Table 3 Correlation of selected mutations in RTKs and adhesion molecules with gene expression, CN changes and copy neutral LOH

From: Multiple myeloma is affected by multiple and heterogeneous somatic mutations in adhesion- and receptor tyrosine kinase signaling molecules

Gene GE CN LOH CL Predictors
(A)
IGF1R +   L363 3
NTRK2   L363 3
NTRK1 +   MM1S 2
EGFR   AMO1 2
EGFR   MM1S 2
ERBB3 +   AMO1 3
ERBB3   L363 3
(B)
SDK1 +   JJN3 3
LRFN5 X MM1S 3
LRFN5   U266 3
DCHS2 +   OPM2 3
DCHS2   MM1S 3
SIGLEC1 +   U266 2
SIGLEC1   MM1S 3
PCDHGA7   JJN3 2
PCDH10   AMO1 3
THBS1   AMO1 2
BCAN ++ X AMO1 3
BCAN ++   OPM2 3
LAMB2 ↓↓↓ X MM1S 3
CAMTA1   OPM2 3
ADAMTS2   OPM2 3
ADAMTS2   AMO1 2
LAMA5 ↓↓ +   OPM2 2
LAMA5   MM1S 3
TNC ↑↑   AMO1 3
ADAMTS19   U266 3
COL12A1   L363 3
CDH11   L363 3
CELSR2 +   JJN3 3
ITGB1 ↓↓ X U266 3
PCDH20 +   AMO1 3
RELN ↓↓↓ +   U266 3
  1. Abbreviations: CL, cell line; CN, copy number; GE, gene expression; LOH, loss of heterozygosity; MM, multiple myeloma; RTK, receptor tyrosine kinase.
  2. One arrow represents a twofold up- or downregulation, one and a half arrows a threefold up- or downregulation, two arrows a fourfold up- or downregulation, and three arrows a ninefold up- or downregulation in mRNA expression compared with the median mRNA expression of all six cell lines; ± represents a simple copy number gain (three copies) and ++ represents four copies of that gene. Copy neutral LOH or LOH that was accompanied by a chromosomal gain is depicted by x.
  3. aRTKs and adhesion molecules that were mutated in at least 1 of the 43 primary MM (including Chapman et al.5) and 1 cell line, that were ‘damaging’ by at least 2 functional predictors and that were validated by Sanger sequencing. These conditions apply to all genes of Table A and B.