Fasudil shifts M1 to the M2 polarization of BV-2 microglia. The BV-2 cells were cultured in the absence or presence of IFN-γ (100 U/mL) and/or fasudil (15 μg/mL) for 72 h. The protein was extracted from the BV-2 microglia, and the expression of iNOS and arginase was measured using Western blotting (A). The production of NO and the activity of arginase were detected by chemical and enzymatic assays (B). Simultaneously, the levels of TNF-α and IL-10 were detected using ELISA kits (C). The results of the Western blot analysis were expressed as the fold change relative to GAPDH as the loading control, and the levels of cytokines were expressed in pg/mL. The quantitative analysis was performed based on 3 independent experiments with similar results. bP<0.05, cP<0.01.