Conjugated polymer nanomaterials (CPNs), as optically and electronically active materials, hold promise for biomedical imaging and drug delivery applications. This review highlights the recent advances in the utilization of CPNs in theranostics. Specifically, CPN-based in vivo imaging techniques, including near-infrared (NIR) imaging, two-photon (TP) imaging, photoacoustic (PA) imaging, and multimodal (MM) imaging, are introduced. Then, CPN-based photodynamic therapy (PDT) and photothermal therapy (PTT) are surveyed. A variety of stimuli-responsive CPN systems for drug delivery are also summarized, and the promising trends and translational challenges are discussed.
Conjugated polymers (CPs) are a special class of macromolecules with large π-conjugated backbones. Owing to their highly electron-delocalized structures and efficient coupling between optoelectronic segments, CPs have a particular capability to absorb and emit light energy, which can be effectively converted to fluorescence, heat, and other energies1,2,3,4. Conjugated polymer nanomaterials5,6,7,8,9,10,11 (CPNs), as both optically and electronically active materials, have been shown to be promising theranostic agents12,13,14,15,16,17,18. Owing to their excellent light-harvesting and light-amplifying properties, CPNs have been applied to both in vitro and in vivo fluorescence imaging to achieve real-time diagnostics19,20,21,22,23,24. However, conventional fluorescence imaging suffers from general limitations such as poor spatial resolution and shallow tissue penetration25,26,27. Recently, with advancements in optical imaging techniques and materials science, a variety of powerful in vivo imaging methodologies have emerged28. Among them, near-infrared (NIR) imaging29,30, two-photon (TP) imaging31,32, photoacoustic (PA) imaging27,33, and multimodal (MM) imaging34,35,36 can provide deep tissue penetration and high spatial resolution, which offer new opportunities for the innovative application of CPNs in theranostics.
In addition to directly providing diagnostic signals, CPNs are also involved in minimally invasive therapeutic techniques such as photodynamic therapy (PDT)37,38,39,40 and photothermal therapy (PTT)41,42,43,44. PDT is an effective noninvasive therapeutic technique used in clinical cancer treatment. This technique utilizes photosensitizers to transfer energy from light to oxygen molecules and generate cytotoxic reactive oxygen species (ROS). By using light-absorbing agents, PTT converts the light radiation into thermal (vibrational) energy to ablate cancer cells. PTT has emerged as a next generation of noninvasive methodology for cancer therapy. In addition, the convergence of drug delivery systems with PDT or PTT for combination therapy has also been achieved for enhanced therapeutic efficacy45,46,47,48,49. In this review, we will focus on CPN-based theranostic (a combination of diagnostics and therapy) systems for imaging, PDT and PTT, as well as stimuli-responsive drug delivery (Figure 1). Future opportunities and challenges will also be discussed.
CPNs for biomedical imaging
Fluorescence microscopy techniques play a crucial role in biomedical imaging50. Owing to their highly efficient light harvesting, multiple emissive wavelengths, ease of modification, excellent photostability and low cytotoxicity, CPNs have recently attracted considerable attention in biological fluorescence imaging and sensing18,51,52. Most current optical theranostic techniques rely on emitted photons as the signal readout and thus inevitably suffer from the drawbacks of autofluorescence background of the tissue and limited capability of light penetration, which may compromise the diagnostic accuracy25,27. To meet the requirement of in vivo applications, a variety of new CPs and relevant imaging techniques, including NIR imaging, TP imaging, PA imaging and MM imaging, have been exploited.
Fluorescence imaging in the second near-infrared (NIR II, 1.0–1.7 μm) region has recently attracted significant attention owing to the advantages in imaging in the visible (400–750 nm) and the conventional near-infrared (NIR 1750–900 nm) regions53,54,55. Photons in the NIR-II region can provide high spatial resolution at deep tissue penetration depths owing to the reduced scattering of long-wavelength photons56. For example, Hong and co-workers synthesized a series of the CPs with tunable emission wavelengths in the NIR-II region through donor-acceptor alternating copolymerization57 (Figure 2A). They functionalized the polymer core non-covalently with a PEGylated surfactant as the shell, which afforded water solubility and biocompatibility (Figure 2B and 2C). The NIR-II fluorescent probe exhibited a quantum yield of 1.7% and thus allowed for in vivo and deep-tissue imaging. Ultrafast imaging of mouse arterial blood flow with an unprecedented imaging speed of more than 25 frames per second was achieved (Figure 2D and 2E). The fluorophores improved the penetration depth and enabled dynamic imaging with great spatiotemporal resolution, thus demonstrating that NIR-II fluorescence systems are suitable for clinical applications. Most recently, the same group developed a novel NIR-II fluorescent probe to investigate cerebrovascular injury in a mouse traumatic brain injury (TBI) model58. With the aid of an in vivo NIR-II imaging system, they could directly visualize dynamic vascular changes in a mouse TBI model, including initial transient hypoperfusion that was resolved as fluorophore leakage and accumulation caused by damage to the cerebrovasculature.
TP excitation microscopy is a noninvasive imaging technology for living cells and tissues with excellent spatial resolution, deep-tissue penetration, less interference from autofluorescence, and minimal photodamage to living bio-substrates31,59,60. Distinguished from the conventional one-photon excitation microscopy, TP excitation arises from the simultaneous absorption of two photons in a single quantitized event. Thus, the large two-photon absorption (TPA) cross sections and high emission quantum yields of fluorophores are the key requirements for TP imaging61,62. CPs have highly delocalized π-conjugated backbones and display large one- and two-photon absorption coefficients, high fluorescence quantum yields, and good photostability, which make them promising for TP imaging63,64. In addition, the wavelength of excitation light for TP imaging is usually in the NIR regions (700–1000 nm), where water and blood are nearly transparent and non-scattering65,66.
Li and co-workers reported the use of red-emitting dye-doped CP nanoparticles for TP cancer cell imaging67. Conjugated polymer (PFV) and red-emitting dye magnesium phthalocyanine (MgPc) were encapsulated in polyoxyethylene nonylphenylether (CO-520) to form nanoparticles with the excitation wavelength of 800 nm (Figure 3A). In these nanoparticles, PFV with large TPA cross sections was chosen as a TP light-harvesting material, and MgPc with high emission quantum yields was chosen as the energy acceptor and red-emitting material (Figure 3B). The TP-based fluorescence resonance energy transfer (FRET) from the CPs to the dyes could be utilized to prepare NIR excited red-emitting materials for deep-tissue live-cell imaging; it can be applied to most CPs with light absorption in the visible range. The TP excitation fluorescence of MgPc in MgPc/PFV NPs was enhanced by up to 53 times. These nanoparticles displayed excellent biocompatibility (Figure 3C). In the TP imaging of HepG2 cancer cells, the cytoplasm could be clearly distinguished. Additionally, the cell morphology was readily discerned by the strong fluorescence (Figure 3D).
PA tomography is an emerging technology that overcomes the multiple scattering of optical photons in biological tissues by making use of the photoacoustic effect. Light absorption by photoacoustic contrast agents creates a thermally induced pressure jump that launches ultrasonic waves, which are received by acoustic detectors to form images27. PA imaging exceeds the optical diffusion limit via detection of phonons, instead of photons, upon light excitation68,69,70. PA imaging has great potential for the visualization of physiology and pathology at the molecular level because of the deep tissue penetration and excellent spatial resolution. Pu and co-workers reported NIR-absorbing semiconducting polymer nanoparticles (SPNs) as an efficient and stable nanoplatform to generate ultrasound wave for in vivo photoacoustic molecular imaging71. Two polymer derivatives with strong absorption within the NIR region were chosen (Figure 4A–4C). These nanoparticles produced a stronger signal than the commonly used single-walled carbon nanotubes and gold nanorods, permitting whole-body lymph node photoacoustic mapping in living mice with low-volume injections (Figure 4D). Furthermore, they coupled the nanoparticles to a cyanine dye derivative (IR775S) that was sensitive to ROS-mediated oxidation to obtain a probe for ROS imaging. This example of ROS imaging demonstrated that the conjugated polymer nanoparticles possessed high structural flexibility and could be an ideal nanoplatform for photoacoustic molecular probes. The PA signal comes mainly from photothermal conversion. Thereby, the criterion for selecting PA imaging probes is consistent with that for PTT. This similarity makes PA and PTT an ideal pair to be seamlessly and synergistically combined into theranostics. Lyu and co-workers further confirmed that the PA intensity of SPNs was proportional to their photothermal conversion efficiency and thus envisioned the utility of SPNs in cancer treatment72. Recently, Fan et al demonstrated perylene-diimide-based nanoparticles as highly efficient photoacoustic agents for deep brain tumor imaging73.
Compared to single modality, MM imaging can satisfy the increasing requirements in advanced biotechnology. Sun and co-workers developed conjugated polymer-labeled magnetic nanoparticles using a layer-by-layer assembly technique for dual-modal fluorescent-magnetic resonance imaging74,75 (Figure 5A and 5B). The fluorescent-magnetic nanoparticles were used for both tracking the cellular uptake of nanomaterials and controlling the cellular uptake by an external magnetic field. The results demonstrated a higher uptake ability of the fluorescence-labeled magnetic nanoparticles with the aid of a magnetic field. Subsequently, Li and co-workers synthesized the conjugated polymer with emissions in the far-red/near-infrared region20. They fabricated the fluorescent magnetic nanoparticles by co-encapsulating the conjugated polymer and lipid-coated iron oxides (IOs) with a mixture of poly(ethylene glycol)-folate and PLGA for in vivo MM imaging (Figure 5C and 5D).
Liu et al reported a fluorescence-Raman dual-imaging method for intercellular tracking76. They fabricated a CPE-cMWNT nanosystem with electrostatic adsorption between oppositely charged carbon nanotubes (cMWNTs) and conjugated polyelectrolytes (CPEs) (Figure 6). The red fluorescent cationic CPE (TBtPFN) was coated on the negatively charged surface of cMWNTs to form TBtPFN/cMWNT nanocomposites and these were then coated with green fluorescent anionic CPE (BtPFS) as a second layer to form the dual-color fluorescence-labeled carbon nanotubes (BtPFS/TBtPFN/cMWNTs). Fluorescence-Raman dual-imaging showed the characteristic red and green fluorescence of TBtPFN and BtPFS, respectively. Meanwhile, carbon nanotubes produced robust and resonance-enhanced Raman bands owing to their diameter and electronic structure, which could be used for Raman mapping of CPE-cMWNT nanocomposites distribution in Bel-7402 cells. The high flexibility of electrostatic assembly facilitates their interaction with various biological substances for drug delivery and diagnostic imaging.
Fan and co-workers reported a novel melanin-based MM imaging nanoplatform for PA imaging, positron emission tomography (PET) and magnetic resonance imaging (MRI)77 (Figure 7). Melanin is a naturally occurring pigment, and has been pursued as a biomarker for melanoma imaging78,79. The authors prepared ultra-small melanin nanoparticles (MNPs) from melanin granules and further used these water-soluble MNPs as a good endogenous nanoplatform for tumor multimodality imaging. The MNPs showed unique the photoacoustic property and natural binding ability with metal ions (such as 64Cu2+, Fe3+) (Figure 7A). In vivo imaging results showed that MNPs not only serve as a photoacoustic contrast agent for PA imaging but also actively chelate metal ions for PET and MRI (Figure 7B–7E). Recently, the same group developed an MNP-based drug delivery system for multimodality-imaging guided chemotherapy80. This work provided an efficient single nanosystem for tumor therapy and clinical translation.
In addition, CPN-based MM imaging systems as biosensing fluorescent probes have attracted considerable attention in recent years. For example, Wang and co-workers developed an optical nanoruler system for label-free protein detection based on the distance-dependent metal-enhanced fluorescence (MEF) effect81. The detection mechanism is shown in Figure 8. A water-soluble cationic conjugated polymer (PFVCN) was selected as the fluorescence probe. Quartz slides with silver prisms nanostructures were used to produce the MEF effect. After bioconjugation of the antibody onto the surface and the antibody-antigen recognition, the distance between surface-bound PFVCN and bottom Ag nanostructure changes, leading to the MEF effect. Thus, changes in the fluorescent signal of PFVCN represented the binding event of the specific protein. Most recently, the same group constructed a hybrid probe of graphene oxide and cationic conjugated polymer for calmodulin sensing based on a fluorescence resonance energy transfer (FRET) strategy82. The presence of graphene oxide played an important role in sensing calcium ions by FRET between the conjugated polymer and calmodulin.
CPNs for phototherapy
In addition to biological imaging, CPNs have also been explored as agents for phototherapy17,83. CPNs can sensitize oxygen molecules under light irradiation to produce ROS and thus be used in PDT84,85,86. Moreover, CPNs with strong NIR absorption and high heat conversion efficiency have potential as a new generation of agents for PTT87. PDT and PTT hold great promise for non-invasive cancer treatment, and CPN-based image-guided therapy is a burgeoning area of research87,88,89,90,91,92,93. In addition, several new therapeutic approaches based on the mechanism of PDT and PTT have also been investigated. We will introduce the most recent advances in this section.
Most of the CPs with short-lifetime fluorescent emission suffer interference from background fluorescence18. Thus, developing novel CPNs with high utilization efficiency remains challenging. Shi and co-workers developed novel phosphorescent conjugated polymer dots (Pdots) containing Ir(III) complexes for optical oxygen sensing and photodynamic cancer therapy23. The long emission lifetime of the phosphorescent Ir(III) complexes can offer an effective way to eliminate the interferences from autofluorescence94,95. The Pdots were composed of polyfluorene units and phosphorescent Ir(III) complexes in the main polymer chains via Suzuki coupling reaction and offered an efficient energy transfer from the conjugated polymer main chain to the phosphorescent Ir(III) complex (Figure 9A). The Pdots possessed fine photostability, biocompatibility, and ultrasmall size (<10 nm) (Figure 9B), which made the cellular uptake of the Pdots easier. The Pdots with phosphorescent Ir(III) complexes could serve as an optical probe for monitoring oxygen due to the triplet state that can quench the triplet phosphorescence. Meanwhile, the energy transfer enables the 1O2 generation, causing effective apoptosis and death of cancer cells in the photodynamic therapy process (Figure 9C). This study provided a strategy for designing multifunctional nanoplatforms in tumor hypoxia sensing, imaging, and PDT applications24,86,96,97,98,99,100.
Lyu et al found that NIR-absorbing semiconducting polymer nanoparticles (SPNs) with high photothermal conversion efficiency could control the thermo-sensitive ion channels in neurons101 (Figure 10). The SPNs absorbed NIR laser at 808 nm with deep tissue penetration capability. Transient receptor potential cation channel subfamily V member 1 (TRPV1) is a protein widely expressed in the mammalian nervous system102. The surface of SPNs were functionalized with the anti-TRPV1 antibody, which targeted the temperature-sensitive ion channels (Figure 10A and 10B). When exposed to NIR laser irradiation at 808 nm, the SPNs remotely increased the local temperature, then rapidly and specifically activated the TRPV1 to gate the intracellular calcium influx of the neural cells in a reversible and safe manner (Figure 10C). The ability of the nanoparticle to activate TRPV1 was evaluated using mouse neuroblastoma/rat DRG neuron hybrid ND7/23 cells. This CPN-based photothermal approach activated neurons within milliseconds.
CPNs for stimuli-responsive drug delivery systems
Stimuli-responsive drug delivery systems (DDSs) are promising for treating a variety of diseases through improvements in biological specificity and therapeutic efficacy103,104,105,106,107,108,109,110,111,112,113. CPN-based DDSs have been devoted to creating an on-demand drug release system via different triggers, including electrostatic repulsion, pH, enzyme, ATP, ROS, temperature, and hypoxia10,11. Furthermore, CPNs provide an excellent opportunity to integrate imaging and therapeutic capability into a single delivery system, facilitating the real-time monitoring of localization of the drugs and assessment of in vivo therapeutic efficacy.
Yu and co-workers developed a cationic conjugated polymer core-shell nanoparticle for delivery of short interfering RNA (siRNA) and electrostatic repulsion-triggered intracellular release of the cargo114. The designed nanocarrier had three components: a cationic yellow-emissive conjugated polymer (BtPFN) nanoparticle as the core, an anionic siRNA attached to the core, and a sequentially electrostatically adsorbed layer of a cationic green-emissive conjugated polymer (ThPFN) as the shell (Figure 11). The cationic ThPFN shell protected the delivered siRNA from ribonuclease and interacted with the negatively charged cell membranes, leading to high transfection efficiency. Owing to the large overlap in the spectra of ThPFN emission and BtPFN absorption, FRET occurred within the three-layer complexes. After uptake by the cells, siRNA was gradually released from the complexes, which was attributed to the protonation of the base groups of siRNA in acidic endosomes resulting in weakened electrostatic interactions between siRNA and the cationic polymer115,116,117.
A CPN-based nanoplatform also has the potential to become a theranostic tool for imaging-guided therapy in the personalized treatment of some malignancies. Liu and co-workers also reported on CPN-based theranostic nanoplatforms (NIR NPs) for the effective delivery of siRNA in vivo and simultaneous real-time tracking of tumor accumulation via noninvasive NIR imaging118 (Figure 12A). In this unique electrostatic assembly strategy114, cationic lipid is added to afford the nanomaterials. The NIR NPs were small (∼50 nm) (Figure 12B), displayed long blood circulation and high tumor accumulation, and facilitated tumor in vivo imaging (Figure 12C). In addition, the NIR NPs were also used for NIR imaging of sentinel lymph nodes (SLNs), which indicated that the NPs may be useful in imaging-guided location and removal of lymph nodes (Figure 12D). Immunochemical histological observation showed that the NIR NPs can efficiently silence the expression of V-Raf murine sarcoma viral oncogene homolog B (BRAF) in tumor tissues (Figure 12E). Furthermore, in vivo results revealed that this siRNA delivery system provided significant inhibition of tumor growth and metastasis in an orthotopic mouse model of anaplastic thyroid cancer (ATC) (Figure 12F).
Yu et al described a pH-responsive and NIR-emissive CPN for the simultaneous delivery and tracking of the anticancer drug doxorubicin (DOX)119. They utilized the CPNs to monitor the release of the anticancer drug in a non-invasive and real-time manner. In vivo studies showed that during degradation of the nanoparticles within a mildly acidic microenvironment at the tumor site, the NIR fluorescence intensity of the conjugated polymer decreased remarkably with the fading of FRET efficiency between the CPN and DOX. Meanwhile, the growth of the tumor was significantly inhibited. The dual-functional nanoparticles facilitated cancer therapy by monitoring drug biodistribution in vivo.
Inspired by the “closed-loop” design120,121, Chen et al came up with the “negative-feedback” control circuit to treat obesity122. Lipase is the principal enzyme responsible for hydrolysis and subsequent absorption of dietary fat123. They designed a lipase-sensitive conjugated polymer-based nanocarrier encapsulating orlistat, a pancreatic and gastro-intestinal lipase inhibitor124 (Figure 13A). The nanocarriers were subject to degradation in the digestive tract because the high concentration of lipase triggered the release of the loaded orlistat. The released drug irreversibly deactivated the lipase, which in turn decreased the release speed of the drug payload, creating a negative-feedback circuit (Figure 13B). Meanwhile, orlistat reduced the gastro-intestinal absorption of fats, which finally led to a long-term reduction of body weight. Diet-induced obesity (DIO) mice were then treated by CPNs through oral gavage. The results revealed that a single dose of the nanocarrier prevented weight gain over eight days. Compared to the control group, the daily administration of the CPNs led to the lower weight of livers or fat pads, lower total cholesterol level, and smaller adipocyte size.
Qian and co-workers reported an innovative conjugated polymer-based nanocarrier capable of achieving NIR imaging and adenosine-5′-triphosphate (ATP)-responsive anticancer drug release125. Considering the distinct difference in the ATP levels between the extra- and intra-cellular milieu, CPNs were functionalized with phenylboronic acid on the surface as binding sites, which could be converted to the water-soluble polyelectrolytes in an ATP-rich environment. In vivo studies showed that this formulation exhibited promising capability for inhibiting tumor growth. Furthermore, the metabolism process could be evaluated by monitoring the fluorescence signal of the conjugated polymer via in vivo NIR imaging.
Liu and co-workers reported a ROS-activatable polyprodrug, which conjugates the DOX molecules to a polyelectrolyte via ROS-cleavable dithioketal linkers46. This polyprodrug system can be utilized for targeted and image-guided on-demand PDT and chemotherapy (Figure 14). To obtain biocompatibility and targetability, they also conjugated hydrophilic PEG and cyclic arginine-glycine-aspartic acid tripeptide (cRGD) to the conjugated polymer (Figure 14A). In aqueous media, the obtained polyprodrug self-assembled into nanoparticles (NPs), which exhibited both bright fluorescence for cell imaging and served as a photosensitizer to generate ROS efficiently for triggering on-demand DOX release and PDT (Figure 14B). Cell imaging and flow cytometry showed the effective light-controlled drug release. In vitro cytotoxicity studies confirmed enhanced cell viability inhibition for the combined therapy compared to single modality treatment.
Liu and co-workers also reported a photothermal-activatable CP nanoplatform for cancer therapy92. Upon a single laser irradiation, the conjugated polymer could efficiently convert the laser energy into hyperthermal energy for PTT. Moreover, the hydrophobic polymer matrix bearing many 2-diazo-1,2-naphthoquinones (DNQ) moieties could be transformed into a hydrophilic one simultaneously, leading to rapid drug release. The synchronous combination of PTT with chemotherapy showed a synergistic therapeutic efficiency compared to single modality treatment.
Recently, Qian et al designed a conjugated polymer-based nanocarrier for enhanced anticancer therapy by integrating a PDT system with a hypoxia-responsive drug delivery system48. This system was capable of light-induced 1O2 generation and hypoxia-responsive drug release. The conjugated polymer (CP-Br) with a dithiophene-benzotriazole126 moiety could be utilized as a visible/near-infrared light activated ROS generation source. Then, the CP-Br was further grafted with a hydrophobic hypoxia-sensitive molecule, 2-nitroimidazole (NI), to achieve hypoxia-responsive transduction. NI could be converted to hydrophilic 2-aminoimidazoles under hypoxia via a single-electron reduction catalyzed by a series of nitroreductases coupled to bio-reducing agents, such as NADPH, a plentiful coenzyme in tissues127. The synthesized ROS-generating and hypoxia-sensitive conjugated polymer (CP-NI) was a hydrophobic polymer. A double-emulsion-based solvent evaporation/extraction method was utilized to encapsulate DOX and form a nanoscale hypoxia-responsive vesicle (Figure 15A). When the nanocarriers accumulated at the tumor microenvironment and were irradiated, the CP-NI produced singlet oxygen (1O2), resulting in the disruption of the endo-/lysosomal membrane and the induction of cancer cell apoptosis128. Simultaneously, the dissolved oxygen could be rapidly consumed, resulting in a local hypoxic environment129. Subsequently, NI groups on the CP-NI were reduced to hydrophilic 2-aminoimidazoles under bioreductive conditions in cells, promoting the dissociation of DOX/CP-NI, and the release of cargo. The released DOX accumulated in the cell nuclei and induced DNA damage-mediated cytotoxicity130 (Figure 15B). In vitro results revealed that the delivery system significantly enhanced cell cytotoxicity and apoptotic activity. Furthermore, in vivo experiments demonstrated that this delivery system provided more significant tumor growth inhibition compared to the PDT alone.
With a host of unique properties, including highly efficient light harvesting and emitting, excellent photostability, relatively low cytotoxicity, and versatile surface modification, conjugated polymer nanomaterials have exhibited tremendous potential for diagnostics and/or therapeutic applications. Despite the advancements described in this review, there are still many challenges to be addressed. First, the quantum yield of NIR-emissive CPNs is not as high as other typical fluorescent imaging probes131. Therefore, there is still a great need to overcome the current limitations and explore more potential applications. Additionally, with the unique optoelectronic characteristics, CPNs have considerable potential in the development of high-resolution fluorescence imaging technology. Second, considering the π-conjugated backbone, the metabolic processes of CPNs are not yet clear. The systemic toxicities of CPNs also require significant assessment. Third, the light-induced charge transfer could interact with other biological activities and requires further detailed study. The outcomes of these studies would further broaden the applications of CPNs in photosynthesis, optogenetics, and the transmission of neural signals.
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This work was supported by the National Natural Science Foundation of China (No 21174060) to Qun-dong SHEN, the Sloan Research Fellowship to Zhen GU, and Program A for Outstanding PhD candidate of Nanjing University to Cheng-gen QIAN. The authors also thank the China Scholarship Council for financially supporting Cheng-gen QIAN's research and living abroad.
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Qian, Cg., Chen, Yl., Feng, Pj. et al. Conjugated polymer nanomaterials for theranostics. Acta Pharmacol Sin 38, 764–781 (2017). https://doi.org/10.1038/aps.2017.42
- conjugated polymer
- biomedical imaging
- photodynamic therapy
- photothermal therapy
- stimuli-responsive drug delivery
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