Carfilzomib–lenalidomide–dexamethasone vs lenalidomide–dexamethasone in relapsed multiple myeloma by previous treatment

Carfilzomib, a proteasome inhibitor, is approved as monotherapy and in combination with dexamethasone or lenalidomide–dexamethasone (Rd) for relapsed or refractory multiple myeloma. The approval of carfilzomib–lenalidomide–dexamethasone (KRd) was based on results from the randomized, phase 3 study ASPIRE (NCT01080391), which showed KRd significantly improved progression-free survival (PFS) vs Rd (median 26.3 vs 17.6 months; hazard ratio (HR)=0.690; P=0.0001). This subgroup analysis of ASPIRE evaluated KRd vs Rd by number of previous lines of therapy and previous exposure to bortezomib, thalidomide or lenalidomide. Treatment with KRd led to a 12-month improvement in median PFS vs Rd after first relapse (HR 0.713) and a 9-month improvement after ⩾2 previous lines of therapy (HR 0.720). Treatment with KRd led to an approximate 8-month improvement vs Rd in median PFS in bortezomib-exposed patients (HR 0.699), a 15-month improvement in thalidomide-exposed patients (HR 0.587) and a 5-month improvement in lenalidomide-exposed patients (HR 0.796). Objective response and complete response or better rates were higher with KRd vs Rd, irrespective of previous treatment. KRd had a favorable benefit–risk profile and should be considered an appropriate treatment option for patients with 1 or ⩾2 previous lines of therapy and those previously exposed to bortezomib, thalidomide or lenalidomide.


INTRODUCTION
Important patient-and disease-specific factors, including previous treatments, are considered when selecting a second-or third-line therapy for patients with relapsed multiple myeloma (MM). 1,2 In the United States, bortezomib-and lenalidomide-based regimens are the most commonly used firstand second-line therapies for patients with MM; 3 these regimens also are endorsed by international clinical guidelines. 2,4 As patients progress through multiple lines of bortezomib-and lenalidomide-based regimens in early lines of therapy, they may develop treatment-resistant disease. One study in patients with relapsed MM reported a median duration of response of approximately 10 months in patients with one prior regimen and a median duration of response of approximately 6 months in patients with three prior regimens. 5 Given the prognostic value of the number of previous lines of therapy and the frequent use of bortezomib and lenalidomide as front-line therapy, there is a critical need to evaluate the clinical effect of previous treatment on the efficacy and safety of novel treatment strategies used for patients with relapsed MM.
Carfilzomib is a selective and irreversible epoxyketone proteasome inhibitor that is approved in the United States for use in combination with dexamethasone or lenalidomide plus dexamethasone for the treatment of patients with relapsed or refractory MM (1-3 previous lines of therapy) and as a single agent for the treatment of patients with relapsed or refractory MM (1 or more previous lines of therapy); the agent has also been approved in Europe for use in combination with lenalidomide and dexamethasone (Rd) for the treatment of patients with MM who have received at least one previous line of therapy. 6 The approvals for the combination of carfilzomib, lenalidomide and dexamethasone (KRd) were based on results from a preplanned interim analysis of the randomized, phase 3 ASPIRE trial, which evaluated KRd vs Rd in patients with relapsed MM (1-3 previous lines of therapy). Treatment with KRd led to a significant reduction in the risk of disease progression or death when compared with Rd (hazard ratio (HR) 0.69; 95% confidence interval (95% CI): (0.57, 0.83); P = 0.0001), demonstrating the superiority of KRd vs Rd for the primary endpoint of progression-free survival (PFS). The overall response rate (ORR) 1 was higher in the KRd group vs the Rd group (87.1% (95% CI: 83.4, 90.3) vs 66.7% (95% CI: 61.8, 71.3); odds ratio, 3.47 (95% CI: 2.41, 5.00)), including a complete response (CR) or better in 31.8% and 9.3% of patients in the two groups, respectively. Patients in the KRd group consistently reported superior healthrelated quality of life relative to those in the Rd group during 18 cycles of treatment. 7 As the treatment of patients with relapsed and/or refractory MM who have received multiple lines of therapy is challenging, we performed a subgroup analysis of the interim results from the ASPIRE study to evaluate the efficacy and safety of KRd vs Rd by the number of previous lines of therapy (1 vs ⩾ 2), previous exposure to bortezomib, thalidomide or lenalidomide, and refractory status to bortezomib, thalidomide or lenalidomide in any previous regimen.

Study design and patients
ASPIRE was a randomized, open-label, phase 3 study that evaluated KRd vs Rd in adults with relapsed MM (1-3 previous lines of therapy). Patients previously treated with bortezomib were eligible if they did not experience disease progression during therapy; patients previously treated with lenalidomide were eligible provided they did not discontinue treatment owing to adverse effects, have disease progression during the first 3 months of therapy with Rd, or have progression at any time during treatment if Rd was their most recent treatment. All patients provided written informed consent.
The primary endpoint was PFS in the intent-to-treat (ITT) population. Secondary endpoints included overall survival, ORR, health-related quality of life and safety. Here we present the subgroup analyses of PFS and ORR. Overall survival was not included in this subgroup analysis, as the data were not mature and were awaiting final analysis. The subgroup analysis for PFS and ORR by prior lines, prior exposure and disease resistance were preplanned; however, results are for hypothesisgenerating purposes are not adjusted for multiplicity. Thus, they should be interpreted descriptively. The study protocol was approved by the institutional review boards or ethics committees of all participating sites and was conducted in accordance with the International Conference on Harmonisation Guidelines for Good Clinical Practice and the Declaration of Helsinki. ASPIRE is registered with ClinicalTrials.gov (NCT01080391). Full details regarding the ASPIRE study design have been published. 7 Assessments Disease progression and treatment response were assessed centrally in blinded manner by an independent review committee in accordance with  (14;16) and with deletion 17p in o60% of plasma cells. b Patients must not have progressed during the first 3 months of treatment with prior Rd or progressed at any time if Rd was the most recent line of prior treatment. If a patient progressed during any bortezomib-containing regimen, they were eligible to enroll if the progression date occurred after discontinuation of bortezomib. c Refractory disease was defined as less than a minimal response to, or progression during therapy or within 60 days after completion of therapy.
International Myeloma Working Group Uniform Response Criteria. 8 Minimal response was defined according to European Group for Blood and Marrow Transplant criteria. 9,10 The incidence and severity of adverse events (AEs) were recorded until 30 days after the last dose of study treatment. AEs were graded according to the National Cancer Institute Common Terminology Criteria for Adverse Events (v4.0). 11

Statistical analysis
Efficacy analyses were based on the ITT population. The safety population included all patients who received ⩾ 1 dose of study treatment. Subgroups were defined based on dichotomous baseline variables: previous lines of therapy (1 vs ⩾ 2), previous treatment with bortezomib (yes vs no), previous treatment with thalidomide (yes vs no), previous treatment with lenalidomide (yes vs no), refractory status to any previous bortezomib treatment (yes vs no), refractory status to thalidomide (yes vs no) in any previous regimen and refractory status to lenalidomide any previous regimen (yes vs no). Reported P-values for this subgroup analysis are one-sided, unadjusted for multiple comparisons and are descriptive in nature.

Patient disposition
In total, 792 patients were enrolled and randomized to treatment with KRd (n = 396) or Rd (n = 396), as previously reported. 7 A total of 341 patients had received one prior line of therapy (KRd, n = 184; Rd, n = 157), and 451 patients had received two or more prior lines of therapy (KRd, n = 212; Rd, n = 239). Patients with 1 or ⩾ 2 previous lines of therapy generally had similar baseline characteristics; within these subgroups, baseline characteristics were also generally wellbalanced between the treatment arms (Table 1). However, baseline demographics and disease characteristics may not be relevant at the time of relapse. Median duration of treatment among patients who had received one previous line of therapy was 83.0 weeks in the KRd group and 64.0 weeks in the Rd group; among patients who had received ⩾ 2 previous lines of therapy, median duration of treatment was 90.6 weeks in the KRd group and 52.0 weeks in the Rd group. Other important demographic and clinical characteristics are summarized by previous lines of therapy (   In all, 521 patients (65.8%) had received previous bortezomib (KRd, n = 261; Rd, n = 260); 118 (14.9%) had disease refractory to bortezomib in any previous regimen (KRd, n = 60; Rd, n = 58). In total, 347 (43.8%) had received previous thalidomide (KRd, n = 176, Rd, n = 171); 126 (15.9%) were refractory to thalidomide in any previous regimen (KRd, n = 61; Rd, n = 65) and 157 patients (19.8%) had received previous lenalidomide (KRd, n = 79; Rd, n = 78). A total of 57 patients (7.2%) were refractory to lenalidomide in any previous regimen, 26 (3.3%) were refractory to bortezomib and lenalidomide in any previous regimen and 33 (4.2%) were refractory to bortezomib and thalidomide in any previous regimen.
The cutoff date for the interim analysis was 16 June 2014. 7 Efficacy by line of therapy Consistent with the overall results for the ITT population, the addition of carfilzomib to Rd reduced the risk of disease progression or death in patients who had received 1 previous line of therapy or ⩾ 2 previous lines of therapy (Figures 1a and b Efficacy by type of previous treatment Also consistent with the overall results for the ITT population, treatment with KRd resulted in longer PFS compared with Rd, irrespective of previous exposure to bortezomib, thalidomide or lenalidomide (Figures 2a-f). The median PFS for KRd vs Rd was   Table 4. Notable AEs included hypertension (preferred term; 1 previous line: 11.5% (KRd) vs 4.5% (Rd); ⩾ 2 previous lines: 16.7% (KRd) vs 8.5% (Rd)), acute renal failure (grouped term; 1 previous line: 11.0% (KRd) vs 6.5% (Rd); ⩾ 2 previous lines: 6.2% (KRd) vs 7.7% (Rd)) and dyspnea (preferred term; 1 previous line: 23.1% (KRd) vs 14.3% (Rd); ⩾ 2 previous lines: 16.2% (KRd) vs 15.3% (Rd)).
therapy. Hypokalemia was the only AE of grade ⩾ 3 that occurred ⩾ 5% more frequently with KRd (11.0%) vs Rd (3.4%) in patients who received ⩾ 2 previous lines of therapy. In the KRd group, neutropenia was the only grade ⩾ 3 AE that occurred ⩾ 5% more frequently after ⩾ 2 previous lines of therapy (32.4%) vs 1 previous line of therapy (26.4%). Serious AEs were observed in 63.2% (KRd) and 53.9% (Rd) of patients who received 1 previous line of therapy and 56.7% (KRd) and 53.6% (Rd) of patients who received ⩾ 2 previous lines of therapy. In total, 28.6% of KRd patients and 22.7% of Rd patients who had received 1 previous line of therapy discontinued any study drug owing to an AE; 23.8% of KRd patients and 26.4% of Rd patients who had received ⩾ 2 previous lines of therapy discontinued any study drug for this reason. Nine KRd patients (4.9%) and nine Rd patients (5.8%) who had received one previous line of therapy died while receiving study treatment or within 30 days of the last dose of study drug; 21 KRd patients (10.0%) and 24 Rd patients (10.2%) who had received ⩾ 2 previous lines of therapy died while receiving study treatment.

DISCUSSION
This subgroup analysis of the ASPIRE study indicates that KRd improved outcomes in patients in their first relapse (1 previous line of therapy), patients with ⩾ 2 previous lines of therapy, patients with or without previous exposure to bortezomib, thalidomide or lenalidomide, and in patients refractory to bortezomib or refractory to thalidomide. Given the challenges of treatment in the relapsed and/or refractory setting, where the efficacy of anti-MM agents often declines with successive lines of therapy, 5 it is an important finding that the HRs for PFS consistently favored KRd across all the subgroups based on previous treatment history. Treatment with KRd led to a 12-month improvement in median PFS vs Rd after first relapse and a 9-month improvement in median PFS vs Rd in patients with ⩾ 2 previous lines of therapy, with comparable HRs. Similarly, treatment with KRd led to an approximate 8-month improvement vs Rd in patients with previous exposure to bortezomib, a 3-month improvement in patients with disease refractory to bortezomib, a 15-month improvement in patients with previous thalidomide exposure, an 11-month improvement in patients refractory to thalidomide, a 5-month improvement in patients with previous lenalidomide exposure and a 2-month improvement in patients refractory to lenalidomide. Patients in the KRd group achieved a substantially higher CR or better rate than those in the Rd group, irrespective of the number of previous lines of therapy or the type of previous treatment. In the KRd group, the rate of CR or better was consistent across the number of previous lines of therapy and the type of previous treatment.
As expected, in both treatment groups, absolute median PFS and ORRs were observed to be generally better among patients who had received less previous treatment exposure. In the KRd group, lack of previous exposure to bortezomib, lack of previous exposure to lenalidomide and having one previous line therapy were associated with approximately 6-, 9-and 4month longer median PFS durations compared with those in patients with previous exposure to bortezomib, previous exposure to lenalidomide and ⩾ 2 previous lines of therapy, respectively. In the Rd group, lack of previous exposure to bortezomib, lack of previous exposure to lenalidomide, and having one previous line therapy were associated with approximately 2-, 4-and 1-month longer median PFS durations compared with those in patients with previous exposure to bortezomib, previous exposure to lenalidomide and ⩾ 2 previous lines of therapy, respectively. These findings are consistent with a previous report on the clinical course of patients with relapsed MM, where it was observed that the Effect of prior treatment in the ASPIRE study MA Dimopoulos et al event-free survival rate decreased with each additional line of therapy. 5 It has been hypothesized that the decline in eventfree survival rate with consecutive regimens reflects acquired drug resistance and the presence at relapse of tumor cells with an aggressive phenotype. 5,12 Resistance to the same agent or class of agent may have affected absolute efficacy outcomes in patients previously exposed to bortezomib or lenalidomide in the ASPIRE study. Although carfilzomib has been shown to be effective in patients failing bortezomibbased regimens and to be a more active proteasome inhibitor compared with bortezomib, 13,14 cross-resistance between these two proteasome inhibitors may exist. 12 In the pivotal phase 2 study of single-agent carfilzomib in patients with relapsed and refractory MM, the ORR was lower in patients with ⩾ 2 vs o 2 previous lines of bortezomib-based therapy (18.5% vs 29.5%). 15 KRd reduced the risk of disease progression or death vs Rd across all subgroups analyzed. This benefit was most prominent in patients refractory to thalidomide (HR 0.599; 95% CI: 0.390, 0.918; P = 0.0089) likely due to the relatively short median PFS observed in the Rd arm. The benefit of KRd vs Rd was least evident in patients with disease refractory to bortezomib (HR 0.799; 95% CI: 0.492, 1.297; P = 0.1810), which is expected, given possible cross-resistance between bortezomib and carfilzomib. In addition, patients who were refractory to lenalidomide had only a 2-month improvement in median PFS with KRd vs Rd.
The safety profile of KRd vs Rd in patients with 1 and ⩾ 2 previous lines of therapy was consistent with those reported for the overall population, 7 and no new or clinically significant safety signals were identified. In patients with 1 or ⩾ 2 previous lines of therapy, cardiac and renal AEs were observed at rates consistent with those of previous carfilzomib studies. 6,16 Although patients with MM have been found to develop an increasing number of comorbidities and disease-related complications with additional lines of therapy, 3 the safety profile of KRd in patients with 1 or ⩾ 2 previous lines of therapy was similar.
In addition to carfilzomib, there are many new approved agents for relapsed or refractory MM including ixazomib, elotuzumab and daratumumab. Thus, it will be important for future studies to understand how to best utilize these agents to optimize their benefits in treating patients with relapsed or refractory MM. Future trials evaluating combinations of these agents, including those combining daratumumab and carfilzomib, may also provide important information regarding optimal treatment strategies.
In conclusion, this subgroup analysis of the ASPIRE study indicated that KRd had a favorable benefit-risk profile compared with Rd, irrespective of previous treatment history. KRd should be considered an appropriate treatment option for patients in their first relapse, those who have ⩾ 2 previous lines of therapy, and those previously exposed to and/or refractory to bortezomib, thalidomide or lenalidomide.