Segmental chromosomal alterations lead to a higher risk of relapse in infants with MYCN-non-amplified localised unresectable/disseminated neuroblastoma (a SIOPEN collaborative study)

Abstract

Background:

In neuroblastoma (NB), the presence of segmental chromosome alterations (SCAs) is associated with a higher risk of relapse.

Methods:

In order to analyse the role of SCAs in infants with localised unresectable/disseminated NB without MYCN amplification, we have performed an array CGH analysis of tumours from infants enroled in the prospective European INES trials.

Results:

Tumour samples from 218 out of 300 enroled patients could be analysed. Segmental chromosome alterations were observed in 11%, 20% and 59% of infants enroled in trials INES99.1 (localised unresectable NB), INES99.2 (stage 4s) and INES99.3 (stage 4) (P<0.0001). Progression-free survival was poorer in patients whose tumours harboured SCA, in the whole population and in trials INES99.1 and INES99.2, in the absence of clinical symptoms (log-rank test, P=0.0001, P=0.04 and P=0.0003, respectively). In multivariate analysis, a SCA genomic profile was the strongest predictor of poorer progression-free survival.

Conclusion:

In infants with stage 4s MYCN-non-amplified NB, a SCA genomic profile identifies patients who will require upfront treatment even in the absence of other clinical indication for therapy, whereas in infants with localised unresectable NB, a genomic profile characterised by the absence of SCA identifies patients in whom treatment reduction might be possible. These findings will be implemented in a future international trial.

Main

In neuroblastoma (NB), the most frequent solid extracranial cancer in childhood, characterised by substantial clinical heterogeneity, several recurrent genetic alterations have been shown to be of prognostic impact (Maris et al, 2007; Janoueix-Lerosey et al, 2010; Maris, 2010). A near-triploid DNA content, on average reflecting whole chromosome gains, is frequently observed in low-stage tumours of younger children, and is associated with a favourable outcome (Ladenstein et al, 2001). On the other hand, MYCN amplification and segmental chromosome alterations (SCAs) most often involving chromosome regions 1p, 1q, 2p, 3p, 4p, 11q and 17q are preferentially observed in advanced stages of disease in older children, and are associated with a poorer prognosis. These genetic parameters can now be analysed using genome-wide techniques such as array CGH or SNP arrays, clearly demonstrating that the genetic imbalances combine to define distinct genomic profiles (Vandesompele et al, 2005; George et al, 2007; Mosse et al, 2007; Schleiermacher et al, 2007; Tomioka et al, 2008; Janoueix-Lerosey et al, 2009). Indeed, the presence of SCAs, even in a background of numerical chromosome alterations (NCAs), is associated with a higher risk of relapse and a poorer outcome (Janoueix-Lerosey et al, 2009). Although risk stratification schemes have so far integrated molecular data based on only few chromosome loci (Cohn et al, 2009), more recent reports suggest that pangenomic data could further improve pre-therapeutic risk estimation (Ambros et al, 2009; Janoueix-Lerosey et al, 2009; Caren et al, 2010). As high-risk NBs nearly always demonstrate SCA, future therapeutic strategies for these cases might rather rely on gene expression or other molecular data (Oberthuer et al, 2008; Vermeulen et al, 2009; Ambros et al, 2011). However, pangenomic data might prove to be especially informative for treatment stratification in the clinically defined low- and intermediate-risk groups (Janoueix-Lerosey et al, 2009; Schleiermacher et al, 2010). To date, the exact role of genomic imbalances in infants, particularly with localised unresectable/disseminated NB, has not been reported.

The aim of this study was to analyse genetic alterations determined by array CGH in MYCN-non-amplified localised unresectable/disseminated NB of infants included in the prospective European INES99.1, INES99.2 and INES99.3 protocols (De Bernardi et al, 2009; Rubie et al, 2011) and to study the impact of the genomic profile on clinical characteristics and outcome in this population.

Patients and methods

Patients

Tumour samples from patients included in the INES99.1, INES99.2 and INES99.3 trials were included in this study (De Bernardi et al, 2009; Rubie et al, 2011) The INES trials, run by the Société Internationale d’Oncologie Pédiatrique–Europe Neuroblastoma (SIOPEN) in the participating countries Austria, Belgium, France, Italy, Norway, Portugal, Spain, Sweden and United Kingdom, recruited 300 infants aged <12 months diagnosed with a MYCN-non-amplified NB from 1999 to 2004. The INES99.1 trial proposed minimal upfront chemotherapy in infants with a localised unresectable NB with the aim to render these tumours resectable (Cecchetto et al, 2005; Rubie et al, 2011). The INES99.2 and INES99.3 trials proposed chemotherapy for infants with a disseminated NB, in case of life- or organ-threatening symptoms only in stage INSS 4s, or in case of metastases to the bone, lung or CNS (stage INSS 4), respectively (De Bernardi et al, 2009). Surgical resection of the primary tumour was performed in the absence of surgical risk factors (Cecchetto et al, 2005). In case of disease progression or relapse, individual therapeutic decisions were taken. The protocol was approved by local institutional review boards, and patients were enroled following written informed consent from parents or guardians. Median follow-up of these 300 patients was 60 months; 36 patients have had disease progression or relapse, and 9 patients have died (0 out of 119 in INES99.1, 7 out of 133 in INES99.2 and 2 out of 48 in INES99.3), with a 5-year progression-free survival (PFS) and overall survival (OS) of 87.8% (±1.9) and 97.5% (±0.9), respectively.

Pangenomic profile

For a total of 218 out of 300 patients, array CGH was performed using DNA extracted from frozen tumour tissue obtained at diagnosis and harbouring >50% tumour cells. Tumours from 93 out of 119 INES99.1, 93 out of 133 INES99.2 and 32 out of 48 INES99.3 patients could be analysed, with array CGH analysis results of 57 samples from French patients having been reported previously (Janoueix-Lerosey et al, 2009; Schleiermacher et al, 2010). For the remaining patients, either no tumour tissue was available or samples contained an insufficient amount of tumour cells or yielded poor-quality DNA not permitting interpretation of the array CGH result. The absence of MYCN amplification was confirmed by fluorescent in situ hybridisation in a national SIOPEN reference laboratory and centrally reviewed for all cases (Ambros et al, 2009). Among the 218 patients, there have been 30 relapses/progressions, and 5 patients have died.

Following standardised DNA extraction, samples were analysed by array CGH using for 185 cases an in-house BAC/PAC array with a genomic resolution of 1 Mb, as reported previously (Janoueix-Lerosey et al, 2009; Schleiermacher et al, 2010). Using a commercially available NimbleGen DNA array (Roche NimbleGen, Madison, WI, USA), 33 other cases were analysed, containing 72 000 oligonucleotide probes, with an average resolution of 1 probe per 40 kb.

All obtained profiles were subjected to detailed visual inspection and analysed using the VAMP software (La Rosa et al, 2006). The smoothing algorithm GLAD (Hupe et al, 2004) was used to determine the status of the BAC or oligonucleotide probes (normal or altered ratios). A NCA was defined as probe ratios homogeneously altered throughout entire chromosomes, as compared with the median copy number across the genome. A SCA was defined by the presence of either at least 3 contiguous BAC or 100 contiguous oligonucleotide probes exhibiting a genomic status different from that of the rest of the chromosome.

A genomic type was attributed to all analysed samples, taking into account all observed genomic alterations, as described previously, with slight modifications (Janoueix-Lerosey et al, 2009; Schleiermacher et al, 2010). Cases presenting only NCA, without any SCA, were considered as having a ‘NCA genomic profile’. Cases harbouring SCA, without or with NCA, were considered as having a ‘SCA genomic profile’. Finally, cases in which no genetic changes were observed despite sufficient tumour cell content in the samples were termed ‘silent’ profiles.

The genomic profiles have been deposited in the NCBI Gene Expression Omnibus (Edgar et al, 2002) and are accessible through GEO Series accession number GSE26494 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26494).

DNA index

The cellular DNA content could be determined by flow or static cytometry in 108 cases. Tumours were classified as di-/tetraploid if the DNA index was 1.2 or 1.8. Tumours with a DNA index between 1.2 and 1.8 were termed pseudotriploid (Ladenstein et al, 2001).

Statistical analysis

Progression-free survival was defined as the time from diagnosis to first event (local or metastatic failure, either during treatment or after completion of treatment) or last follow-up. In patients with INSS stage 4s disease without any specific treatment, progression was taken into account when occurring after 2 months of initial observation. Overall survival was defined as the time from diagnosis to death or last follow-up. Survival curves were analysed according to the Kaplan–Meier method and compared using the log-rank test. Multivariate analysis was performed using the Cox proportional-hazards regression model. Categorical variables were coded as a set of binary ‘yes-no’ variables. A backward model-building procedure was used to identify the variables retained in the Cox model with a P-value of 0.05.

Results

Pangenomic profiling

Of the 218 tumour samples analysed by array CGH, no copy number alterations could be detected in 8 cases. Among the remaining 210 cases, a NCA genomic profile was observed in 162 cases, whereas 48 tumours presented a SCA genomic profile (Figure 1). All cases with a SCA genomic profile harboured imbalances of chromosome regions recurrently altered in NB (gain of chromosome arms 1q, 2p or 17q, loss of chromosome arms 1p, 3p, 4p, 11q), except two cases showing imbalances in 14q, and 4q and 6p, respectively.

Figure 1
figure1

Frequencies of genome copy number gains (y axis, positive values, red) and losses (y axis, negative values, green) at each locus of the CGH array. Clones are ordered on the x axis according to their position in the genome. Vertical grey lines correspond to chromosome separators. (A) Cases with a NCA genomic profile (n=162): presence of numerical chromosome alterations (NCAs) only. (B) Cases with a SCA genomic profile (n=48): presence of segmental chromosomal alterations (SCAs) without or with NCA. The colour reproduction of this figure is available at the British Journal of Cancer online.

Among the 48 cases with a SCA genomic profile, the most frequent SCAs were gain of 17q (81%), gain of 2p (43%), loss of 1p (41%) and loss of 11q (39%) (Figure 1 and Table 1). For most chromosome arms harbouring recurrent imbalances, the breakpoints were scattered over large regions. However, for the 19 tumours harbouring distal 11q loss, breakpoints clustered within a smaller region of 12 Mb (genome position 70–82 Mb; Supplementary Table 1 and Figure 2).

Table 1 Frequency of segmental chromosome alterations and genomic profiles according to the study groups and clinical characteristics
Figure 2
figure2

Localisation of breakpoints observed in 48 tumours harbouring SCA. Each breakpoint was localised according to the coordinates of the probe determining the left side of the breakpoint region, according to the Human Genome Draft Hg18 (genome.ucsc.edu/goldenPath/hgTracks.html). The graph was drawn using the web tool Idiographica (http://www.ncrna.org/idiographica/).

The frequency of the different genomic profiles was analysed in the different study groups, and their distribution in the different groups was not random. Indeed, in INES99.1, 11% of patients had SCA vs 20% in INES99.2 and 59% in INES99.3 (χ2-test, P<0.0001; Table 1). In patients with skin, liver or bone marrow metastases, the frequency of SCA was not significantly higher than in patients without such metastases (Figure 3 and Table 1). However, the single chromosome alterations 1p loss, 2p gain, 3p loss, 4p loss, 11q loss and 17q and a SCA genomic profile were observed more frequently in infants with radiologically confirmed bone metastases compared with those without bone lesions (χ2-test, P<0.0001; Table 1).

Figure 3
figure3

Frequency of genomic types according to the metastatic status (colour codes: dark grey: no genomic alterations; grey: NCA genomic profile; light grey: SCA genomic profile). Here, 38 out of 125 patients with metastases, 7 out of 26 patients with skin metastases and 23 out of 96 patients with liver metastasis had a SCA genomic profile vs 10 out of 93 patients without any metastases, 41 out of 192 patients without skin metastases and 25 out of 122 patients without liver metastases (χ2 test, all NS). Finally, 17 out of 26 patients with radiologically defined bone metastases had a SCA genomic profile vs 31 out of 185 patients without bone metastasis (χ2, P<0.0001).

Among the 108 cases for which ploidy data were available, 33 tumours were diploid/tetraploid and 75 pseudotriploid. The SCA profiles were observed more frequently, but not exclusively, in di/tetraploid tumours (χ2-test, P<0.0001; Table 2).

Table 2 Repartition of the genomic profiles according to ploidy

Survival analysis

The 5-year PFS and OS for the 218 patients were 86.2% (±2.3) and 97.6% (+/1.0), respectively. Among the 5 patients who died of disease, 1 INES99.2 patient with a NCA genomic profile had bone, bone marrow and liver relapse 14 months after diagnosis, and died of disease 35 months after diagnosis, and 1 INES99.2 neonate with a NCA genomic profile died of fulminant disease shortly after diagnosis. Three other patients (2 INES99.2 and 1 INES99.3) with SCA genomic profiles had bone marrow/liver, bone/bone marrow and bone/skin relapse, and died of disease 10, 10 and 46 months after diagnosis, respectively (Table 3).

Table 3 Patients having relapsed, according to the protocol arm and genomic profile

Further survival analyses concerned PFS only. The genetic markers 2p gain, 11q loss and 17q gain were all associated with a poorer PFS (Table 4). A SCA genomic profile was also strongly associated with a poorer PFS (Table 4 and Figure 4). Infants with a SCA genomic profile had a poorer PFS than those with a NCA genomic profile. Interestingly, the eight infants whose tumours had silent genomic profiles also fared worse.

Table 4 Prognostic impact of single genetic alterations and genomic profiles
Figure 4
figure4

Kaplan–Meier survival analysis showing 5-year progression-free survival (PFS) according to the tumour genomic profile. (A) Among the whole study population (n=218), the 5-year PFS was 92% (±2.1) in patients with a NCA genomic profile, 70.7% (±6.6) in patients with a SCA genomic profile and 62.5% (±17.1) in patients with a silent genomic profile (log-rank, P=0.0001). (B) Among infants with a localised unresectable NB (INES99.1), the 5-year PFS was 91% (±3.2) in patients with a NCA genomic profile vs 70% (±14.5) in patients with a SCA genomic profile (log-rank, P=0.04). (C) Among infants with stage 4s NB (INES99.2), the 5-year PFS was 91.7% (±3.2) in patients with a NCA genomic profile vs 7% (±11.5) in patients with a SCA genomic profile (log-rank, P=0.0003). (D) Among infants with stage 4 NB (INES99.3), the 5-year PFS was 100% in patients with a NCA genomic profile vs 84.2% (±8.3) in patients with a SCA genomic profile (log-rank, not significant). (E) Among infants with stage 4s NB receiving upfront chemotherapy, the 5-year PFS was 86.2% (±6.4) in patients with a NCA genomic profile vs 80.8% (±12.2) in patients with a SCA genomic profile (log-rank, NS). (F) Among infants with stage 4s NB not receiving upfront chemotherapy, the 5-year PFS was 95.3% (±3.2) in patients with a NCA genomic profile vs 25% (±15.3) in patients with a SCA genomic profile (log-rank, P<0.0001).

A SCA genomic profile was associated with a poorer PFS in infants with localised unresectable (INES99.1) and with stage 4s NB (INES99.2) (log-rank, P=0.04 and P=0.0003, respectively). Finally, among infants with stage 4 disease (INES99.3), no statistically significant difference in PFS between patients with a NCA and SCA genomic profile was observed (Figure 4A–D). The DNA index was not of prognostic impact in the studied population.

Multivariate analysis

To determine which parameters independently predicted PFS, we applied the Cox proportional-hazards procedure including the 210 patients in whose tumours either NCA or SCA profiles had been identified, entering the variables genomic profile, single genetic alterations and treatment group. In a backward model, a SCA genomic profile was found to have a higher risk of relapse (hazard ratio: 5.24, CI 2.4–11.4, P<0.0001), whereas a lower risk of relapse was observed for treatment group INES99.3 (hazard ratio: 0.32, CI 0.094–1.11, P=0.076). The single genetic alterations were not retained in the model.

Prognostic impact of genomic profiling in stage 4s patients

In patients with stage 4s disease (trial INES99.2), treatment can be very heterogeneous, with a possibility of observation only, in the absence of clinical symptoms, or necessity of upfront chemotherapy in the presence of clinical symptoms, leading to very different total treatment burdens. We thus sought to analyse the impact of the genomic profile on treatment burden. Among 91 patients with stage 4s disease, 40 patients had symptoms at diagnosis and thus received upfront medical treatment. In this group, no significant difference between PFS of the patients with NCA and typical SCA genomic profiles could be observed. However, for the 51 patients who did not have clinical symptoms at diagnosis and who initially did not receive upfront medical treatment, those with a SCA genomic profile had a significantly lower PFS than those with a NCA genomic profile (Figure 4E and F).

Indeed, for stage 4s patients, both among patients with NCA and with SCA tumours, 50% of the patients (43 out of 72 and 8 out of 19 patients, respectively; χ2 test not significant) did not require upfront chemotherapy, but had medical observation±surgical resection only, indicating that the clinical severity of disease was not worse at diagnosis in the SCA tumour group. However, once having relapsed, only 2 out of 6 (33%) patients with a NCA profile received high-dose chemotherapy for salvage, whereas in the SCA tumour group, 5 out of 8 (62%) patients received such treatment (Table 3).

Discussion

In NB, different recurrent genetic alterations combine to form distinct genomic types, which are in turn associated with different clinical outcomes. We have recently shown in a large patient cohort that the presence of SCA is associated with a poorer outcome, even when occurring together with NCA, and that tumour progression is frequently associated with an accumulation of SCA, suggesting that SCA could be considered as surrogate markers for an underlying abnormality in a DNA maintenance or repair pathway (Janoueix-Lerosey et al, 2009; Schleiermacher et al, 2010). We have now explored the hypothesis that in infants with MYCN-non-amplified localised unresectable/disseminated NB, pangenomic profiling might provide a useful prognostic marker.

In infants with NB without MYCN amplification, overall survival is, fortunately, high. However, in this patient population with a frequent indication for treatment even in newborns, it is crucial to consider treatment burden, as conventional chemotherapy courses, well tolerated in older children, can be associated with significant short- or long-term morbidity. It is thus important to fine-tune treatment indications for these patients in order to avoid over- or under-treatment. Considering that patients of this study have an excellent OS as previously reported (De Bernardi et al, 2009; Rubie et al, 2011), this study used PFS to determine if pangenomic profiling might be useful for therapeutic stratification in infants with MYCN-non-amplified localised unresectable/disseminated NB.

This study confirms recurrent SCA involving chromosome arms 1p, 1q, 2p, 3p, 4p, 11q and 17q in NB, breakpoints in other chromosome arms being much rarer. The data are concordant with previous publications reporting a lower frequency of 1p deletion, 11q deletion or 17q gain in infants with localised NB, and frequencies of 17q gain ranging from 50 to 70% in infants with stage 4s or 4 disease (Spitz et al, 2006; Lavarino et al, 2009). However, previous studies did not take into account the whole genomic profile. We now show that a genomic profile characterised by the presence of SCA occurs in 11% of infants with localised unresectable NB, 20% of infants with stage 4s and 59% of infants with stage 4 NB. A higher incidence of SCA was observed in older infants, and the median age at diagnosis was higher in infants with a SCA than those with a NCA genomic profile (6.7 vs 5.1 months at diagnosis, t-test, P=0.006).

In infants with disseminated NB, the exact clinical delineation of INSS stage 4s vs stage 4 has been controversial (Brodeur et al, 1993; Hero et al, 2008; Cohn et al, 2009; De Bernardi et al, 2009). It has been reported that infants with stage 4s disease, including those with a primary tumour crossing the midline, or those with skeletal MIBG uptake in the absence of radiologically proven bone lesions, will require chemotherapy only in the presence of clinical symptoms (De Bernardi et al, 2009). We now show that in infants with NB, a SCA genomic profile is associated with metastatic disease and, in particular, bone metastasis. Furthermore, among 185 patients without bone metastasis at diagnosis, 3 out of 154 with a NCA genomic profile progressed with bone lesions vs 6 out of 31 with a SCA genomic profile. This observation leads to the hypothesis that the presence of SCA in NB cells might potentially increase their potential to metastasise to bone.

It has been suggested that 11q deletions might be associated with a particularly dismal outcome in older patients (Attiyeh et al, 2005; Caren et al, 2010). In this study, of the 5 patients who have died of disease, 2 had 11q deletion; and among 17 other patients whose tumours harboured 11q deletion, 5 have relapsed and could be salvaged, indicating that 11q deletion is not associated with a worse OS in infants.

Several recent studies, performed in large patient cohorts encompassing NB patients of all ages, have demonstrated that a genomic profile characterised by SCA is associated with a higher risk of relapse (Tomioka et al, 2008; Janoueix-Lerosey et al, 2009; Caren et al, 2010). We now show specifically in infants with MYCN-non-amplified localised unresectable/disseminated NB, in a multivariate setting, that a SCA genomic profile is of prognostic importance, rather than single genetic alterations or clinical stage. No correlation between the size of the individual chromosome alterations and clinical outcome was observed (data not shown). The absence of prognostic impact of the clinical stage in a multivariate setting can probably be attributed to the more intensive chemotherapy for INES99.3 (INSS stage 4) patients, indicating that these patients are sufficiently treated with 4–8 courses of chemotherapy (De Bernardi et al, 2009; Baker et al, 2010). The type of SCA to be taken into account for the definition of a SCA genomic profile remains controversial, with some recent data supporting the hypothesis that any SCA, whether occurring recurrently or not in NB, may be associated with a poor outcome (Janoueix-Lerosey et al, 2009). In this series, only two tumours with a SCA genomic profile harboured only atypical imbalances, precluding from drawing any conclusion on their prognostic impact.

Interestingly, the infants with silent genomic profiles appeared to have a higher risk of relapse. For one sample, a DNA index of 1.46 was noted, indicating a perfect triploid chromosome content, in the context of which NCA might not be detected. On the other hand, it cannot be excluded that smaller alterations not detected by standard resolution arrays might be present in some NBs. The tumours of these patients will merit further exploration using higher-resolution techniques to search for as yet undetected genetic alterations.

Recent studies have enabled the identification of genomic loci associated with NB susceptibility at a constitutional level. These loci have been identified by genome-wide association studies (GWASs), using high-resolution SNP arrays. Common variants within the FLJ22536, BARD1 and LMO1 genes are significantly associated with susceptibility to high-risk NB, whereas SNPs within DUSP12, DDX4 and IL31RA are associated with less aggressive NB (Maris et al, 2008; Capasso et al, 2009; Diskin et al, 2009; Nguyen le et al, 2011; Wang et al, 2011). Constitutional copy number variations associated with NB susceptibility have been described for LMO1 at 11p15.4 as well as the NBPF23 gene at 1q21.1 (Diskin et al, 2009; Wang et al, 2011). For the former, somatic copy number changes as whole chromosome arm 11p gain were observed in 12% of tumours, but only 5% showed interstitial gain of 11p15. In our study, focusing on genomic profiling of tumour DNA, the lower-resolution array CGH analysis did not detect any copy number alterations of interstitial chromosome regions surrounding the known susceptibility loci 1q21.1 and 11p15.4.

The DNA index was not of prognostic impact in this study using previously published cutoffs. The exact definition of diploid vs pseudotriploid tumours based on DNA index remains controversial (Look et al, 1984; Ladenstein et al, 2001). When using a more restrictive DNA index cutoff of 1 to define diploid tumours, these 11 cases had a poorer PFS compared with the 97 others. However, a SCA genomic profile was associated with a higher risk of relapse even among patients whose tumours had a DNA index of >1 (P=0.002).

The prognostic impact of the genomic profile in infants with localised unresectable/disseminated NB without MYCN amplification is of high clinical importance. Indeed, the presence of a SCA genomic profile identifies stage 4s NB patients with a higher risk of progression or relapse, for whom salvage therapy might have to be more intense, justifying more upfront treatment in patients who would otherwise receive little or no treatment (De Bernardi et al, 2009). On the other hand, a NCA genomic profile defines a population of infants with localised unresectable NB with a lower risk of disease progression. Thus, for infants with localised unresectable NB with a NCA profile, treatment reduction might be possible (Hero et al, 2008). These findings will be implemented in a future international trial.

Accession codes

Accessions

Gene Expression Omnibus

Change history

  • 29 March 2012

    This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication

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Acknowledgements

In France, this work was supported by grants from the Institut National de la Santé et de la Recherche Médicale, the Ligue Nationale Contre le Cancer (Equipe labellisée) and the PHRC AOM 02014. The construction of the BAC/PAC array was supported by grants from the Carte d’Identité des Tumeurs programme of the Ligue Nationale Contre le Cancer. We thank the associations APAESIC (Association des Parents et des Amis des Enfants Soignés à l’Institut Curie), Association Hubert Gouin, ‘Les Bagouz à Manon’ and ‘Enfants et Santé’. We also thank Ms Sandrine Damerval-Ferrand for excellent technical assistance and Philippe La Rosa for help in the preparation of figures. In Spain, this work was furthermore supported by the following grants: RD06/0020/0102 from RTICC, ISCIII and ERDF and 396/2009 from FAECC. In Italy, RD and KM are fellows of the Italian Neuroblastoma Foundation. We also thank the Italian Neuroblastoma Foundation.

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Correspondence to G Schleiermacher.

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Schleiermacher, G., Michon, J., Ribeiro, A. et al. Segmental chromosomal alterations lead to a higher risk of relapse in infants with MYCN-non-amplified localised unresectable/disseminated neuroblastoma (a SIOPEN collaborative study). Br J Cancer 105, 1940–1948 (2011) doi:10.1038/bjc.2011.472

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Keywords

  • neuroblastoma
  • infants
  • genomic profile
  • segmental chromosome alterations
  • prognosis

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