Abstract
Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sensitive but fibroblastic cells (BHK 21/C13/DWS-3) grown under identical conditions are relatively insensitive. This study has established that bleomycin is a powerful anti-mitotic agent during the G2 phase but it does not prevent prophase cells from completing a normal mitotic division. Its ability to suppress DNA synthesis contributes little to the inhibition of cell cycling.
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Wheatley, D., Mueller, G. & Kajiwara, K. Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle. Br J Cancer 29, 117–131 (1974). https://doi.org/10.1038/bjc.1974.48
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DOI: https://doi.org/10.1038/bjc.1974.48
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