Abstract
Aim:
To screen for interferon (IFN) α-2b mimetic peptides with antiviral activity.
Methods:
Selecting IFN receptor-binding peptides from a phage-display heptapeptide library using a novel functional biopanning method. This method was developed to identify peptides with activity against vesicular stomatitis virus (VSV) inducing cytopathic effects on WISH cells.
Results:
Sixteen positive clones were obtained after 3 rounds of functional selection. Ten clones were picked from these positive clones according to the results of phage ELISA and were sequenced. The amino acid sequences homologous to IFNα-2b were defined by residues AB loop 31–37, BC loop 68–74, C helix 93–99, CD loop 106–112, D helix 115–121, DE loop 132–138, and E helix 143–161. Two of the peptides, designated clones T3 and T9, aligned with the IFNAR2-binding domains (AB loop and E helix), were synthesized and designated as IR-7 and KP-7, respectively. Both KP-7 and IR-7 were found to compete with GFP/IFNα-2b for receptor binding and mimicked the antiviral activity of IFNα-2b cooperatively.
Conclusion:
Two IFNα-2b mimetic peptides with antiviral activity were derived from a phage-display heptapeptide library using a novel functional selection method.
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This work was supported in part by the National Key Basic Research Development Program of China (973 Program, No 2007CB914803).
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Zhang, Q., Bai, G., Chen, Jq. et al. Identification of antiviral mimetic peptides with interferon α-2b-like activity from a random peptide library using a novel functional biopanning method. Acta Pharmacol Sin 29, 634–640 (2008). https://doi.org/10.1111/j.1745-7254.2008.00755.x
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DOI: https://doi.org/10.1111/j.1745-7254.2008.00755.x
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