Bone marrow stromal cells show distinct gene expression patterns depending on symptomatically involved organs in multiple myeloma

Diagnosis of symptomatic multiple myeloma (MM) is made only when myeloma-related symptoms or end-organ damage occur, including hypercalcemia, renal insuf ﬁ ciency, anemia and bone lesions (CRAB). 1 Little is known at present as to why the clonal proliferation of plasma cells exhibits different organ involvement among myeloma patients, for example, bone lesion vs. renal failure. For the most part, bone disease in myeloma is thought to result from the overexpression of the receptor activator of NF- κ B ligand by bone marrow (BM) stroma, which in turn activates osteoclasts. On the other hand, the pathology of renal disease in myeloma is heterogeneous and may involve a variety of mechanisms. 2 It has been suggested that the amount and biochemical characteristics of light chains are important determi-nants of renal disease presentations in myeloma. 2 The formation of reactive oxygen species has also been suggested as a potential mechanism of light chain-induced renal injury. MM is a unique neoplasm, in that clonal plasma cells coexist among normal hematopoietic cells, with gradual predominant growth and ultimate organ damage caused by deposited immunoglobulin. During this process, BM stromal cells presum-ably contribute to this growth privilege of plasma cells and the determination of which organs will be damaged. 3 At present, few studies have demonstrated the distinct gene expression pro ﬁ les of microenvironmental cells in myeloma compared with normal samples. 4 – 6 On the basis of the hypothesis that BM stromal cells play a major role in the involvement of different organs in myeloma, we compared gene expression pro ﬁ les according to organ involvement after the culture and expansion of BM stromal cells from MM patients with different organ involvement. We cultured BM stromal cells collected from 11 newly diagnosed MM patients (Supplementary

Diagnosis of symptomatic multiple myeloma (MM) is made only when myeloma-related symptoms or end-organ damage occur, including hypercalcemia, renal insufficiency, anemia and bone lesions (CRAB). 1 Little is known at present as to why the clonal proliferation of plasma cells exhibits different organ involvement among myeloma patients, for example, bone lesion vs. renal failure. For the most part, bone disease in myeloma is thought to result from the overexpression of the receptor activator of NF-κB ligand by bone marrow (BM) stroma, which in turn activates osteoclasts. On the other hand, the pathology of renal disease in myeloma is heterogeneous and may involve a variety of mechanisms. 2 It has been suggested that the amount and biochemical characteristics of light chains are important determinants of renal disease presentations in myeloma. 2 The formation of reactive oxygen species has also been suggested as a potential mechanism of light chain-induced renal injury.
MM is a unique neoplasm, in that clonal plasma cells coexist among normal hematopoietic cells, with gradual predominant growth and ultimate organ damage caused by deposited immunoglobulin. During this process, BM stromal cells presumably contribute to this growth privilege of plasma cells and the determination of which organs will be damaged. 3 At present, few studies have demonstrated the distinct gene expression profiles of microenvironmental cells in myeloma compared with normal samples. [4][5][6] On the basis of the hypothesis that BM stromal cells play a major role in the involvement of different organs in myeloma, we compared gene expression profiles according to organ involvement after the culture and expansion of BM stromal cells from MM patients with different organ involvement.
We cultured BM stromal cells collected from 11 newly diagnosed MM patients (Supplementary Table S1). One patient presented as asymptomatic MM, and the remaining 10 MM patients were classified according to their myeloma-defining events (MDEs). Bone lesions were mainly detected by complete skeletal survey, and renal function was assessed by serial measurement of creatinine levels (detailed methods are described in Supplementary Information). Six patients presented with multiple lytic bone lesions (bone lesion group): five patients without renal failure and one patient (Patient 3) with transient renal impairment. Three patients presented with renal failure with creatinine clearance o30 ml min − 1 at diagnosis and o20 ml min − 1 at the end of follow-up (renal failure group) in the absence of bone disease, and one patient presented with anemia (hemoglobin 8.9 g dl − 1 ) without bone lesions and without renal failure (anemia group). For all 5 patients without bone lesions, X2 imaging analyses were performed, either by different methods in addition to complete skeletal survey (Patient 10, computed tomography; and Patient 11, magnetic resonance imaging) or with X2 skeletal surveys at different times (Patients 1, 8 and 9, at diagnosis and at disease progression).
In addition, we cultured BM stromal cells from patients with other plasma cell neoplasms (Supplementary Table S2). One patient (Patient 12) had two bone plasmacytomas in the spine and femur without other symptoms and o 10% plasma cells in BM (plasmacytoma group). Two patients were diagnosed with AL amyloidosis (one with cardiac amyloidosis and the other with renal amyloidosis), and one patient was diagnosed with POEMS syndrome. The control groups were composed of nine B-cell lymphoma patients with no evidence of BM involvement and four patients with mild-to-moderate cytopenia without evidence of hematologic malignancies.
Cultured BM stromal cells were analyzed after passage five for all patients. The passages at analysis and growth rates of BM stromal cells varied among patients; however, there were no differences between disease and control groups (Supplementary Table S3). Flow cytometric analysis of BM stromal cells from the MM (n = 4) and control patients (n = 5) did not indicate the expression of hematopoietic lineage antigens but did indicate the positive expression of CD90, CD105 and CD44. Cytogenetic studies using G-banding and interphase fluorescence in situ hybridization (FISH) to detect common chromosomal abnormalities known to frequently occur in MM was performed on patients' BM aspiration specimens and cultured BM stromal cells (Supplementary Table S4). Eight patients exhibited FISH abnormalities in BM plasma cells, including IGH translocations (n = 6), RB1 deletions (n = 5), 1q duplications (n = 5) and trisomies (n = 3). IGH translocations were detected in 3/6 patients with bone lesions (50%) and in 2/3 patients with renal failure (67%). We tested for BM stromal cells from 10 MM patients using the same FISH probes; however, none of the patients presented the cytogenetic abnormalities observed in the corresponding malignant plasma cells.
When the global gene expression profiles of BM stromal cells from 11 MM patients were compared with those of stromal cells from the 13 control patients, the patient and control groups did not form clearly separated clusters. Otherwise, the patient-derived BM stromal cells exhibited preferential grouping into several main clusters according to clinical manifestations. The gene expression profiles of BM stromal cells from patients with multiple lytic bone lesions and those from patients with renal failure presented as different clusters (Figure 1a). Among the differentially expressed genes in BM stromal cells from MM patients with multiple bone lesions, vascular cell adhesion molecule 1 (VCAM1) was overexpressed 3.27-fold. In addition, extracellular matrix genes, such as collagen type IV (COL4A4), periostin (POSTIN), fibulins (FBLN2 and FBLN5) and secreted frizzled-related protein 4 (SFRP4), were overexpressed (Table 1). Differentially expressed genes in BM stromal cells from MM patients with renal failure were associated with cell signaling (RGS17, STEAP1). The qPCR results presented higher relative expression levels for the VCAM1 gene in MM with bone lesions (Figure 1b). In the gene set enrichment analysis, the enriched gene sets of BM stromal cells from MM patients with bone lesions were involved in the cellular regions and biological processes of the extracellular matrix (Figure 1c). MM patients with renal failure presented with enhanced expression of gene sets associated with G-protein coupled receptor signaling and the biological process of transmembrane transporter activity (Figure 1d).
In previous studies, distinct gene expression profiles in MM BM mesenchymal stem cells (MSCs) has been reported. 4 In a previous study, 4140 genes were differentially expressed between MM    An interesting result of this study is that the highly expressed genes and gene sets present plausible relationships between the MDEs of symptomatic MM and other plasma cell neoplasms. First, MM patients with bone lesions showed elevated expression levels of extracellular matrix-associated genes, such as COL4A4, POSTN, FBLN2, FBLN5, SFRP4 and VCAM1. VCAM1 is an adhesion molecule that binds with high affinity to integrin α4β1 and is constitutively expressed in BM stromal cells. 7 The binding of myeloma cells to BM-MSCs through α4β1-integrin and VCAM1 interaction induces the decreased secretion of osteoprotegerin and the increased expression of receptor activator of NF-κB ligand, which promotes osteolysis. 8,9 The profiles of myeloma with bone lesions were rather homogenous, whereas those with renal failure were heterogeneous. This finding is consistent with past observations that myeloma with renal involvement shows a heterogeneous pathology involving a variety of mechanisms. 2,10 When the results of other plasma cell neoplasms were analyzed, notch 2 N-terminal-like (NOTCH2NL) expression was high in patients with plasmacytoma (Supplementary Figure S1). In myeloma cells, NOTCH signaling is thouhgt to be involved in BM homing, MM cell metastasis, and matrix invasion. 11 In a patient with POEMS syndrome, stathmin-like 2 (STMN2), which encodes the microtubule regulatory protein stathmin, was found to be overexpressed. Interestingly, stathmin is reported to be associated with the expression of VEGF and hypoxia-inducible factor a (HIF-1a). 12 VEGF is well known as an important cytokine in the pathogenesis of POEMS syndrome. 13 In AL amyloidosis, the expression of the lambda light chain (IGLL1) gene was significantly elevated; however, we could not confirm this result via qPCR (Supplementary Figure S1). Although these results might offer interesting clues, with data from one or two patients, it was not possible to determine whether the genetic variations were disease subtype-specific or patient-specific. More patients with POEMS syndrome or AL amyloidosis should be included.
The correlation between cytogenetic abnormalities in plasma cells and MDEs of MM patients has been reported. 1 In the present study, we found no specific correlation between chromosomal abnormalities and MDEs. Considering our results of varying gene expression patterns in BM stromal cells among MM patients with different MDEs, we suggest that the microenvironment, in addition to the malignant plasma cells themselves, is also an important factor in determining the clinical symptoms of MM patients.
The small number of patients used is a limitation of this study; therefore, it is difficult to draw definitive conclusions. To confirm the role of stromal cells in determining organ damage, data from a larger study population that encompasses different ethnic groups, as well as in vitro and in vivo functional studies, will be needed. In addition, the expression profiles were investigated beyond the fifth passage of BM stromal cells; therefore, in vitro culture conditions could have affected the gene expression characteristics of the BM stromal cells. However, these results may provide novel information that was absent from previous studies that investigated BM stromal cells at very early passages. For some patients, only complete radiologic skeletal surveys were performed to detect bone lesions. Approximately 10-20% of bone lesions are known to be missed by conventional radiology. 14 Therefore, with the use of more sensitive techniques, bone lesions may be detected in more patients. Notably, as described above, for the Letter to the Editor three patients without bone lesions, two complete skeletal surveys were performed at diagnosis and at disease progression. Repeated testing may increase sensitivity compared conventional radiologic bone survey alone, thereby reducing the likelihood that bone lesions might be missed.
In conclusion, the gene expression profiles of BM stromal cells in MM patients differed among patients with different organ involvement, and we hypothesize that differentially expressed genes in stromal cells could play important roles in symptomatic myeloma. Considering that different patients with the same disease could have variable clinical manifestations, organ involvement and disease course, our observations provide an alternative explanation for individual patient variability other than tumor cell heterogeneity.