vivo (Keyes et al, 2004). In the current study using a RTKs phosphorylation antibody array, we found elevated levels of VEGFR2 and VEGFR3 in the enzastaurin-sensitive cells. Our results are in agreement with previous data concerning enzastaurin and anti-angiogenic activity. These findings demonstrated that lung cancer cases with activated angiogenic activity should respond to enzastaurin treatment.
In this study, using gene-chip and pathway analysis, we identified 16 genes that correlated with sensitivity to enzastaurin. Pathway analysis also revealed that JAK1 was the most important molecule affected by enzastaurin treatment of NSCLC. The JAK is a non-RTK and can activate STAT3 transcriptional factor. The STAT3 is also persistently activated in about half of NSCLC tumours and is involved in tumour invasion, metastasis and angiogenesis through differential gene regulation (Haura et al, 2005; Song et al, 2011). Increased levels of JAK1 and STAT3 were observed in the sensitive cells in this study. Knockdown of JAK resulting in p-STAT3 also diminished the growth-inhibitory effect of enzastaurin in the sensitive cells. In contrast, overexpression of JAK1 by lentiviral-mediated production enhanced the drug sensitivity to enzastaurin in the sensitive cells. These results suggest that JAK expression levels can be used as predictive markers of enzastaurin sensitivity. Non-small-cell lung cancer patients with an activated JAK/STAT3 pathway are suitable cases for enzastaurin treatment.
MicroRNAs are small non-coding RNA molecules of about 20 nucleotides that are frequently located at chromosomal regions deleted or amplified in cancers, suggesting that miRNAs are a new class of genes involved in human tumourigenesis (Lu et al, 2005; Volinia et al, 2006; Yanaihara et al, 2006; Seike et al, 2009). Recently, miRNAs have been demonstrated as diagnostic and prognostic markers in lung cancer (Yanaihara et al, 2006; Seike et al, 2009). We previously reported that the inhibition of miR-21, whose upregulation is associated with EGFR mutations, can be a therapeutic strategy, either as a monotherapy or in combination with EGFR-TKI treatment (Seike et al, 2009). In this study, expression of miR-21 and its host gene, TMEM49, were significantly higher in enzastaurin-sensitive cells than in enzastaurin-resistant cells. In addition, a significant positive correlation was observed between miR-21 and JAK1. The STAT3 reportedly signals IL-6-induced upregulation of miR-21 in multiple myeloma cells (Löffler et al, 2007). We confirmed that JAK1 and its downstream target STAT3, containing three binding sites of miR-21 promoter, directly activated miR-21 in NSCLC cells. These results suggest that, in lung cancer, miR-21 affects the response to enzastaurin through the JAK/STAT signalling pathway.
In conclusion, we have identified unique molecules; genes, RTKs and miRNAs that are correlated with sensitivity to enzastaurin and have constructed an eight-gene signature to distinguish the sensitive cells from the resistant cells. Furthermore, we demonstrate that JAK1 is the most significant factor concerned in response to enzastaurin. Patient selection based on the JAK expression might be useful for future clinical development of enzastaurin therapy in NSCLC.
Change history
29 March 2012
This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication
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Acknowledgements
This study was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and Basic and Clinical Studies on Functional RNA Molecules for Advanced Medical Technologies (to MS and AG).
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Shimokawa, T., Seike, M., Soeno, C. et al. Enzastaurin has anti-tumour effects in lung cancers with overexpressed JAK pathway molecules. Br J Cancer 106, 867–875 (2012). https://doi.org/10.1038/bjc.2012.7
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DOI: https://doi.org/10.1038/bjc.2012.7
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