Phosphorylation of Akt on Ser473 in NSCLC tumour tissue in comparison with patient-matched normal lung tissue. Samples were western blotted and the data quantified exactly as described in Figure 1. (A) The extent of Ser473 phosphorylation in normal (N1–3; light grey) or tumour (T1–3; black) tissue for each patient (mean±s.e.m.; *P<0.05). (B) The percentage changes in Akt Ser473 phosphorylation in tumour samples in comparison with patient-matched normal tissue with patients are ranked according to the percentage change in phosphorylation.