Differential depth of treatment response required for optimal outcome in patients with blast phase versus chronic phase of chronic myeloid leukemia

The outcome of patients with chronic myeloid leukemia (CML) has improved dramatically following the introduction of tyrosine kinase inhibitor (TKI) therapy. 1 With TKI treatment, most patients with CML, chronic phase (CML-CP) can achieve complete cytogenetic response (CCyR) within 12 months after initial diagnosis of CML. Thanks to the high sensitivity of molecular methods to detect BCR-ABL1 transcripts, deeper molecular responses can now be ascertained, including major molecular response (MMR), MR4 or MR4.5 (4 and 4.5- log reduction of BCR-ABL1 fusion transcripts, respectively), and molecularly undetectable leukemia (MUL). 2,3 It seems intuitive that the deeper the treatment response, the better the survival of patients with CML-CP is. 4,5 However, multiple studies have shown that deeper responses beyond CCyR confer no survival bene ﬁ t. Patients with CML-CP who achieve CCyR have a very favorable survival similar to that of the general population. 6,7 In contrast, CML-CP patients who are unable to achieve CCyR have a signi ﬁ cantly poorer survival, suggesting that achieving CCyR is essential for predicting a favorable outcome. In the pre-TKI era, in most patients the disease progressed to blast phase (BP) within 3 – 4 years after initial diagnosis of CML-CP.

The outcome of patients with chronic myeloid leukemia (CML) has improved dramatically following the introduction of tyrosine kinase inhibitor (TKI) therapy. 1 With TKI treatment, most patients with CML, chronic phase (CML-CP) can achieve complete cytogenetic response (CCyR) within 12 months after initial diagnosis of CML. Thanks to the high sensitivity of molecular methods to detect BCR-ABL1 transcripts, deeper molecular responses can now be ascertained, including major molecular response (MMR), MR4 or MR4.5 (4 and 4.5-log reduction of BCR-ABL1 fusion transcripts, respectively), and molecularly undetectable leukemia (MUL). 2,3 It seems intuitive that the deeper the treatment response, the better the survival of patients with CML-CP is. 4,5 However, multiple studies have shown that deeper responses beyond CCyR confer no survival benefit. Patients with CML-CP who achieve CCyR have a very favorable survival similar to that of the general population. 6,7 In contrast, CML-CP patients who are unable to achieve CCyR have a significantly poorer survival, suggesting that achieving CCyR is essential for predicting a favorable outcome.
In the pre-TKI era, in most patients the disease progressed to blast phase (BP) within 3-4 years after initial diagnosis of CML-CP. With TKI therapy, the progression of CML from CP to BP has decreased substantially and the long-term cumulative probability of such progression is currently about 5%. 1,8 Despite the revolutionary progress in the treatment of patients with CML-CP, CML-BP remains a therapeutic challenge. In general, CML-BP is a fatal disease in the era of TKI therapy, with a median survival of only 6-10 months. 9,10 Further investigation into the potential relationship between the depth of treatment response and optimal patient survival is warranted. Here we investigate a large cohort of patients with CML-BP treated in the era of TKIs and determine whether achieving CCyR is adequate for optimal survival or if deeper molecular responses have a prognostic value.
Cases of CML-BP that met following selection criteria were included in this study: 1, CML-BP diagnosed from 2001 to 2016; 2, presence of t(9;22)(q34;q11.2) or variant translocations detected by conventional karyotyping analysis; and 3, available data regarding molecular response beyond CCyR if achieved. Patients who presented with isolated myeloid sarcoma without concurrent BP in the bone marrow or peripheral blood and patients with BCR-ABL1-positive de novo acute leukemia were excluded in the study. The blast phase was defined as 30% or more blasts in the bone marrow or peripheral blood. Overall survival (OS) was calculated from the date of diagnosis of BP to the date of last follow-up or death. The study was approved by the Institutional Review Board of the University of Texas MD Anderson Cancer Center.
In total, 386 patients with CML-BP were included in this study, including 253 (65.5%) patients with myeloid BP (MyBP), 121 (31.3%) patients with lymphoid BP (LyBP) and 12 (3.1%) patients with mixedphenotype BP. There were 252 (65.3%) men and 134 (34.7%) women with a median age of 51.9 years (range: 13.2-90.2 years) at the time of diagnosis of CML-BP. The median interval time from initial diagnosis of CML to onset of BP was 22.5 months (range: 0-238.5 months). After onset of BP, 361 (93.5%) patients received TKI treatment, 311 (80.6%) received chemotherapy and 116 (30.1%) underwent allogeneic stem cell transplantation. The clinical characteristics of the entire cohort and each subgroup are listed in Table 1.
These patients were further stratified into five subgroups based on the depth of treatment response: 1, no hematologic response (HR), which included 141 patients; 2, HR only, which included 94 patients who achieved HR but not CCyR; 3, CCyR only, which included 31 patients who achieved CCyR but not MMR; 4, MMR, which included 28 patients who achieved MMR but not molecularly undetectable leukemia (MUL); and 5, MUL, which included 92 patients (Table 1). Patients who achieved deeper responses beyond MMR but not MUL were lumped with MMR due to low numbers of patients and the test results falling beyond the linearity of our assay once the BCR-ABL1:ABL1 ratio was o0.01%.
The OS of patients with CML-BP was poor ( Figure 1a). Patients with myeloid BP had a worse survival than those with lymphoid BP (P = 0.0004). The median OS was 9.2 months for patients with MyBP, 19.2 months for those with LyBP and 11.4 months for the entire cohort.
We first analyzed the relationship between the depth of treatment response and patient survival of the five subgroups in the entire cohort. There was a significant difference in survival among the five subgroups of patients (Figures 1b, P o 0.0001), correlating with the depth of remission and a general trend between a deeper response and a longer survival. As expected, patients without HR had the worst outcome with a median OS of 3.6 months. In contrast, patients with MUL had the best outcome with a median survival of 132.2 months and 5-year OS of 71.8%. Patients with MMR, CCyR and HR had an intermediate outcome with a median survival of 38.0, 17.6 and 11.0 months, and 5-year OS was 34.4, 12.2 and 11.0%, respectively. There was no significant difference in survival between patients who achieved CCyR and those who achieved HR (P = 0.33).
We then analyzed the relationship between the depth of response and survival in CML patients with MyBP or LyBP separately. As shown in Figures 1c and d, there was a general trend between a better survival and a deeper molecular response in both MyBP and LyBP groups. In both groups, patients who achieved HR had significantly better OS than those who did not achieve HR (P o 0.0001 for both groups), and patients who achieved MUL had a better survival than those who achieved MMR (P = 0.11 for the MyBP group and P = 0.037 for the LyBP group). There was no significant difference between patients who achieved CCyR and those who achieved HR in neither groups. The lack of statistical significance between some of these subgroups pairwise was related, at least in part, to the low numbers of patients, particularly low numbers of patients who achieved CCyR or MMR.
Of note, 80 of 92 (87.0%) CML-BP patients who achieved MUL received allogeneic hematopoietic stem cell transplantation (Table 1), re-enforcing the notion that allogeneic hematopoietic  The data presented here show that the impact of depth of treatment response on survival is different in patients with CML-CP versus patients with CML-BP. Patients with CML-CP who achieve CCyR have a survival more favorable than those who achieve only HR, and their survival is similar to those who achieve MMR and the general population. 6,7 Therefore, achieving MMR, not MUL, is the primary goal of therapy. In contrast, patients with CML-BP who achieve CCyR have a dismal 5-year survival rate similar to those who achieve HR and significantly worse than those who achieve MMR. CML-BP patients who achieve MMR also have a poor 5-year survival rate (34.4% in this study). By contrast, patients with CML-BP who achieve MUL have a much better outcome with a 5-year survival rate of 71.8% and a median OS of 132.2 months in this study.
In an earlier study, Hehlmann et al. 11,12 reported a more favorable survival in CML-CP patients who achieved a deeper molecular response, although MR4.5 versus CCyR had a smaller effect on survival than CCyR versus no CCyR, given the superior survival of those who achieved CCyR. Despite the smaller survival benefit, achieving MR4.5 is clinically meaningful as it may serve as an indicator of a step toward facilitating successful discontinuation of treatment. 12,13 In contrast, CML-BP patients who achieve CCyR have a dismal outcome and the survival advantage of deeper molecular responses (MUL and MMR) versus CCyR is easily appreciated. Thus, it seems true that a deeper response to therapy correlates with a longer survival in both CML-CP and CML-BP patients, albeit to a different degree and with different clinical implications. Interestingly, the outcome of CML-BP patients who achieve CCyR, MMR and MUL somewhat mirrors that of de novo acute myeloid leukemia patients under 60 years who have poor, intermediate and favorable cytogenetics, respectively. 14,15 However, due to low numbers of patients who achieve MMR or deeper response but not MUL and technical limitation in our assay, we cannot accurately determine the relationship between patient survival and MR4 or MR4.5.
In conclusion, a deeper molecular response correlates with a better survival in patients with CML, blast phase. However, achieving CCyR and MMR is inadequate for the optimal survival, and achieving molecularly undetectable leukemia might be the ultimate goal for excellent outcome in patients with CML, blast phase. Our study results could provide a basis for a guideline in the management of CML-BP different from that in the management of CML-CP.